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使用敲除细胞株进行验证重组RabMAb

重组Anti-ACE2抗体[EPR24705-45] (ab272500)

Reviews (2) Submit a question References (6)
Immunocytochemistry/ Immunofluorescence - Anti-ACE2 antibody [EPR24705-45] (ab272500)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR24705-45] (ab272500)
  • Immunocytochemistry/ Immunofluorescence - Anti-ACE2 antibody [EPR24705-45] (ab272500)
  • Immunocytochemistry/ Immunofluorescence - Anti-ACE2 antibody [EPR24705-45] (ab272500)
  • Flow Cytometry - Anti-ACE2 antibody [EPR24705-45] (ab272500)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR24705-45] (ab272500)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR24705-45] (ab272500)
  • Anti-ACE2 antibody [EPR24705-45] (ab272500)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24705-45] to ACE2
  • Suitable for: Flow Cyt, IP, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 Carrier Free

概述

  • 产品名称

    Anti-ACE2抗体[EPR24705-45]
    参阅全部 ACE2 一抗

  • 描述

    兔单克隆抗体[EPR24705-45] to ACE2

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt, IP, IHC-P, ICC/IFmore details
    不适用于: IHC-Fr or WB

  • 种属反应性

    与反应: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: Human kidney and testis tissue lysates; Calu-3 whole cell lysate. IHC-P: Human kidney and testis tissues. ICC/IF: Calu-3, HEK-293T cells transfected with myc-ACE-2; WT CaCo2. Flow cyt: Calu-3 cells. IP: Human testis tissue lysate.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab272500于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (1)
1/500.
IP
1/30.
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/50.
说明
Flow Cyt
1/500.
IP
1/30.
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/50.
应用说明
Is unsuitable for IHC-Fr or WB.

靶标

  • 功能

    Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses.

  • 组织特异性

    Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in heart, kidney, testis, and gastrointestinal system.

  • 序列相似性

    Belongs to the peptidase M2 family.

  • 翻译后修饰

    N-glycosylation on Asn-90 may limit SARS infectivity.

  • 细胞定位

    Secreted and Cell membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • ACE 2 antibody
      • ACE related carboxypeptidase antibody
      • ACE-related carboxypeptidase antibody
      • ACE2 antibody
      • ACE2_HUMAN antibody
      • ACEH antibody
      • Angiotensin converting enzyme 2 antibody
      • Angiotensin converting enzyme homolog antibody
      • Angiotensin converting enzyme like protein antibody
      • Angiotensin I Converting Enzyme (peptidyl dipeptidase A) 2 antibody
      • Angiotensin I converting enzyme 2 antibody
      • Angiotensin-converting enzyme homolog antibody
      • DKFZP434A014 antibody
      • EC 3.4.17 antibody
      • metalloprotease MPROT 15 antibody
      • Metalloprotease MPROT15 antibody
      • OTTHUMP00000022963 antibody
      • Processed angiotensin-converting enzyme 2 antibody
      see all

    图片

    • Immunocytochemistry/ Immunofluorescence - Anti-ACE2 antibody [EPR24705-45] (ab272500)
      Immunocytochemistry/ Immunofluorescence - Anti-ACE2 antibody [EPR24705-45] (ab272500)

      ab272500 staining ACE2 in wild-type Caco2 cells (top panel) and ACE2 knockout Caco2 cells (ab273731) (bottom panel). The cells were fixed with 4% paraformaldehyde (10 min) then permeabilized with 0.1% Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab272500 at 10μg/ml concentration and ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
      Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR24705-45] (ab272500)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR24705-45] (ab272500)

      Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling ACE2 with ab272500 at 1/100 (5.0 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Membranous and cytoplasmic staining in human testis. The section was incubated with ab272500 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) .

