Acid sphingomyelinase抗体[OTI3H7] - BSA and Azide free| Abcam中文官网

产品名称

Anti-Acid sphingomyelinase抗体[OTI3H7] - BSA and Azide free
参阅全部 Acid sphingomyelinase 一抗
描述

小鼠单克隆抗体[OTI3H7] to Acid sphingomyelinase - BSA and Azide free
宿主

Mouse
经测试应用

适用于: WB, IHC-Pmore details
种属反应性

与反应: Human
免疫原

Recombinant fragment corresponding to Human Acid sphingomyelinase aa 50-350. NP_000534 Produced in E.coli.
Database link: P17405

Run BLAST with Run BLAST with
阳性对照
- IHC-P: Human breast adenocarcinoma, lymphoma, colon adenocarcinoma, lung carcinoma, endometrium adenocarcinoma, liver carcinoma, thyroid carcinoma, prostate carcinoma, ovary adenocarcinoma, kidney carcinoma, pancreas, ovary, lymph node, lung, colon, endometrium, liver, prostate, bladder, kidney and tonsil tissue. WB: pCMV6-ENTRY Acid sphingomyelinase cDNA transfected HEK-293T cell lysate.
常规说明

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液

pH: 7.3
Constituent: PBS
无载体

是
Concentration information loading...
纯度

Protein A/G purified
纯化说明

Purified from TCS.
克隆

单克隆
克隆编号

OTI3H7
同种型

IgG1
研究领域
- Neuroscience
- Processes

Compatible Secondaries
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
- Goat Anti-Mouse IgG H&L (HRP) (ab205719)

The Abpromise guarantee

Abpromise™承诺保证使用ab273656于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
WB		1/2000. Predicted molecular weight: 70 kDa.
IHC-P		1/150. Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3 minutes.

说明
WB 1/2000. Predicted molecular weight: 70 kDa.
IHC-P 1/150. Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3 minutes.

功能

Converts sphingomyelin to ceramide. Also has phospholipase C activities toward 1,2-diacylglycerolphosphocholine and 1,2-diacylglycerolphosphoglycerol. Isoform 2 and isoform 3 have lost catalytic activity.
疾病相关

Defects in SMPD1 are the cause of Niemann-Pick disease type A (NPDA) [MIM:257200]; also known as Niemann-Pick disease classical infantile form. It is an early-onset lysosomal storage disorder caused by failure to hydrolyze sphingomyelin to ceramide. It results in the accumulation of sphingomyelin and other metabolically related lipids in reticuloendothelial and other cell types throughout the body, leading to cell death. Niemann-Pick disease type A is a primarily neurodegenerative disorder characterized by onset within the first year of life, mental retardation, digestive disorders, failure to thrive, major hepatosplenomegaly, and severe neurologic symptoms. The severe neurological disorders and pulmonary infections lead to an early death, often around the age of four. Clinical features are variable. A phenotypic continuum exists between type A (basic neurovisceral) and type B (purely visceral) forms of Niemann-Pick disease, and the intermediate types encompass a cluster of variants combining clinical features of both types A and B.
Defects in SMPD1 are the cause of Niemann-Pick disease type B (NPDB) [MIM:607616]; also known as Niemann-Pick disease visceral form. It is a late-onset lysosomal storage disorder caused by failure to hydrolyze sphingomyelin to ceramide. It results in the accumulation of sphingomyelin and other metabolically related lipids in reticuloendothelial and other cell types throughout the body, leading to cell death. Clinical signs involve only visceral organs. The most constant sign is hepatosplenomegaly which can be associated with pulmonary symptoms. Patients remain free of neurologic manifestations. However, a phenotypic continuum exists between type A (basic neurovisceral) and type B (purely visceral) forms of Niemann-Pick disease, and the intermediate types encompass a cluster of variants combining clinical features of both types A and B. In Niemann-Pick disease type B, onset of the first symptoms occurs in early childhood and patients can survive into adulthood.
序列相似性

Belongs to the acid sphingomyelinase family.
Contains 1 saposin B-type domain.
细胞定位

Lysosome.
Target information above from: UniProt accession P17405 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 6609 Human
- Omim: 607608 Human
- SwissProt: P17405 Human
- Unigene: 498173 Human
别名
- Acid sphingomyelinase antibody
- ASM antibody
- ASM_HUMAN antibody
- aSMase antibody
- NPD antibody
- Smpd1 antibody
- Sphingomyelin phosphodiesterase 1 acid lysosomal antibody
- Sphingomyelin phosphodiesterase antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (ab273656)

Paraffin-embedded human tonsil tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.
Western blot - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (ab273656)

All lanes : Same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide at 1/2000 dilution

Lane 1 : pCMV6-ENTRY control cDNA transfected HEK-293T cell lysate
Lane 2 : pCMV6-ENTRY Acid sphingomyelinase cDNA transfected HEK-293T cell lysate

Lysates/proteins at 5 µg per lane.

Predicted band size: 70 kDa

Cells were transfected with cDNA for 48 hrs and lysed.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (ab273656)

Paraffin-embedded human thyroid carcinoma tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (ab273656)

Paraffin-embedded human prostate tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (ab273656)

Paraffin-embedded human prostate carcinoma tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (ab273656)

Paraffin-embedded human pancreas tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (ab273656)

Paraffin-embedded human ovary tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (ab273656)

Paraffin-embedded human ovary adenocarcinoma tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (ab273656)

Paraffin-embedded human lymphoma tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (ab273656)

Paraffin-embedded human lymph node tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (ab273656)

Paraffin-embedded human lung tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (ab273656)

Paraffin-embedded human lung carcinoma tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (ab273656)

Paraffin-embedded human liver tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (ab273656)

Paraffin-embedded human liver carcinoma tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (ab273656)

Paraffin-embedded human kidney tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (ab273656)

Paraffin-embedded human kidney carcinoma tissue stained for Acid sphingomyelinase aab273656 at 1/150 diution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (ab273656)

Paraffin-embedded human endometrium tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (ab273656)

Paraffin-embedded human endometrium adenocarcinoma tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (ab273656)

Paraffin-embedded human colon tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (ab273656)

Paraffin-embedded human colon adenocarcinoma tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (ab273656)

Paraffin-embedded human breast adenocarcinoma tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (ab273656)

Paraffin-embedded human bladder tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheet download

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Key features and details

概述

产品名称

描述

宿主

经测试应用

种属反应性

免疫原

阳性对照

常规说明

性能

形式

存放说明

存储溶液

无载体

纯度

纯化说明

克隆

克隆编号

同种型

研究领域

相关产品

Compatible Secondaries

应用

The Abpromise guarantee

靶标

功能

疾病相关

序列相似性

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

Datasheet download

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