AB272556

Anti-ACSL5抗体

Name: ACSL5抗体 (ab272556)| Abcam中文官网
Brand: Abcam Limited
SKU: AB272556
Price: 5791 CNY
Availability: InStock

Anti-ACSL5 antibody

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(2 Publications)

Rabbit Polyclonal ACSL5 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ACSL5 aa 150-300.

查看别名

ACS5, FACL5, UNQ633/PRO1250, ACSL5, Long-chain-fatty-acid--CoA ligase 5, Arachidonate--CoA ligase, Long-chain acyl-CoA synthetase 5, LACS 5

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL5 antibody (AB272556)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL5 antibody (AB272556)

Paraffin-embedded formalin-fixed human pancreas tissue stained for ACSL5 using ab272556 at 1/20 dilution in immunohistochemical analysis. Shows low expression as expected.

Immunocytochemistry/ Immunofluorescence - Anti-ACSL5 antibody (AB272556)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ACSL5 antibody (AB272556)

PFA fixed, Triton X-100 permeablized U-251 MG (human brain glioma cell line) whole cell lysate labeling ACSL5 (green) using ab272556 at 2 ug/ml in ICC/IF analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL5 antibody (AB272556)

Paraffin-embedded formalin-fixed human small intestine tissue stained for ACSL5 using ab272556 at 1/20 dilution in immunohistochemical analysis.

Unknown

Western blot - Anti-ACSL5 antibody (AB272556)

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Western blot - Anti-ACSL5 antibody (ab272556) at 0.4 µg/mL

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CAPAN-2 (human pancreatic ductal adenocarcinoma cell line) whole cell lysate

Predicted band size: 76 kDa

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Unknown

Western blot - Anti-ACSL5 antibody (AB272556)

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Western blot - Anti-ACSL5 antibody (ab272556) at 0.4 µg/mL

Lane 1:

Vector only transfected HEK293T (human epithelial cell line from embryonic kidney transformed with large T antigen) lysate

Lane 2:

ACSL5 over-expression HEK293T lysate

Predicted band size: 76 kDa

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关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

ICC/IF, WB, IHC-P

applications

免疫原

Recombinant Fragment Protein within Human ACSL5 aa 150-300. The exact immunogen used to generate this antibody is proprietary information.

Q9ULC5

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

ACSL5 also known as acyl-CoA synthetase long-chain family member 5 is an enzyme with a mass of approximately 78 kDa. This enzyme plays a critical role in lipid metabolism converting free long-chain fatty acids into fatty acyl-CoA esters ready for incorporation into complex lipids or for energy production. ACSL5 is expressed in various tissues with significant levels detected in liver adipose tissue and skeletal muscle highlighting its importance in metabolic functions.

Biological function summary

This enzyme participates in the activation of fatty acids an important step in lipid biosynthesis and degradation. ACSL5 is part of the larger acyl-CoA synthetase family known for its involvement in lipid metabolism. By converting fatty acids into their acyl-CoA derivatives it provides substrates for lipid synthesis and β-oxidation enabling efficient energy utilization and storage in cells.

Pathways

ACSL5 integrates into both the lipogenesis and β-oxidation pathways. These pathways are essential for maintaining energy balance and lipid homeostasis in cells. In lipogenesis ACSL5 assists in the synthesis of complex lipids by providing activated fatty acids. During β-oxidation it collaborates with enzymes like CPT1 which transports fatty acids into mitochondria for degradation illustrating ACSL5’s integral role in these metabolic processes.

Disruptions in ACSL5 function have associations with metabolic disorders like obesity and non-alcoholic fatty liver disease. These conditions often involve imbalances in lipid processing and storage. ACSL5's interactions with proteins like PPARα a transcription factor involved in lipid metabolism regulation further highlight its relevance in these disorders as alterations in ACSL5 expression can influence PPARα-related lipid metabolic pathways potentially leading to or exacerbating these metabolic diseases.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoAs for both synthesis of cellular lipids, and degradation via beta-oxidation (PubMed : 17681178, PubMed : 22633490, PubMed : 24269233, PubMed : 33191500). ACSL5 may activate fatty acids from exogenous sources for the synthesis of triacylglycerol destined for intracellular storage (By similarity). Utilizes a wide range of saturated fatty acids with a preference for C16-C18 unsaturated fatty acids (By similarity). It was suggested that it may also stimulate fatty acid oxidation (By similarity). At the villus tip of the crypt-villus axis of the small intestine may sensitize epithelial cells to apoptosis specifically triggered by the death ligand TRAIL. May have a role in the survival of glioma cells.

See full target information ACSL5

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:112742 PubMed40546938

2025

Interferon gamma induced-ACSL5 shapes the lipidome of kidney tubular cells.

Applications

Unspecified application

Species

Unspecified reactive species

Virginie Poindessous,Julio L Sampaio,Lydia Bouidghaghen,Ivan Nemazanyy,Alexandre Pallet,Maarten Naesens,Thibaut Vaulet,Dany Anglicheau,Nicolas Pallet

European journal of histochemistry : EJH 67: PubMed37946526

2023

Regulatory roles of ACSL5 in the anti-tumor function of palmitic acid (C16:0) via the ERK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jiapei Lv,Wang Yanting,Shan Wei

View all publications

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Anti-ACSL5抗体

Anti-ACSL5 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL5 antibody (AB272556)

Immunocytochemistry/ Immunofluorescence - Anti-ACSL5 antibody (AB272556)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL5 antibody (AB272556)

Western blot - Anti-ACSL5 antibody (AB272556)

Western blot - Anti-ACSL5 antibody (AB272556)