Anti-acetyl Lysine抗体- ChIP Grade (ab21623)
Key features and details
- Rabbit polyclonal to acetyl Lysine - ChIP Grade
- Suitable for: IP, IHC-P, ChIP, ICC/IF, WB, ELISA
- Reacts with: Species independent
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-acetyl Lysine抗体- ChIP Grade
参阅全部 acetyl Lysine 一抗 -
描述
兔多克隆抗体to acetyl Lysine - ChIP Grade -
宿主
Rabbit -
特异性
Recognises proteins acetylated on lysine residues. Tested: acetylated histone, acetylated BSA, and acetylated MBP, no reaction to the non acetylated proteins. -
经测试应用
适用于: IP, IHC-P, ChIP, ICC/IF, WB, ELISAmore details -
种属反应性
与反应: Species independent -
免疫原
Chemical/ Small Molecule corresponding to acetyl Lysine conjugated to keyhole limpet haemocyanin. Acetylated KLH conjugates.
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常规说明
Use to detect acetylation of lysine.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 6.00
Preservative: 0.1% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
The antibody was specifically purified with immobilised acetylated lysine on agarose -
Primary antibody说明
Use to detect acetylation of lysine. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab21623于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP | (1) |
Use at an assay dependent concentration.
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| IHC-P |
Use at an assay dependent concentration. PubMed: 24160175
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| ChIP | (1) |
Use 2-4 µg for 25 µg of chromatin.
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| ICC/IF | (1) |
1/100 - 1/250. Subconfluent MMRU cells seeded in a 6-well plate with or without HDAC inhibitor treatment were fixed and permeabilized with 1% formaldehyde and 0.5% Triton X-100 for 10 minutes and blocked with 10 mg mL-1 BSA for 1 hour at room temperature. Cells were stained with FITC-conjugated anti-acetylated lysine antibody at 1:100 dilution with PBSt 1 % BSA for 1 hour at room temperature.
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| WB | (7) |
1/1000 - 1/2500.
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| ELISA |
1/5000.
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| 说明 |
|---|
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IP
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. PubMed: 24160175 |
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ChIP
Use 2-4 µg for 25 µg of chromatin. |
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ICC/IF
1/100 - 1/250. Subconfluent MMRU cells seeded in a 6-well plate with or without HDAC inhibitor treatment were fixed and permeabilized with 1% formaldehyde and 0.5% Triton X-100 for 10 minutes and blocked with 10 mg mL-1 BSA for 1 hour at room temperature. Cells were stained with FITC-conjugated anti-acetylated lysine antibody at 1:100 dilution with PBSt 1 % BSA for 1 hour at room temperature. |
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WB
1/1000 - 1/2500. |
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ELISA
1/5000. |
靶标
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相关性
In the nucleus, DNA is tightly packed into nucleosomes generating an environment which is highly repressive towards DNA processes such as transcription. Acetylation of lysine residues within proteins has emerged as an important mechanism used by cells to overcome this repression. The acetylation of non-histone proteins such as transcription factors, as well as histones appears to be involved in this process. Acetylation may result in structural transitions as well as specific signaling within discrete chromatin domains. The role of acetylation in intracellular signaling has been inferred from the binding of acetylated peptides by the conserved bromodomain. Furthermore, recent findings suggest that bromodomain/acetylated-lysine recognition can serve as a regulatory mechanism in protein-protein interactions in numerous cellular processes such as chromatin remodeling and transcriptional activation. The reversible lysine acetylation of histones and non-histone proteins plays a vital role in the regulation of many cellular processes including chromatin dynamics and transcription, gene silencing, cell cycle progression, apoptosis, differentiation, DNA replication, DNA repair, nuclear import, and neuronal repression. More than 20 acetyltransferases and 18 deacetylases have been identified so far, but the mechanistic details of substrate selection and site specificity of these enzymes remain unclear. Over 40 transcription factors and 30 other nuclear, cytoplasmic, bacterial, and viral proteins have been shown to be acetylated in vivo. -
别名
- pan acetyl Lysine antibody
图片
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ChIP - Anti-acetyl Lysine antibody - ChIP Grade (ab21623)
Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25µg of chromatin, 2µg of ab21623 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.
