重组Anti-ACAA2抗体[EPR6733] (ab128911)
![Western blot - Anti-ACAA2 antibody [EPR6733] (ab128911)](https://www.abcam.cn/ps/products/128/ab128911/Images/ab128911-142737-anti-acaa2-antibody-epr6733-western-blot.jpg "Western blot - Anti-ACAA2 antibody [EPR6733] (ab128911)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6733] to ACAA2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-ACAA2抗体[EPR6733]
参阅全部 ACAA2 一抗 -
描述
兔单克隆抗体[EPR6733] to ACAA2 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details
不适用于: Flow Cyt or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide within Human ACAA2 aa 350-450. The exact sequence is proprietary.
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阳性对照
- HepG2, 293T, and HeLa whole cell lysate (ab150035); Human colonic carcinoma tissue. IF/ICC: HepG2 cell line.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数（KD）
KD = 2.30 x 10 -12 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6733 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab128911于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 41 kDa.
|
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
1/100 - 1/250.
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 41 kDa. |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
1/100 - 1/250. |
靶标
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功能
Abolishes BNIP3-mediated apoptosis and mitochondrial damage. -
通路
Lipid metabolism; fatty acid metabolism. -
序列相似性
Belongs to the thiolase family. -
细胞定位
Mitochondrion. Colocalizes with BNIP3 in the mitochondria. - Information by UniProt
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数据库链接
- Entrez Gene: 10449 Human
- Entrez Gene: 52538 Mouse
- Entrez Gene: 170465 Rat
- Omim: 604770 Human
- SwissProt: P42765 Human
- SwissProt: Q8BWT1 Mouse
- SwissProt: P13437 Rat
- Unigene: 200136 Human
see all -
别名
- 3 ketoacyl CoA thiolase antibody
- 3 ketoacyl CoA thiolase mitochondrial antibody
- 3-ketoacyl-CoA thiolase, mitochondrial antibody
see all
图片
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Western blot - Anti-ACAA2 antibody [EPR6733] (ab128911)All lanes : Anti-ACAA2 antibody [EPR6733] (ab128911) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 41 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-ACAA2 antibody [EPR6733] (ab128911)](https://www.abcam.cn/ps/products/128/ab128911/Images/ab128911-236061-anti-acaa2-antibody-epr6733-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ACAA2 antibody [EPR6733] (ab128911)ab128911 stained HepG2 cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab128911 at 1/100 dilution) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAA2 antibody [EPR6733] (ab128911)](https://www.abcam.cn/ps/products/128/ab128911/Images/ab128911-142738-anti-acaa2-antibody-epr6733-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAA2 antibody [EPR6733] (ab128911)ab128911 at 1/100 dilution staining ACAA2 in paraffin embedded Human colonic carcinoma tissue by immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-ACAA2 antibody [EPR6733] (ab128911)](https://www.abcam.cn/ps/products/128/ab128911/Images/ab128911-142736-anti-acaa2-antibody-epr6733.jpg)
OI-RD Scanning - Anti-ACAA2 antibody [EPR6733] (ab128911) -
![Anti-ACAA2 antibody [EPR6733] (ab128911)](https://www.abcam.cn/ps/products/128/ab128911/Images/ab128911-5-benefits-of-recombinant-antibodies.png)
Anti-ACAA2 antibody [EPR6733] (ab128911)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab128911 被引用在 4 文献中.
- Sasaki Y et al. A mouse monoclonal antibody against influenza C virus attenuates acetaminophen-induced liver injury in mice. Sci Rep 11:11816 (2021). PubMed: 34083649
- Lu J et al. Multi-Omics Analysis of Fatty Acid Metabolism in Thyroid Carcinoma. Front Oncol 11:737127 (2021). PubMed: 34976793
- Gao K et al. Qishen granules exerts cardioprotective effects on rats with heart failure via regulating fatty acid and glucose metabolism. Chin Med 15:21 (2020). PubMed: 32158496
- Holditch SJ et al. The consequences of increased 4E-BP1 in polycystic kidney disease. Hum Mol Genet 28:4132-4147 (2019). PubMed: 31646342