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重组RabMAb

重组Anti-ABI2抗体[EPR3906] (ab108340)

Reviews (2) Submit a question References (1)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABI2 antibody [EPR3906] (ab108340)
  • Immunocytochemistry/ Immunofluorescence - Anti-ABI2 antibody [EPR3906] (ab108340)
  • Anti-ABI2 antibody [EPR3906] (ab108340)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3906] to ABI2
  • Suitable for: ICC/IF, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-ABI2抗体[EPR3906]
    参阅全部 ABI2 一抗

  • 描述

    兔单克隆抗体[EPR3906] to ABI2

  • 宿主

    Rabbit

  • 经测试应用

    适用于: ICC/IF, IHC-Pmore details
    不适用于: Flow Cyt

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • HeLa, Jurkat, K562, SH-SY5Y and HepG2 cell lysates. Paraffin-embedded Human brain tissue.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • 存储溶液

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • 纯度

    Tissue culture supernatant

  • 克隆

    单克隆

  • 克隆编号

    EPR3906

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab108340于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
1/500 - 1/1000.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
说明
ICC/IF
1/500 - 1/1000.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
应用说明
Is unsuitable for Flow Cyt.

靶标

  • 功能

    May act in regulation of cell growth and transformation by interacting with nonreceptor tyrosine kinases ABL1 and/or ABL2. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Regulates ABL1/c-Abl-mediated phosphorylation of MENA.

  • 序列相似性

    Belongs to the ABI family.
    Contains 1 SH3 domain.
    Contains 1 t-SNARE coiled-coil homology domain.

  • 翻译后修饰

    Is a substrate for ABL1.

  • 细胞定位

    Cytoplasm and Cell projection > lamellipodium. Cell projection > filopodium. Cytoplasm > cytoskeleton. Isoform 1 but not isoform 3 is localized to protruding lamellipodia and filopodia tips.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • Abelson interactor 2 antibody
    • ABI 2 antibody
    • Abi-2 antibody
    • ABI2 antibody
    • ABI2_HUMAN antibody
    • ABI2B antibody
    • Abl binding protein 3 antibody
    • Abl interacting protein 1 (SH3 containing protein) antibody
    • Abl interactor 2 antibody
    • Abl interactor protein 2b antibody
    • Abl-binding protein 3 antibody
    • AblBP3 antibody
    • AIP 1 antibody
    • Arg binding protein 1 antibody
    • Arg protein tyrosine kinase binding protein antibody
    • Arg-binding protein 1 antibody
    • ArgBP1 antibody
    • ARGBPIA antibody
    • ArgBPIB antibody
    • OTTHUMP00000163783 antibody
    • OTTHUMP00000163784 antibody
    • OTTHUMP00000206182 antibody
    • OTTHUMP00000206183 antibody
    • OTTHUMP00000206184 antibody
    • OTTHUMP00000206185 antibody
    • SSH3BP2 antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABI2 antibody [EPR3906] (ab108340)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABI2 antibody [EPR3906] (ab108340)

    ab108340, at 1/250, staining ABI2 in paraffin-embedded Human brain tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-ABI2 antibody [EPR3906] (ab108340)
    Immunocytochemistry/ Immunofluorescence - Anti-ABI2 antibody [EPR3906] (ab108340)
    ab108340, at 1/500, staining ABI2 in HeLa cells by Immunofluorescence.
  • Anti-ABI2 antibody [EPR3906] (ab108340)
    Anti-ABI2 antibody [EPR3906] (ab108340)

文献 (1)

发表研究结果有使用 ab108340？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab108340 被引用在 1 文献中.

  • Crawford LJ  et al. Synergistic effects of proteasome inhibitor carfilzomib in combination with tyrosine kinase inhibitors in imatinib-sensitive and -resistant chronic myeloid leukemia models. Oncogenesis 3:e90 (2014). WB ; Human . PubMed: 24590311

客户评价及客户问答

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1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-ABI2 antibody [EPR3906]

 Excellent
Abreviews
abreview image
Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Cell lysate - whole cell (Fibroblasts)
Specification
Fibroblasts
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Aug 14 2013

Immunoprecipitation abreview for Anti-ABI2 antibody [EPR3906]

 Excellent
Abreviews
abreview image
Application
Immunoprecipitation
Immuno-precipitation step
Other - Dynabeads protein A
Sample
Human Cell lysate - whole cell (HEK 293T Cells)
Specification
HEK 293T Cells
Total protein in input
600 µg
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Aug 13 2013

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