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Western blot - Anti-acetyl Lysine antibody - ChIP Grade (ab21623)Primary: All Lanes: Anti acetyl Lysine antibody (ab21623) at 1:1000. Lane 1: Marker. Lane 2: HeLa cells vehicle-treated (ab139414). Lane 3: HeLa cells, trichostatin A-treated (ab139414). Lysates at 20 ug/lane . Secondary: All Lanes: Goat anti-Rabbit IgG 1:10000. Performed under reducing conditions. Blocking buffer: 5% milk in PBS. Observed band sizes: 11 kDa 15kDa 45kDa 50 kDa. -
Western blot - Anti-acetyl Lysine antibody - ChIP Grade (ab21623)Lane 1 = Extract of Mcf7 cells incubated with vehicle 20 ug. Lane 2 = Extract of Mcf7 cells incubated with trichostatin A 20 ug. Lane 3 = Extract of Mcf7 cells incubated with EX527 20 ug. Lane 4 = Extract of Mcf7 cells incubated with nicotinamide 20 ug. Lane 5 = Extract of Mcf7 cells incubated with camptothecin 20 ug. Lane 6 = Extract of Mcf7 cells incubated with camptothecin and trichostatin A 20 ug. Lane 7 = Extract of Mcf7 cells incubated with camptothecin and EX527 20 ug. Lane 8 = Extract of Mcf7 cells incubated with camptothecin and nicotinamide 20 ug. Lane 9 = Extract of 293T cells incubated with vehicle 20 ug. Lane 10 = Extract of 293T cells incubated with camptothecin 20 ug. Lane 11 = Extract of 293T cells incubated with camptothecin and trichostatin A 20 ug. Lane 12 = Extract of 293T cells incubated with camptothecin and EX527 20 ug. Lane 13 = Extract of 293T cells incubated with camptothecin and nicotinamide 20 ug
SDS PAGE performed under reducing conditions (100mM DTT Sample heated at 50°C). Primary : Lanes 1-13: Rabbit anti acetyl Lysine antibody (ab21623) at 1/500 dilution. Secondary : Lanes 1-13: Goat anti rabbit IgG(H&L)-IR680 at 1:10,000 (in green). Developed: Oddysey. Blocking: in 5% Milk + PBS for 3 hours at RT. Primary antibody: in 5% BSA + 50mM Tris pH 7.5 + 150 mM NaCl + 0.05% Tween-20. Secondary antibody: in 5% Milk + PBS + 0.1% Tween-20 + 0.01%SDS for 2 hour at RT. Predicted band size : multiple. Observed band size : multiple -
Western blot - Anti-acetyl Lysine antibody - ChIP Grade (ab21623)All lanes : Anti-acetyl Lysine antibody - ChIP Grade (ab21623) at 0.5 µg/ml
Lane 1 : Untreated Human melanoma cell lysate
Lane 2 : TSA-treated Human melanoma cell lysate
Lysates/proteins at 75 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP at 0.25 µg/ml
Developed using the ECL technique.
Observed band size: 16-18 kDa why is the actual band size different from the predicted?
Additional bands at: 12-14 kDa. We are unsure as to the identity of these extra bands. -
Immunocytochemistry/ Immunofluorescence - Anti-acetyl Lysine antibody - ChIP Grade (ab21623)Immunofluorescent staining of Human melanoma cells, using Rabbit polyclonal to acetyl Lysine (ab21623) at 1:100 dilution.
1: Untreated cells
2: TSA treated cells
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Immunoprecipitation - Anti-acetyl Lysine antibody - ChIP Grade (ab21623)p53 acetylation upon Doxorubicin treatment in human melanoma cells (MMRU cells). MMRU cells were treated with 0.5 ug/ml Dox for various times and lyzed for whole protein. Immunoprecipitation was performed with ab21623. Western blot was performed to detect the immunoprecipitated p53 with anti-p53 antibody.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (67)
ab21623 被引用在 67 文献中.
- Liu D et al. Anemonin inhibits sepsis-induced acute kidney injury via mitigating inflammation and oxidative stress. Biotechnol Appl Biochem 70:1983-2001 (2023). PubMed: 37592376
- Yu A et al. Klf4-Sirt3/Pparα-Lcad pathway contributes to high phosphate-induced lipid degradation. Cell Commun Signal 21:5 (2023). PubMed: 36624473
- Liu LW et al. Gut microbiota-derived nicotinamide mononucleotide alleviates acute pancreatitis by activating pancreatic SIRT3 signalling. Br J Pharmacol 180:647-666 (2023). PubMed: 36321732
- Zhao J et al. Mitochondrial PKM2 deacetylation by procyanidin B2-induced SIRT3 upregulation alleviates lung ischemia/reperfusion injury. Cell Death Dis 13:594 (2022). PubMed: 35821123
- Zhang J et al. Adiponectin ameliorates hypertrophic scar by inhibiting Yes-associated protein transcription through SIRT1-mediated deacetylation of C/EBPβ and histone H3. iScience 25:105236 (2022). PubMed: 36274941