重组Anti-ACAT2/Acetyl-CoA acetyltransferase抗体[EPR8417] (ab131215)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8417] to ACAT2/Acetyl-CoA acetyltransferase
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-ACAT2/Acetyl-CoA acetyltransferase抗体[EPR8417]
参阅全部 ACAT2/Acetyl-CoA acetyltransferase 一抗 -
描述
兔单克隆抗体[EPR8417] to ACAT2/Acetyl-CoA acetyltransferase -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: ICC/IF -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide within Human ACAT2/Acetyl-CoA acetyltransferase aa 200-300. The exact sequence is proprietary.
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阳性对照
- Human liver tissue; HepG2, HeLa, Jurkat and K562 cell lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 1.91 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR8417 -
同种型
IgG -
研究领域
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipoprotein metabolism
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131215于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (2) |
1/1000 - 1/10000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
|
IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Heat up to 98 degrees C, below boiling, and then let cool for 10-20 mins. |
说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 mins. |
靶标
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序列相似性
Belongs to the thiolase family. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 39 Human
- Entrez Gene: 110460 Mouse
- Entrez Gene: 308100 Rat
- Omim: 100678 Human
- SwissProt: Q9BWD1 Human
- SwissProt: Q8CAY6 Mouse
- SwissProt: Q5XI22 Rat
- Unigene: 571037 Human
see all -
别名
- Acat2 antibody
- acetoacetyl CoA thiolase antibody
- acetoacetyl Coenzyme A thiolase antibody
see all
图片
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All lanes : Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] (ab131215) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 41 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] (ab131215)
Immunohistochemical analysis of paraffin embedded Human liver tissue labelling ACAT2/Acetyl-CoA acetyltransferase with ab131215 antibody at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab131215 被引用在 7 文献中.
- Vergani E et al. Targeting of the Lipid Metabolism Impairs Resistance to BRAF Kinase Inhibitor in Melanoma. Front Cell Dev Biol 10:927118 (2022). PubMed: 35912092
- Wang S et al. Role of sortilin 1 (SORT1) on fatty acid-mediated cholesterol metabolism in primary calf hepatocytes. J Dairy Sci 105:7773-7786 (2022). PubMed: 35940922
- Buettner R et al. Targeting the metabolic vulnerability of acute myeloid leukemia blasts with a combination of venetoclax and 8-chloro-adenosine. J Hematol Oncol 14:70 (2021). PubMed: 33902674
- Zhang Z et al. HFD-induced TRAF6 upregulation promotes liver cholesterol accumulation and fatty liver development via EZH2-mediated miR-429/PPARa axis. Mol Ther Nucleic Acids 24:711-727 (2021). PubMed: 33996254
- Cheng L et al. Circ_RPL23A acts as a miR-1233 sponge to suppress the progression of clear cell renal cell carcinoma by promoting ACAT2. J Bioenerg Biomembr 53:415-428 (2021). PubMed: 34036483
- Tian F et al. MiR-542-5p Inhibits Hyperglycemia and Hyperlipoidemia by Targeting FOXO1 in the Liver. Yonsei Med J 61:780-788 (2020). PubMed: 32882762
- Weng M et al. ACAT2 Promotes Cell Proliferation and Associates with Malignant Progression in Colorectal Cancer. Onco Targets Ther 13:3477-3488 (2020). PubMed: 32425549