Anti-ACADL/LCAD抗体[7F5DD6] (ab128566)
Key features and details
- Mouse monoclonal [7F5DD6] to ACADL/LCAD
- Suitable for: WB, IP
- Reacts with: Mouse, Rat
- Isotype: IgG1
概述
-
产品名称
Anti-ACADL/LCAD抗体[7F5DD6]
参阅全部 ACADL/LCAD 一抗 -
描述
小鼠单克隆抗体[7F5DD6] to ACADL/LCAD -
宿主
Mouse -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Mouse, Rat
不与反应: Human -
免疫原
Tissue, cells or virus. This information is considered to be commercially sensitive.
-
阳性对照
- Rat Liver Homogenate, Rat Liver Mitochondria, Mouse Liver Homogenate and Mouse Liver Mitochondria
-
常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: 99% HEPES buffered saline -
Concentration information loading...
-
纯度
Ammonium Sulphate Precipitation -
纯化说明
Purity is near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then concentrated by ammonium sulfate precipitation. -
克隆
单克隆 -
克隆编号
7F5DD6 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab128566于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 47 kDa.
|
|
IP |
Use at an assay dependent concentration.
|
说明 |
---|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 47 kDa. |
IP
Use at an assay dependent concentration. |
靶标
-
通路
Lipid metabolism; mitochondrial fatty acid beta-oxidation. -
疾病相关
Defects in ACADL are a cause of acyl-CoA dehydrogenase very long-chain deficiency (ACADVLD) [MIM:201475]. An inborn error of mitochondrial fatty acid beta-oxidation which leads to impaired long-chain fatty acid beta-oxidation. It is clinically heterogeneous, with three major phenotypes: a severe childhood form characterized by early onset, high mortality and high incidence of cardiomyopathy; a milder childhood form with later onset, characterized by hypoketotic hypoglycemia, low mortality and rare cardiomyopathy; an adult form, with isolated skeletal muscle involvement, rhabdomyolysis and myoglobinuria, usually triggered by exercise or fasting. -
序列相似性
Belongs to the acyl-CoA dehydrogenase family. -
细胞定位
Mitochondrion matrix. - Information by UniProt
-
数据库链接
- Entrez Gene: 11363 Mouse
- Entrez Gene: 25287 Rat
- SwissProt: P51174 Mouse
- SwissProt: P15650 Rat
- Unigene: 2445 Mouse
- Unigene: 174 Rat
-
别名
- ACAD4 antibody
- ACADL antibody
- ACADL_HUMAN antibody
see all
图片
-
All lanes : Anti-ACADL/LCAD antibody [7F5DD6] (ab128566) at 1 µg/ml
Lane 1 : Ladder
Lane 2 : Rat Liver Homogenate at 15 µg
Lane 3 : Rat Liver Mitochondria at 15 µg
Lane 4 : Rat H4IIE cell lysate at 15 µg
Lane 5 : Mouse Liver Homogenate at 15 µg
Lane 6 : Mouse Liver Mitochondria at 15 µg
Lane 7 : Mouse MEF cell lysate at 15 µg
Predicted band size: 47 kDa -
Immunoprecipitation using ab128566
Lane 1: Ladder Lane 2: Rat Liver Homogenate.
数据表及文件
-
Datasheet download
文献 (4)
ab128566 被引用在 4 文献中.
- Zhang Y et al. Combining albumin deficiency and acute exercise reduces hepatic lipid droplet size in mice. Lipids Health Dis 22:78 (2023). PubMed: 37344835
- Laudette M et al. Cyclic AMP-binding protein Epac1 acts as a metabolic sensor to promote cardiomyocyte lipotoxicity. Cell Death Dis 12:824 (2021). PubMed: 34471096
- Maurya SK et al. Sarcolipin Signaling Promotes Mitochondrial Biogenesis and Oxidative Metabolism in Skeletal Muscle. Cell Rep 24:2919-2931 (2018). PubMed: 30208317
- Zhang R et al. Short-term administration of Nicotinamide Mononucleotide preserves cardiac mitochondrial homeostasis and prevents heart failure. J Mol Cell Cardiol 112:64-73 (2017). PubMed: 28882480