Anti-AC3抗体(ab125093)
Key features and details
- Rabbit polyclonal to AC3
- Suitable for: WB, ICC/IF, IHC-FrFl
- Reacts with: Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-AC3抗体
参阅全部 AC3 一抗 -
描述
兔多克隆抗体to AC3 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, IHC-FrFlmore details -
种属反应性
与反应: Rat, Human
预测可用于: Mouse, Chinese hamster
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免疫原
Synthetic peptide corresponding to Rat AC3 aa 1100-1200 (C terminal) conjugated to keyhole limpet haemocyanin (Cysteine residue).
Database link: P21932 -
阳性对照
- Rat olfactory epithelium and frontal cortex lysates; Mixed neuron-glial cultures.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Constituent: PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab125093于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/5000 - 1/10000. Detects a band of approximately 160-200 kDa (predicted molecular weight: 129 kDa).
|
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| ICC/IF |
1/500 - 1/1000.
|
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| IHC-FrFl |
1/10000.
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| 说明 |
|---|
|
WB
1/5000 - 1/10000. Detects a band of approximately 160-200 kDa (predicted molecular weight: 129 kDa). |
|
ICC/IF
1/500 - 1/1000. |
|
IHC-FrFl
1/10000. |
靶标
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功能
Mediates odorant detection (possibly) via modulation of intracellular cAMP concentration. -
组织特异性
Expressed in brain, heart, kidney, liver, lung, pancreas, placenta, and skeletal muscle. -
序列相似性
Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.
Contains 2 guanylate cyclase domains. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 109 Human
- Entrez Gene: 104111 Mouse
- Entrez Gene: 64508 Rat
- Omim: 600291 Human
- SwissProt: O60266 Human
- SwissProt: Q8VHH7 Mouse
- SwissProt: P21932 Rat
- Unigene: 727540 Human
see all -
别名
- AC-III antibody
- AC3 antibody
- ADCY3 antibody
see all
图片
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Immunohistochemistry - Free Floating - Anti-AC3 antibody (ab125093)
Free foating immunohistochemistry of rat hippocampus stained with ab125093 at 1/10000 staining AC3 (red). A mouse antibody staining MECP2 (green), DNA is revealed with DAPI (blue).
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Immunocytochemistry/ Immunofluorescence - Anti-AC3 antibody (ab125093)ab125093, at 1/500, staining AC3 in mixed neuron-glial cultures by Immunofluorescence (red). A mouse monoclonal antibody staining of aII-spectrin (green) is also shown.
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Western blot - Anti-AC3 antibody (ab125093)All lanes : Anti-AC3 antibody (ab125093) at 1/5000 dilution
Lane 1 : Rat olfactory epithelium lysate
Lane 2 : Rat frontal cortex lysate
Predicted band size: 129 kDa
实验方案
数据表及文件
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Datasheet download
文献 (14)
ab125093 被引用在 14 文献中.
- Ning K et al. Characterization of Primary Cilia Formation in Human ESC-Derived Retinal Organoids. Stem Cells Int 2023:6494486 (2023). PubMed: 36684387
- Yeo S et al. Primary cilia-mediated regulation of microglial secretion in Alzheimer's disease. Front Mol Biosci 10:1250335 (2023). PubMed: 37942288
- Jang J et al. Abnormal accumulation of extracellular vesicles in hippocampal dystrophic axons and regulation by the primary cilia in Alzheimer's disease. Acta Neuropathol Commun 11:142 (2023). PubMed: 37667395
- Zhang WW et al. Peripheral ablation of type III adenylyl cyclase induces hyperalgesia and eliminates KOR-mediated analgesia in mice. JCI Insight 7:N/A (2022). PubMed: 34914639
- Kowal TJ et al. Distribution of prototypical primary cilia markers in subtypes of retinal ganglion cells. J Comp Neurol 530:2176-2187 (2022). PubMed: 35434813
