Anti-ACADS/SCAD抗体[7E1AB5] (ab110318)
![Western blot - Anti-ACADS/SCAD antibody [7E1AB5] (ab110318)](https://www.abcam.cn/ps/products/110/ab110318/Images/ACADS-Primary-antibodies-ab110318-1.gif "Western blot - Anti-ACADS/SCAD antibody [7E1AB5] (ab110318)")
Key features and details
- Mouse monoclonal [7E1AB5] to ACADS/SCAD
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-ACADS/SCAD抗体[7E1AB5]
参阅全部 ACADS/SCAD 一抗 -
描述
小鼠单克隆抗体[7E1AB5] to ACADS/SCAD -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Fibroblast, HeLa, HepG2. Human Heart FFPE tissue.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
纯度
Proprietary Purification -
纯化说明
The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Purity >95% by SDS-PAGE. -
克隆
单克隆 -
克隆编号
7E1AB5 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab110318于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 44 kDa.
|
|
| ICC/IF |
Use a concentration of 1 µg/ml.
|
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| IHC-P |
Use a concentration of 5 µg/ml.
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| 说明 |
|---|
|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 44 kDa. |
|
ICC/IF
Use a concentration of 1 µg/ml. |
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IHC-P
Use a concentration of 5 µg/ml. |
靶标
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通路
Lipid metabolism; mitochondrial fatty acid beta-oxidation. -
疾病相关
Defects in ACADS are the cause of acyl-CoA dehydrogenase short-chain deficiency (ACADSD) [MIM:201470]. It is an autosomal recessive disorder resulting in acute acidosis and muscle weakness in infants, and a form of lipid-storage myopathy in adults. -
序列相似性
Belongs to the acyl-CoA dehydrogenase family. -
细胞定位
Mitochondrion matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 35 Human
- Omim: 606885 Human
- SwissProt: P16219 Human
- Unigene: 507076 Human
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别名
- ACAD3 antibody
- ACADS antibody
- ACADS_HUMAN antibody
see all
图片
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Western blot - Anti-ACADS/SCAD antibody [7E1AB5] (ab110318)All lanes : Anti-ACADS/SCAD antibody [7E1AB5] (ab110318) at 1 µg/ml
Lane 1 : HepG2 whole cell cells at 10 µg
Lane 2 : ACADS at 0.01 µg
Predicted band size: 44 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-ACADS/SCAD antibody [7E1AB5] (ab110318)](https://www.abcam.cn/ps/products/110/ab110318/Images/ACADS-Primary-antibodies-ab110318-3.gif)
Immunocytochemistry/ Immunofluorescence - Anti-ACADS/SCAD antibody [7E1AB5] (ab110318)Immunocytochemistry image of stained human fibroblasts. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with the ab110318 (1 µg/ml) for 2 hours at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. The target protein locates to the mitochondrial matrix. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADS/SCAD antibody [7E1AB5] (ab110318)](https://www.abcam.cn/ps/products/110/ab110318/Images/ACADS-Primary-antibodies-ab110318-4.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADS/SCAD antibody [7E1AB5] (ab110318)IHC image of ACADS/SCAD staining in Human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab110318, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab110318 被引用在 1 文献中.
- Zhao XH et al. Integrative analysis reveals marker genes for intestinal mucosa barrier repairing in clinical patients. iScience 26:106831 (2023). PubMed: 37250791