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Signal Transduction Protein Phosphorylation Tyrosine Kinases Other

Anti-ABL1 (phospho Y412)抗体(ab4717)

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Western blot - Anti-ABL1 (phospho Y412) antibody (ab4717)

    Key features and details

    • Rabbit polyclonal to ABL1 (phospho Y412)
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

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    概述

    • 产品名称

      Anti-ABL1 (phospho Y412)抗体
      参阅全部 ABL1 一抗
    • 描述

      兔多克隆抗体to ABL1 (phospho Y412)
    • 宿主

      Rabbit
    • 经测试应用

      适用于: WBmore details
    • 种属反应性

      与反应: Human
      预测可用于: Mouse
    • 免疫原

      Synthetic peptide corresponding to Human ABL1 (phospho Y412).

    • 阳性对照

      • Fibroblasts transfected with oncogenic ∆SH3-Abl.
    • 常规说明

      c-Abl is a 140-150 kDa non-receptor protein tyrosine kinase whose precise functions are not known, but roles for Abl in growth factor and integrin signaling, cell cycle regulation, cytoskeletal reorganization, neurogenesis, and responses to DNA damage and oxidative stress have been suggested. c-Abl kinase activity is increased in vivo by diverse physiological stimuli including ionizing radiation, entry into S phase, integrin activation, and platelet-derived growth factor (PDGF) stimulation. c-Abl contains various protein binding domains that appear to enable it to regulate the functions of many proteins by forming complexes, most notably three isoforms of the oncogenic protein Bcr/Abl. c-Abl becomes fully activated by sequential phosphorylation of tyrosines 412 and 245.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • 存储溶液

      pH: 7.30
      Preservative: 0.05% Sodium azide
      Constituents: PBS, 0.1% BSA

      PBS is without Mg2+ and Ca2+ and BSA is IgG and protease free.
    • Concentration information loading...
    • 纯度

      Immunogen affinity purified
    • 纯化说明

      Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively pre-adsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated c-Abl. The final product is generated by affinity chromatography using a c-Abl derived peptide that is phosphorylated at tyrosine 245.
    • Primary antibody说明

      c-Abl is a 140-150 kDa non-receptor protein tyrosine kinase whose precise functions are not known, but roles for Abl in growth factor and integrin signaling, cell cycle regulation, cytoskeletal reorganization, neurogenesis, and responses to DNA damage and oxidative stress have been suggested. c-Abl kinase activity is increased in vivo by diverse physiological stimuli including ionizing radiation, entry into S phase, integrin activation, and platelet-derived growth factor (PDGF) stimulation. c-Abl contains various protein binding domains that appear to enable it to regulate the functions of many proteins by forming complexes, most notably three isoforms of the oncogenic protein Bcr/Abl. c-Abl becomes fully activated by sequential phosphorylation of tyrosines 412 and 245.
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Signal Transduction
      • Protein Phosphorylation
      • Tyrosine Kinases
      • Other
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Cancer susceptibility
      • Proto-oncogenes
      • Cancer
      • Oncoproteins/suppressors
      • Oncoproteins
      • Signal transducers
      • Neuroscience
      • Development

    相关产品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant human ABL1 protein (ab69810)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab4717于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB
    Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 140 kDa.
    说明
    WB
    Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 140 kDa.

    靶标

    • 功能

      Protein kinase that regulates key processes linked to cell growth and survival. Regulates cytoskeleton remodeling during cell differentiation, cell division and cell adhesion. Localizes to dynamic actin structures, and phosphorylates CRK and CRKL, DOK1, and other proteins controlling cytoskeleton dynamics. Regulates DNA repair potentially by activating the proapoptotic pathway when the DNA damage is too severe to be repaired. Phosphorylates PSMA7 that leads to an inhibition of proteasomal activity and cell cycle transition blocks.
    • 组织特异性

      Widely expressed.
    • 疾病相关

      Note=A chromosomal aberration involving ABL1 is a cause of chronic myeloid leukemia. Translocation t(9;22)(q34;q11) with BCR. The translocation produces a BCR-ABL found also in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL).
    • 序列相似性

      Belongs to the protein kinase superfamily. Tyr protein kinase family. ABL subfamily.
      Contains 1 protein kinase domain.
      Contains 1 SH2 domain.
      Contains 1 SH3 domain.
    • 翻译后修饰

      Phosphorylated by PRKDC (By similarity). DNA damage-induced activation of c-Abl requires the function of ATM and Ser-446 phosphorylation (By similarity). Phosphorylation on Thr-735 is required for binding 14-3-3 proteins for cytoplasmic translocation.
      Isoform IB is myristoylated on Gly-2.
    • 细胞定位

