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Anti-ACAA1/Beta-ketothiolase抗体[2H6BE7] (ab110289)

Submit a review Submit a question References (4)
Western blot - Anti-ACAA1/Beta-ketothiolase antibody [2H6BE7] (ab110289)
  • Immunoprecipitation - Anti-ACAA1/Beta-ketothiolase antibody [2H6BE7] (ab110289)
  • Immunocytochemistry - Anti-ACAA1/Beta-ketothiolase antibody [2H6BE7] (ab110289)
  • Flow Cytometry - Anti-ACAA1/Beta-ketothiolase antibody [2H6BE7] (ab110289)

Key features and details

  • Mouse monoclonal [2H6BE7] to ACAA1/Beta-ketothiolase
  • Suitable for: ICC, Flow Cyt, IP, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

概述

  • 产品名称

    Anti-ACAA1/Beta-ketothiolase抗体[2H6BE7]
    参阅全部 ACAA1/Beta-ketothiolase 一抗

  • 描述

    小鼠单克隆抗体[2H6BE7] to ACAA1/Beta-ketothiolase

  • 宿主

    Mouse

  • 经测试应用

    适用于: ICC, Flow Cyt, IP, WBmore details

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Tissue, cells or virus. This information is considered to be commercially sensitive.

  • 阳性对照

    • HepG2,HL-60 cells, human liver tissue, rat liver tissue and mouse liver tissue.

  • 常规说明

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Product was previously marketed under the MitoSciences sub-brand.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab110289于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC
Use a concentration of 5 µg/ml.

Heat-induced antigen retrieval improves signal
Flow Cyt
Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IP
Use a concentration of 5 µg/ml.
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 44 kDa.
说明
ICC
Use a concentration of 5 µg/ml.

Heat-induced antigen retrieval improves signal
Flow Cyt
Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IP
Use a concentration of 5 µg/ml.
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 44 kDa.

靶标

  • 通路

    Lipid metabolism; fatty acid metabolism.

  • 序列相似性

    Belongs to the thiolase family.

  • 细胞定位

    Peroxisome.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • 3 ketoacyl CoA thiolase peroxisomal antibody
    • 3-ketoacyl-CoA thiolase antibody
    • ACAA antibody
    • ACAA1 antibody
    • Acetyl CoA acyltransferase 1 antibody
    • Acetyl Coenzyme A acyltransferase 1 antibody
    • Acetyl-CoA acyltransferase antibody
    • Acetyl-Coenzyme A acyltransferase 1 (peroxisomal 3 oxoacyl Coenzyme A antibody
    • Beta ketothiolase antibody
    • Beta-ketothiolase antibody
    • Peroxisomal 3 oxoacyl CoA thiolase antibody
    • Peroxisomal 3 oxoacyl Coenzyme A thiolase antibody
    • Peroxisomal 3-oxoacyl-CoA thiolase antibody
    • peroxisomal antibody
    • PTHIO antibody
    • THIK_HUMAN antibody
    • THIO antibody
    see all

图片

  • Western blot - Anti-ACAA1/Beta-ketothiolase antibody [2H6BE7] (ab110289)
    Western blot - Anti-ACAA1/Beta-ketothiolase antibody [2H6BE7] (ab110289)
    All lanes : Anti-ACAA1/Beta-ketothiolase antibody [2H6BE7] (ab110289) at 1 µg/ml

    Lane 1 : Human liver
    Lane 2 : HepG2
    Lane 3 : Rat liver
    Lane 4 : Mouse liver

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 44 kDa

  • Immunoprecipitation - Anti-ACAA1/Beta-ketothiolase antibody [2H6BE7] (ab110289)
    Immunoprecipitation - Anti-ACAA1/Beta-ketothiolase antibody [2H6BE7] (ab110289)

    ACAA1/Beta-ketothiolase was immunoprecipitated using 0.5mg Mouse Liver tissue lysate, 5µg of Mouse monoclonal to ACAA1/Beta-ketothiolase and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, Mouse Liver tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab110289.

    Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.

    Band: 44kDa; ACAA1/Beta-ketothiolase

  • Immunocytochemistry - Anti-ACAA1/Beta-ketothiolase antibody [2H6BE7] (ab110289)
    Immunocytochemistry - Anti-ACAA1/Beta-ketothiolase antibody [2H6BE7] (ab110289)

    ab110289, at 5 µg/mL, staining ACAA1/Beta-ketothiolase in Human HepG2 cells. DAPI was used to stain the cell nuclei (blue).

  • Flow Cytometry - Anti-ACAA1/Beta-ketothiolase antibody [2H6BE7] (ab110289)
    Flow Cytometry - Anti-ACAA1/Beta-ketothiolase antibody [2H6BE7] (ab110289)

    ab110289, at 1 µg/mL, staining ACAA1/Beta-ketothiolase in HL-60 cells (blue) or using an isotype control (red).

文献 (4)

发表研究结果有使用 ab110289？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab110289 被引用在 4 文献中.

  • Uzor NE  et al. Aging lowers PEX5 levels in cortical neurons in male and female mouse brains. Mol Cell Neurosci 107:103536 (2020). PubMed: 32777345
  • Xue Y  et al. Maternal undernutrition induces fetal hepatic lipid metabolism disorder and affects the development of fetal liver in a sheep model. FASEB J 33:9990-10004 (2019). PubMed: 31167079
  • Zhang S  et al. hsa-miR-29c-3p regulates biological function of colorectal cancer by targeting SPARC. Oncotarget 8:104508-104524 (2017). PubMed: 29262657
  • Tomilov A  et al. Shc depletion stimulates brown fat activity in vivo and in vitro. Aging Cell 13:1049-58 (2014). Mouse . PubMed: 25257068

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