AB251082

重组Anti-ACAT2/Acetyl-CoA acetyltransferase抗体[EPR8416(2)] - BSA and Azide free

Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8416(2)] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal ACAT2/Acetyl-CoA acetyltransferase antibody. Carrier free. Suitable for IP, WB and reacts with Human, Rat, Mouse samples. Cited in 1 publication.

查看别名

ACTL, ACAT2, Acetyl-CoA transferase-like protein, Cytosolic acetoacetyl-CoA thiolase

3 Images

Supplier Data

Immunoprecipitation - Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8416(2)] - BSA and Azide free (AB251082)

This data was developed using ab191431, the same antibody clone in a different buffer formulation.

Western blot analysis of immunoprecipitation pellet from HepG2 cell lysate immunoprecipitated using ab191431 at 1/20 dilution (lane 1) or PBS control (lane 2).
Secondary : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8416(2)] (<a href='/products/primary-antibodies/acat2-acetyl-coa-acetyltransferase-antibody-epr84162-ab191431'>ab191431</a>)

Predicted band size: 41 kDa

false

Supplier Data

Western blot - Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8416(2)] - BSA and Azide free (AB251082)

This data was developed using ab191431, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8416(2)] (<a href='/products/primary-antibodies/acat2-acetyl-coa-acetyltransferase-antibody-epr84162-ab191431'>ab191431</a>) at 1/10000 dilution

Lane 1:

HepG2 cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

K562 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 41 kDa

false

Supplier Data

Western blot - Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8416(2)] - BSA and Azide free (AB251082)

This data was developed using ab191431, the same antibody clone in a different buffer formulation.

All lanes:

Lane 1:

PC12 cell lysate at 10 µg

Lane 2:

NIH 3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 41 kDa

false

不同偶联物与剂型 (1)

Unconjugated

Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8416(2)]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR8416(2)

亚型

IgG

不含载体蛋白

Yes

反应种属

Mouse, Rat, Human

应用

IP, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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产品详情

ab251082 is the carrier-free version of ab191431.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

ACAT2 also known as Acetyl-CoA acetyltransferase is an enzyme that plays a role in lipid metabolism catalyzing the conversion of two molecules of acetyl-CoA into acetoacetyl-CoA. It shares functionality with other acetyltransferases such as chloramphenicol acetyltransferase. ACAT2 has a molecular mass of approximately 41 kDa. Expression of ACAT2 is mainly in the liver and the cytosol of cells involved in sterol regulatory processes. It functions as a homotetramer and is essential in maintaining cellular levels of acetyl-CoA and related metabolites.

Biological function summary

This enzyme facilitates the synthesis of cholesterol and ketone bodies essential processes for cellular energy balance. ACAT2 works as an independent enzyme rather than as a component of a multi-enzyme complex unlike some other acetyltransferases. By managing acetyl-CoA levels it supports energy homeostasis and lipogenesis providing substrates for downstream processes that are critical for sustaining cellular function and response to energy demands.

Pathways

The acetyl-transfer reactions catalyzed by ACAT2 are integral to the cholesterol biosynthesis and ketogenesis pathways. These pathways are important for energy balance and lipid modification. Within these pathways ACAT2 acts in close relation with HMG-CoA synthase an important player in cholesterol biosynthesis. The enzyme’s actions provide acetoacetyl-CoA which is a substrate for the production of HMG-CoA highlighting its role in maintaining metabolic flux.

ACAT2's role in cholesterol metabolism links it to atherosclerosis and non-alcoholic fatty liver disease (NAFLD). Disruption in its function can lead to elevated levels of cholesterol and triglycerides risk factors for atherosclerosis. In NAFLD ACAT2 expression may influence lipid accumulation in the liver contributing to disease pathogenesis. Its connection with other proteins like LDL receptor and apolipoprotein B through cholesterol metabolism emphasizes its potential as a therapeutic target for managing lipid-related disorders.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Involved in the biosynthetic pathway of cholesterol.

See full target information ACAT2

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 25:8127-8139 PubMed34350711

2021

Overexpression of let-7d explains down-regulated KDM3A and ENO2 in the pathogenesis of preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Xu,Yonghui Song,Lili Lu

View all publications

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Abcam Product Promise

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详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-ACAT2/Acetyl-CoA acetyltransferase抗体[EPR8416(2)] - BSA and Azide free