      Cytoplasm > cytoskeleton. Nucleus. Sequestered into the cytoplasm through interaction with 14-3-3 proteins and Nucleus membrane. The myristoylated c-ABL protein is reported to be nuclear.
    • Target information above from: UniProt accession P00519 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 25 Human
      • Entrez Gene: 11350 Mouse
      • Omim: 189980 Human
      • SwissProt: P00519 Human
      • SwissProt: P00520 Mouse
      • Unigene: 431048 Human
      • Unigene: 1318 Mouse
      • Unigene: 474779 Mouse
      • 别名

        • Abelson murine leukemia viral oncogene homolog 1 antibody
        • Abelson tyrosine protein kinase 1 antibody
        • Abl 1 antibody
        • ABL antibody
        • ABL proto oncogene 1 non receptor tyrosine kinase antibody
        • ABL1 antibody
        • ABL1_HUMAN antibody
        • bcr/abl antibody
        • bcr/c abl oncogene protein antibody
        • c ABL antibody
        • c abl oncogene 1 non receptor tyrosine kinase antibody
        • c abl oncogene 1 receptor tyrosine kinase antibody
        • c ABL1 antibody
        • JTK7 antibody
        • p150 antibody
        • Proto oncogene tyrosine protein kinase ABL1 antibody
        • Proto-oncogene c-Abl antibody
        • Tyrosine-protein kinase ABL1 antibody
        • v abl Abelson murine leukemia viral oncogene homolog 1 antibody
        • v abl antibody
        see all

      图片

      • Western blot - Anti-ABL1 (phospho Y412) antibody (ab4717)
        Western blot - Anti-ABL1 (phospho Y412) antibody (ab4717)

        Peptide Competition:
        Fibroblasts transfected with oncogenic ∆SH3-Abl were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C, then were incubated with 0.50 µg/mL phospho c-Abl (Tyr 412) antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphotyrosine containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to phospho c-Abl (Tyr 412) blocks the antibody signal, thereby demonstrating the specificity of the antibody.

        Peptide Competition: Fibroblasts transfected with oncogenic ?SH3-Abl were resolved by SDS-PAGE on a 10%

      实验方案

      • Peptide Competition Experiment
      • Western blotting protocol

      Click here to view the general protocols

      数据表及文件

      • Datasheet download

        Download

      文献 (8)

      发表研究结果有使用 ab4717?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab4717 被引用在 8 文献中.

      • Zhou HJ  et al. Caveolae-mediated Tie2 signaling contributes to CCM pathogenesis in a brain endothelial cell-specific Pdcd10-deficient mouse model. Nat Commun 12:504 (2021). PubMed: 33495460
      • Pandolfi L  et al. Loading Imatinib inside targeted nanoparticles to prevent Bronchiolitis Obliterans Syndrome. Sci Rep 10:20726 (2020). PubMed: 33244143
      • McDaniel NK  et al. AXL Mediates Cetuximab and Radiation Resistance Through Tyrosine 821 and the c-ABL Kinase Pathway in Head and Neck Cancer. Clin Cancer Res 26:4349-4359 (2020). PubMed: 32439698
      • Codullo V  et al. Imatinib-loaded gold nanoparticles inhibit proliferation of fibroblasts and macrophages from systemic sclerosis patients and ameliorate experimental bleomycin-induced lung fibrosis. J Control Release 310:198-208 (2019). PubMed: 31430501
      • Latella L  et al. DNA damage signaling mediates the functional antagonism between replicative senescence and terminal muscle differentiation. Genes Dev 31:648-659 (2017). PubMed: 28446595
      • Kim SY  et al. Rescue of platinum-damaged oocytes from programmed cell death through inactivation of the p53 family signaling network. Cell Death Differ 20:987-97 (2013). PubMed: 23598363
      • Furlan A  et al. Abl interconnects oncogenic Met and p53 core pathways in cancer cells. Cell Death Differ 18:1608-16 (2011). PubMed: 21455220
      • Stevens TL  et al. Using bcr-abl to examine mechanisms by which abl kinase regulates morphogenesis in Drosophila. Mol Biol Cell 19:378-93 (2008). WB . PubMed: 17959833

      客户评价及客户问答

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      Question

      Customer would like to know the immunogen sequence.

      Read More

      Abcam community

      Verified customer

      Asked on Jun 02 2004

      Answer

      Thank you for your enquiry. The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human c-Abl 1b that contains tyrosine 412. The immunogen sequence is approximately 10 amino acid residues. The immunogen would have the phosphorylation site in the middle of the sequence with around 5 aa on one side of the site, and 5 aa on the other side of the site. The exact sequence is considered proprietary. If you have any more questions, please contact us again.

      Read More

      Abcam Scientific Support

      回复于 Jun 07 2004

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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