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Immunocytochemistry/ Immunofluorescence - Anti-ACE2 antibody [EPR24705-45] (ab272500)
      Immunocytochemistry/ Immunofluorescence - Anti-ACE2 antibody [EPR24705-45] (ab272500)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T cells labelling ACE2 with ab272500 at 1/250 (2.0 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing positive staining in HEK-293T cells transfected with myc-tagged ACE2 expression vector. Myc-Tag Mouse mAb (Alexa Fluor® 647) was used at 1/100 dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    • Immunocytochemistry/ Immunofluorescence - Anti-ACE2 antibody [EPR24705-45] (ab272500)
      Immunocytochemistry/ Immunofluorescence - Anti-ACE2 antibody [EPR24705-45] (ab272500)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Calu-3 cells labelling ACE2 with ab272500 at 1/50 (10.0 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in subsets of Calu-3 cells. Negative control: HeLa (PMID: 32926098). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) (Red) was used at 1/200 dilution. The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    • Flow Cytometry - Anti-ACE2 antibody [EPR24705-45] (ab272500)
      Flow Cytometry - Anti-ACE2 antibody [EPR24705-45] (ab272500)

      Flow cytometric analysis of HeLa (Human cervix adenocarcinoma epithelial cell, Left)/ Calu-3 (human lung adenocarcinoma epithelial cell, Right) cells labelling ACE2 with ab272500 at 1/500 dilution (0.1ug). Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution was used as the secondary antibody.

      Negative control: HeLa (PMID: 32926098). Gated on viable cells.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR24705-45] (ab272500)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR24705-45] (ab272500)

      Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling ACE2 with ab272500 at 1/100 (5.0 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous and cytoplasmic staining in human kidney. The section was incubated with ab272500 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND™ RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) .

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR24705-45] (ab272500)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR24705-45] (ab272500)

      Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling ACE2 with ab272500 at 1/100 (5.0 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining in human liver. The section was incubated with ab272500 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond®Polymer Refine Detection) .

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Anti-ACE2 antibody [EPR24705-45] (ab272500)
      Anti-ACE2 antibody [EPR24705-45] (ab272500)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (6)

    发表研究结果有使用 ab272500？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab272500 被引用在 6 文献中.

    • Daniels A  et al. One for all-human kidney Caki-1 cells are highly susceptible to infection with corona- and other respiratory viruses. J Virol 97:e0055523 (2023). PubMed: 37668370
    • McColman S  et al. SARS-CoV-2 virus-like-particles via liposomal reconstitution of spike glycoproteins. Nanoscale Adv 5:4167-4181 (2023). PubMed: 37560413
    • Ma X  et al. SARS-CoV-2 RBD and Its Variants Can Induce Platelet Activation and Clearance: Implications for Antibody Therapy and Vaccinations against COVID-19. Research (Wash D C) 6:0124 (2023). PubMed: 37223472
    • Perico L  et al. SARS-CoV-2 Spike Protein 1 Activates Microvascular Endothelial Cells and Complement System Leading to Platelet Aggregation. Front Immunol 13:827146 (2022). PubMed: 35320941
    • Yao Y  et al. Hydroxychloroquine treatment on SARS-CoV-2 receptor ACE2, TMPRSS2 and NRP1 expression in human primary pterygium and conjunctival cells. Exp Eye Res 214:108864 (2022). PubMed: 34826419
    • Guo X  et al. Control of SARS-CoV-2 infection by MT1-MMP-mediated shedding of ACE2. Nat Commun 13:7907 (2022). PubMed: 36564389

    客户评价及客户问答

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    Flow Cytometry abreview for Anti-ACE2 antibody [EPR24705-45]

     Excellent
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (CaLu-3)
    Permeabilization
    No
    Gating Strategy
    Live cells
    Specification
    CaLu-3
    Preparation
    Cell harvesting/tissue preparation method: Cells were trypsinised
    Sample buffer: PBS + 5% FCS
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Thomas Crozier

    Verified customer

    提交于 Jun 16 2021

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