Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
使用敲除细胞株进行验证重组RabMAb

重组Anti-Acetyl Coenzyme A Carboxylase (phospho S79)抗体[EP1885Y] - BSA and Azide free (ab173583)

Submit a review Submit a question References (1)
Western blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] - BSA and Azide free (ab173583)
  • Dot Blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] - BSA and Azide free (ab173583)
  • Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] - BSA and Azide free (ab173583)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1885Y] to Acetyl Coenzyme A Carboxylase (phospho S79) - BSA and Azide free
  • Suitable for: Dot blot, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-Acetyl Coenzyme A Carboxylase (phospho S79)抗体[EP1885Y] - BSA and Azide free
    参阅全部 Acetyl Coenzyme A Carboxylase 一抗

  • 描述

    兔单克隆抗体[EP1885Y] to Acetyl Coenzyme A Carboxylase (phospho S79) - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Dot blot, WBmore details
    不适用于: Flow Cyt,ICC/IF,IHC-P or IP

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: HEK293 and HAP1 cell lysates.

  • 常规说明

    ab173583 is the carrier-free version of ab68191.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab173583于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Dot blot
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 265 kDa (predicted molecular weight: 277 kDa).
说明
Dot blot
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 265 kDa (predicted molecular weight: 277 kDa).
应用说明
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

靶标

图片

  • Western blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] - BSA and Azide free (ab173583)
    Western blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] - BSA and Azide free (ab173583)
    All lanes : Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191) at 1/5000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : ACACA knockout HAP1 whole cell lysate
    Lane 3 : HEK293 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 277 kDa
    Observed band size: 266 kDa why is the actual band size different from the predicted?



    Lanes 1 - 3: Merged signal (red and green). Green - ab68191 observed at 266 kDa. Red - loading control, ab130007, observed at 130 kDa.

    ab68191 was shown to specifically react with in wild-type HAP1 cells as signal was lost in ACACA knockout cells. Wild-type and ACACA knockout samples were subjected to SDS-PAGE. Ab68191 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab68191).

  • Dot Blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] - BSA and Azide free (ab173583)
    Dot Blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] - BSA and Azide free (ab173583)

    Dot blot analysis of Acetyl Coenzyme A Carboxylase (pS79) phospho peptide (lane 1) and Acetyl Coenzyme A Carboxylase non-phospho peptide (lane 2) labelling Acetyl Coenzyme A Carboxylase (phospho S79) with ab68191 at a dilution of 1/1000. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/2500).

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab68191).

  • Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] - BSA and Azide free (ab173583)
    Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] - BSA and Azide free (ab173583)

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (1)

发表研究结果有使用 ab173583？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab173583 被引用在 1 文献中.

  • Zhao T  et al. TFEC contributes to cardiac hypertrophy by inhibiting AMPK/mTOR signaling. Exp Ther Med 22:1271 (2021). PubMed: 34594408

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

There are currently no Customer reviews or Questions for ab173583.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com