重组Anti-ACADM/MCAD抗体[EPR3707] (ab108192)
![Western blot - Anti-ACADM/MCAD antibody [EPR3707] (ab108192)](https://www.abcam.cn/ps/products/108/ab108192/Images/ab108192-120685-anti-acadm-mcad-antibody-epr3707-western-blot.jpg "Western blot - Anti-ACADM/MCAD antibody [EPR3707] (ab108192)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3707] to ACADM/MCAD
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-ACADM/MCAD抗体[EPR3707]
参阅全部 ACADM/MCAD 一抗 -
描述
兔单克隆抗体[EPR3707] to ACADM/MCAD -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human fetal heart, fetal liver, HeLa and HepG2 cell lysates IHC-P: Human kidney and liver tissue
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR3707 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108192于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 47 kDa.
|
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| IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes. |
| 说明 |
|---|
|
WB
1/1000 - 1/5000. Predicted molecular weight: 47 kDa. |
|
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes. |
靶标
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功能
This enzyme is specific for acyl chain lengths of 4 to 16. -
通路
Lipid metabolism; mitochondrial fatty acid beta-oxidation. -
疾病相关
Defects in ACADM are the cause of acyl-CoA dehydrogenase medium-chain deficiency (ACADMD) [MIM:201450]. It is an autosomal recessive disease which causes fasting hypoglycemia, hepatic dysfunction, and encephalopathy, often resulting in death in infancy. -
序列相似性
Belongs to the acyl-CoA dehydrogenase family. -
细胞定位
Mitochondrion matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 34 Human
- Entrez Gene: 11364 Mouse
- Entrez Gene: 24158 Rat
- Omim: 607008 Human
- SwissProt: P11310 Human
- SwissProt: P45952 Mouse
- SwissProt: P08503 Rat
- Unigene: 445040 Human
see all -
别名
- ACAD 1 antibody
- ACAD1 antibody
- Acadm antibody
see all
图片
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Western blot - Anti-ACADM/MCAD antibody [EPR3707] (ab108192)All lanes : Anti-ACADM/MCAD antibody [EPR3707] (ab108192) at 1/1000 dilution
Lane 1 : Human fetal heart tissue lysate
Lane 2 : Human fetal liver tissue lysate
Lane 3 : HeLa cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 47 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADM/MCAD antibody [EPR3707] (ab108192)](https://www.abcam.cn/ps/products/108/ab108192/Images/ab108192-120684-anti-acadm-mcad-antibody-epr3707-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADM/MCAD antibody [EPR3707] (ab108192)Immunohistochemical analysis of ACADM/MCAD in paraffin embedded Human kidney tissue, using ab108192 at a 1/250 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADM/MCAD antibody [EPR3707] (ab108192)](https://www.abcam.cn/ps/products/108/ab108192/Images/ab108192-120683-anti-acadm-mcad-antibody-epr3707-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADM/MCAD antibody [EPR3707] (ab108192)Immunohistochemical analysis of ACADM/MCAD in paraffin embedded Human liver tissue, using ab108192 at a 1/250 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-ACADM/MCAD antibody [EPR3707] (ab108192)](https://www.abcam.cn/ps/products/108/ab108192/Images/ab108192-4-benefits-of-recombinant-antibodies.png)
Anti-ACADM/MCAD antibody [EPR3707] (ab108192)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab108192 尚未被引用在任何文献中。