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重组RabMAb

重组Anti-Acetyl Coenzyme A carboxylase alpha抗体[EPR23235-47] - BSA and Azide free (ab272703)

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Immunoprecipitation - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)
  • Immunoprecipitation - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)
  • Flow Cytometry (Intracellular) - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)
  • Immunocytochemistry/ Immunofluorescence - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)
  • Immunocytochemistry/ Immunofluorescence - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)
  • Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23235-47] to Acetyl Coenzyme A carboxylase alpha - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra), IP
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-Acetyl Coenzyme A carboxylase alpha抗体[EPR23235-47] - BSA and Azide free
    参阅全部 Acetyl Coenzyme A carboxylase alpha 一抗

  • 描述

    兔单克隆抗体[EPR23235-47] to Acetyl Coenzyme A carboxylase alpha - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IHC-P, ICC/IF, Flow Cyt (Intra), IPmore details
    不适用于: IHC-Fr

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: 293T, HeLa, PC-12 and C2C12 lysates. IHC-P: Human breast carcinoma and endometrial carcinoma tissue. Mouse and rat lung tissue. ICC/IF: C2C12 and 293T cells. Flow Cyt (intra): C2C12 and 293T cells. IP: C2C12 and 293T cells.

  • 常规说明

    ab272703 is the carrier-free version of ab269272.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab272703于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use at an assay dependent concentration. Predicted molecular weight: 265 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
Flow Cyt (Intra)
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
说明
WB
Use at an assay dependent concentration. Predicted molecular weight: 265 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
Flow Cyt (Intra)
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
应用说明
Is unsuitable for IHC-Fr.

靶标

  • 功能

    Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase.

  • 组织特异性

    Expressed in brain, placental, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver.

  • 通路

    Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.

  • 疾病相关

    Defects in ACACA are a cause of acetyl-CoA carboxylase 1 deficiency (ACACAD) [MIM:200350]; also known as ACAC deficiency or ACC deficiency. An inborn error of de novo fatty acid synthesis associated with severe brain damage, persistent myopathy and poor growth.

  • 序列相似性

    Contains 1 ATP-grasp domain.
    Contains 1 biotin carboxylation domain.
    Contains 1 biotinyl-binding domain.
    Contains 1 carboxyltransferase domain.

  • 翻译后修饰

    Phosphorylation on Ser-1263 is required for interaction with BRCA1.

  • 细胞定位

    Cytoplasm.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • ACAC antibody
    • ACACA antibody
    • ACACA_HUMAN antibody
    • ACC alpha antibody
    • ACC-alpha antibody
    • ACC1 antibody
    • ACCA antibody
    • Acetyl Coenzyme A antibody
    • Biotin carboxylase antibody
    see all

图片

  • Immunoprecipitation - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)
    Immunoprecipitation - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)

    Acetyl Coenzyme A carboxylase alpha was immunoprecipitated from 0.35 mg 293T (human embryonic kidney epithelial cell), whole cell lysate 10 µg with ab269272 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab269272 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: 293T whole cell lysate 10 µg.

    Lane 2: ab269272 IP in 293T whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab269272 in 293T whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 8 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269272).

  • Immunoprecipitation - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)
    Immunoprecipitation - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)

    Acetyl Coenzyme A carboxylase alpha was immunoprecipitated from 0.35 mg C2C12 (mouse myoblasts myoblast), whole cell lysate 10 µg with ab269272 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab269272 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: C2C12 whole cell lysate 10 µg.

    Lane 2: ab269272 IP in C2C12 whole cell lysate 10 µg.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab269272 in C2C12 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 8 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269272).

  • Flow Cytometry (Intracellular) - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)
    Flow Cytometry (Intracellular) - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)

    Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized 293T (Human embryonic kidney epithelial cell) cells labelling Acetyl Coenzyme A carboxylase alpha with ab269272 at 1/500 dilution (0.1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269272). 

     

     

  • Immunocytochemistry/ Immunofluorescence - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)
    Immunocytochemistry/ Immunofluorescence - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized 293T cells labelling Acetyl Coenzyme A carboxylase alpha with ab269272 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 µg/ml dilution (Green). Confocal image showing cytoplasmic staining in 293T cell line. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 µg/ml dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 2 µg/ml dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269272).

  • Immunocytochemistry/ Immunofluorescence - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)
    Immunocytochemistry/ Immunofluorescence - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C2C12 cells labelling Acetyl Coenzyme A carboxylase alpha with ab269272 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 µg/ml dilution (Green). Confocal image showing cytoplasmic staining in C2C12 cell line. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 µg/ml dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 2 µg/ml dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269272).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)

    Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling Acetyl Coenzyme A carboxylase alpha with ab269272 at 1/500 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat lung (PMID: 17521700). The section was incubated with ab269272 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269272).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Acetyl Coenzyme A carboxylase alpha with ab269272 at 1/500 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse lung (PMID: 17521700). The section was incubated with ab269272 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269272).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)

    Immunohistochemical analysis of paraffin-embedded human endometrial carcinoma tissue labeling Acetyl Coenzyme A carboxylase alpha with ab269272 at 1/500 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human endometrial carcinoma. The section was incubated with ab269272 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269272).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling Acetyl Coenzyme A carboxylase alpha with ab269272 at 1/500 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human breast carcinoma (PMID: 21415164). The section was incubated with ab269272 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269272).

  • Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)
    Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

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