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AB247847

重组Anti-Abi-1 (phospho Y435)抗体[EP3954] - BSA and Azide free

Anti-Abi-1 (phospho Y435) antibody [EP3954] - BSA and Azide free

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Rabbit Recombinant Monoclonal Abi-1 phospho Y435 antibody. Carrier free. Suitable for WB and reacts with Human samples.

查看别名

SSH3BP1, ABI1, Abl interactor 1, Abelson interactor 1, Abl-binding protein 4, Eps8 SH3 domain-binding protein, Nap1-binding protein, Spectrin SH3 domain-binding protein 1, e3B1, Abi-1, AblBP4, Eps8-binding protein, Nap1BP

2 Images
Western blot - Anti-Abi-1 (phospho Y435) antibody [EP3954] - BSA and Azide free (AB247847)
  • WB

Lab

Western blot - Anti-Abi-1 (phospho Y435) antibody [EP3954] - BSA and Azide free (AB247847)

This data was developed using ab109395, the same antibody clone in a different buffer formulation.

ab181602 was used a GAPDH loading control

All lanes:

Western blot - Anti-Abi-1 (phospho Y435) antibody [EP3954] (<a href='/products/primary-antibodies/abi-1-phospho-y435-antibody-ep3954-ab109395'>ab109395</a>) at 1/10000 dilution

Lane 1:

Untreated Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate at 15 µg

Lane 2:

Raji treated with 1mM pervanadate for 30mins whole cell lysate at 15 µg

Lane 3:

Raji treated with 1mM pervanadate for 30mins whole cell lysate, then the membrane was incubated with phosphatase at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 55 kDa

false

Exposure time: 7s

Western blot - Anti-Abi-1 (phospho Y435) antibody [EP3954] - BSA and Azide free (AB247847)
  • WB

Unknown

Western blot - Anti-Abi-1 (phospho Y435) antibody [EP3954] - BSA and Azide free (AB247847)

This data was developed using ab109395, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Abi-1 (phospho Y435) antibody [EP3954] - BSA and Azide free (ab247847) at 1/10000 dilution

All lanes:

Jurkat cell lysate, untreated at 10 µg

Predicted band size: 55 kDa

false

不同偶联物与剂型 (1)

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EP3954

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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搭配使用产品

Primary Antibodies

AB109541

Anti-VASP (phospho S156) antibody [EPR1065(2)]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VASP (phospho S156) antibody [EPR1065(2)] (AB109541)

  • 0
  • 0
Primary Antibodies

AB51256

Anti-WSTF antibody [EP1704Y]

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-WSTF antibody [EP1704Y] (AB51256)

  • 4
  • 2
Primary Antibodies

AB109525

Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5453]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5453] (AB109525)

  • 4
  • 1
Primary Antibodies

AB109464

Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887]

RabMAb|Recombinant

Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (AB109464)

  • 0
  • 0
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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产品详情

ab247847 is the carrier-free version of ab109395.

The production method for this product has been changed from hybridoma to recombniant on 16th April 2024

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
Do Not Freeze
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The Abi-1 protein also known as ABI family member 1 is a small adapter protein with a molecular mass of about 55 kDa. It is mainly expressed in brain and hematopoietic tissues. Abi-1 contains several domains allowing it to interact with a variety of signaling and cytoskeletal proteins. Its role as a linker connects various cellular components and facilitates communication between them important for the dynamic regulation of the actin cytoskeleton.
Biological function summary

Abi-1 influences actin polymerization and cell motility by associating with the WAVE complex. This complex plays an important function in mediating signal transduction from small GTPases to the actin cytoskeleton. Abi-1 helps to organize the cytoskeleton to enable cell movements and morphological changes. Moreover Abi-1 is involved in the regulation of signaling pathways linked with cell growth and differentiation. These functions position Abi-1 as an important factor in maintaining normal cellular function.

Pathways

Abi-1 operates inside the WAVE regulatory complex which affects the Rac1 signaling pathway. This pathway is essential for actin polymerization and cellular migration and involves proteins like N-WASP and Arp2/3 complex. Through its interaction with Rac1 and associated proteins Abi-1 greatly affects cytoskeletal reorganization demonstrating its importance in normal cellular movement and immune cell function.

Alterations in Abi-1 have associations with cancer particularly in leukemia. Abi-1 plays a role in oncogenic processes by interacting with proteins like BCR-ABL which is implicated in the pathogenesis of chronic myeloid leukemia. Additionally Abi-1 can contribute to neurological disorders due to its expression in brain tissues but the mechanisms remain less clearly understood. The disorder-associated signaling dynamics make Abi-1 a promising research focus for therapeutic interventions.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

May act in negative regulation of cell growth and transformation by interacting with nonreceptor tyrosine kinases ABL1 and/or ABL2. May play a role in regulation of EGF-induced Erk pathway activation. Involved in cytoskeletal reorganization and EGFR signaling. Together with EPS8 participates in transduction of signals from Ras to Rac. In vitro, a trimeric complex of ABI1, EPS8 and SOS1 exhibits Rac specific guanine nucleotide exchange factor (GEF) activity and ABI1 seems to act as an adapter in the complex. Regulates ABL1/c-Abl-mediated phosphorylation of ENAH. Recruits WASF1 to lamellipodia and there seems to regulate WASF1 protein level. In brain, seems to regulate the dendritic outgrowth and branching as well as to determine the shape and number of synaptic contacts of developing neurons.
See full target information ABI1 pY435
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