AB66037

Anti-AC5抗体

Name: AC5抗体 (ab66037)| Abcam中文官网
Brand: Abcam Limited
SKU: AB66037
Price: 4890 CNY
Availability: InStock

Anti-AC5 antibody

Be the first to review this product! Submit a review

(7 Publications)

Rabbit Polyclonal AC5 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human Adenylate cyclase type 5.

查看别名

Adenylate cyclase type 5, ATP pyrophosphate-lyase 5, Adenylate cyclase type V, Adenylyl cyclase 5, AC5, ADCY5

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-AC5 antibody (AB66037)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-AC5 antibody (AB66037)

ICC/IF image of ab66037 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66037, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Unknown

Western blot - Anti-AC5 antibody (AB66037)

All lanes:

Western blot - Anti-AC5 antibody (ab66037) at 1/500 dilution

Lane 1:

Extracts from COLO205 cells at 30 µg with no immunising peptide

Lane 2:

Extracts from COLO205 cells at 30 µg with immunising peptide

Predicted band size: 139 kDa

Observed band size: 139 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, ICC/IF

applications

免疫原

Synthetic Peptide within Human Adenylate cyclase type 5. The exact immunogen used to generate this antibody is proprietary information.

O95622

特异性

This antibody can cross-react with ADCY6.

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Adenylate Cyclase 5 (AC5) is an enzyme that plays an essential role in catalyzing the conversion of ATP to cyclic AMP (cAMP). AC5 is also known as ADCY5. Its molecular weight is approximately 150 kDa. AC5 is mostly expressed in the brain and heart tissues suggesting its significant role in central and cardiovascular functions.

Biological function summary

AC5 contributes to the regulation of cAMP levels an important secondary messenger in many signaling cascades. AC5 forms part of enzyme complexes that modulate cellular responses through cAMP signaling pathways. Alteration in its activity can directly impact the regulation of neurotransmitter and hormone activity influencing processes such as learning memory and myocardial contractility.

Pathways

AC5 integrates into the cAMP-dependent signaling pathway and the adrenergic signaling pathway. It associates with other proteins like G protein-coupled receptors (GPCRs) and protein kinase A (PKA) that enhance these pathways' functions. Through its role in these pathways AC5 influences diverse physiological responses such as cardiac rhythm and neural transmission.

AC5 has a significant connection to heart failure and movement disorders such as dyskinesia. Heart failure relates to AC5 through its regulation of cardiac contractility where disruption in cAMP signaling can worsen the condition. In movement disorders like dyskinesia AC5’s connection to dopamine receptors alters the sensitivity of pathways in the brain influencing motor control and stability.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Catalyzes the formation of the signaling molecule cAMP in response to G-protein signaling (PubMed : 15385642, PubMed : 24700542, PubMed : 26206488). Mediates signaling downstream of ADRB1 (PubMed : 24700542). Regulates the increase of free cytosolic Ca(2+) in response to increased blood glucose levels and contributes to the regulation of Ca(2+)-dependent insulin secretion (PubMed : 24740569).

See full target information Adenylate cyclase type 5

style

left-column

文献 (7)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40004085

2025

ADCY5 Gene Affects Seasonal Reproduction in Dairy Goats by Regulating Ovarian Granulosa Cells Steroid Hormone Synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Chenbo Shi,Fuhong Zhang,Qiuya He,Jianjun Man,Yuanpan Mu,Jianqing Zhao,Lu Zhu,Juan J Loor,Jun Luo

Theranostics 11:5650-5674 PubMed33897873

2021

Endogenous glutamate determines ferroptosis sensitivity via ADCY10-dependent YAP suppression in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Zhang,Keke Yu,Lifang Ma,Zijun Qian,Xiaoting Tian,Yayou Miao,Yongjie Niu,Xin Xu,Susu Guo,Yueyue Yang,Zhixian Wang,Xiangfei Xue,Chuanjia Gu,Wentao Fang,Jiayuan Sun,Yongchun Yu,Jiayi Wang

eLife 9: PubMed32894219

2020

Novel C1q receptor-mediated signaling controls neural stem cell behavior and neurorepair.

Applications

Unspecified application

Species

Unspecified reactive species

Francisca Benavente,Katja M Piltti,Mitra J Hooshmand,Aileen A Nava,Anita Lakatos,Brianna G Feld,Dana Creasman,Paul D Gershon,Aileen Anderson

Cell death and differentiation 26:1195-1212 PubMed30209302

2018

Caveolin-1 impairs PKA-DRP1-mediated remodelling of ER-mitochondria communication during the early phase of ER stress.

Applications

Unspecified application

Species

Unspecified reactive species

Roberto Bravo-Sagua,Valentina Parra,Carolina Ortiz-Sandoval,Mario Navarro-Marquez,Andrea E Rodríguez,Natalia Diaz-Valdivia,Carlos Sanhueza,Camila Lopez-Crisosto,Nasser Tahbaz,Beverly A Rothermel,Joseph A Hill,Mariana Cifuentes,Thomas Simmen,Andrew F G Quest,Sergio Lavandero

Pflugers Archiv : European journal of physiology 470:1705-1717 PubMed30094477

2018

Adenylyl cyclase 6 is involved in the hyposecretory status of experimental colitis.

Applications

Unspecified application

Species

Unspecified reactive species

Isabel Romero-Calvo,Borja Ocón,Reyes Gámez-Belmonte,Cristina Hernández-Chirlaque,Hugo R de Jonge,Marcel J Bijvelds,Olga Martínez-Augustin,Fermín Sánchez de Medina

Diabetes 63:3009-21 PubMed24740569

2014

ADCY5 couples glucose to insulin secretion in human islets.

Applications

IHC

Species

Unspecified reactive species

David J Hodson,Ryan K Mitchell,Lorella Marselli,Timothy J Pullen,Silvia Gimeno Brias,Francesca Semplici,Katy L Everett,Dermot M F Cooper,Marco Bugliani,Piero Marchetti,Vanessa Lavallard,Domenico Bosco,Lorenzo Piemonti,Paul R Johnson,Stephen J Hughes,Daliang Li,Wen-Hong Li,A M James Shapiro,Guy A Rutter

Journal of cell science 125:176-88 PubMed22250198

2012

Adenylate cyclase 5 coordinates the action of ADP, P2Y1, P2Y13 and ATP-gated P2X7 receptors on axonal elongation.

Applications

Unspecified application

Species

Mouse

Ana del Puerto,Juan-Ignacio Díaz-Hernández,Mónica Tapia,Rosa Gomez-Villafuertes,María José Benitez,Jin Zhang,María Teresa Miras-Portugal,Francisco Wandosell,Miguel Díaz-Hernández,Juan José Garrido

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-AC5抗体

Anti-AC5 antibody

Immunocytochemistry/ Immunofluorescence - Anti-AC5 antibody (AB66037)

Western blot - Anti-AC5 antibody (AB66037)

关键信息

宿主种属

克隆

亚型

不含载体蛋白

反应种属

应用

免疫原

特异性