AB249270

重组Anti-ACOT7抗体[EPR11317(B)] - BSA and Azide free

Anti-ACOT7 antibody [EPR11317(B)] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal ACOT7 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.

查看别名

BACH, ACOT7, Cytosolic acyl coenzyme A thioester hydrolase, Acyl-CoA thioesterase 7, Brain acyl-CoA hydrolase, CTE-IIa, Long chain acyl-CoA thioester hydrolase, hBACH, CTE-II

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Immunoprecipitation - Anti-ACOT7 antibody [EPR11317(B)] - BSA and Azide free (AB249270)

This data was developed using ab156576, the same antibody clone in a different buffer formulation.WB analysis of immunoprecipites
ab156576, at a 1/1000 dilution, staining ACOT7 in Jurkat cell lysate immunoprecipitated using ab156576 at 1/100 dilution.

All lanes:

Immunoprecipitation - Anti-ACOT7 antibody [EPR11317(B)] (<a href='/products/primary-antibodies/acot7-antibody-epr11317b-ab156576'>ab156576</a>)

Predicted band size: 42 kDa

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Unknown

Western blot - Anti-ACOT7 antibody [EPR11317(B)] - BSA and Azide free (AB249270)

This data was developed using ab156576, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ACOT7 antibody [EPR11317(B)] (<a href='/products/primary-antibodies/acot7-antibody-epr11317b-ab156576'>ab156576</a>) at 1/1000 dilution

Lane 1:

fetal brain cell lysate at 10 µg/mL

Lane 2:

A375 cell lysate at 10 µg/mL

Lane 3:

293T cell lysate at 10 µg/mL

Lane 4:

Jurkat cell lysate at 10 µg/mL

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 42 kDa

false

不同偶联物与剂型 (1)

Unconjugated

Anti-ACOT7 antibody [EPR11317(B)]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR11317(B)

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

WB, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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产品详情

ab249270 is the carrier-free version of ab156576.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

ACOT7 also known as cytosolic acyl-CoA thioesterase 7 plays an important role in lipid metabolism by hydrolyzing acyl-CoA thioesters into free fatty acids and CoA. This enzyme possesses a molecular mass of approximately 44 kilodaltons. ACOT7 primarily expresses in the brain with significant levels in neurons. This distribution suggests involvement in processes related to brain lipid metabolism and energy homeostasis.

Biological function summary

ACOT7 influences the regulation of lipid pools and fatty acid signaling in neural cells. It participates in the modulation of free fatty acid concentrations affecting energy metabolism and neuronal function. While ACOT7 does not typically associate with larger protein complexes its enzymatic action impacts the availability and signaling of lipid molecules within the cell potentially influencing cellular responses and energy dynamics.

Pathways

ACOT7 plays a role in fatty acid metabolism and energy homeostasis pathways. It connects with lipid metabolism processes involving enzymes like fatty acid synthase and acyl-CoA synthetase enzymes. By modulating the levels of acyl-CoA and free fatty acids ACOT7 affects broader metabolic pathways that control cellular energy balance and lipid synthesis in brain tissues.

ACOT7 links to neurological conditions and energy metabolism disorders. Its alteration associates with neurodegenerative diseases such as Alzheimer's disease due to its role in maintaining neuronal lipid balance. Furthermore conditions like metabolic syndrome may relate to changes in ACOT7 function given its impact on lipid metabolism. Proteins like amyloid-beta precursor protein in Alzheimer's disease have connections to ACOT7 through these metabolic processes.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Catalyzes the hydrolysis of acyl-CoAs into free fatty acids and coenzyme A (CoASH), regulating their respective intracellular levels (PubMed : 10578051). Preferentially hydrolyzes palmitoyl-CoA, but has a broad specificity acting on other fatty acyl-CoAs with chain-lengths of C8-C18 (PubMed : 10578051). May play an important physiological function in brain (PubMed : 10578051).

See full target information ACOT7

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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重组Anti-ACOT7抗体[EPR11317(B)] - BSA and Azide free