重组Anti-Abi-1 (phospho Y435)抗体[EP3954] (ab109395)
![Western blot - Anti-Abi-1 (phospho Y435) antibody [EP3954] (ab109395)](https://www.abcam.cn/ps/products/109/ab109395/Images/ab109395-538392-anti-abi-1-phospho-y435-antibody-ep3954-western-blot-treated-raji-cells-human.jpg "Western blot - Anti-Abi-1 (phospho Y435) antibody [EP3954] (ab109395)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP3954] to Abi-1 (phospho Y435)
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Abi-1 (phospho Y435)抗体[EP3954]
参阅全部 Abi-1 一抗 -
描述
兔单克隆抗体[EP3954] to Abi-1 (phospho Y435) -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: IHC-P -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Raji cell lysate treated with pervanadate.
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常规说明
The production method for this product has been changed from hybridoma to recombniant on 16th April 2024
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP3954 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109395于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/10000. Predicted molecular weight: 55 kDa.
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| 说明 |
|---|
|
WB
1/10000. Predicted molecular weight: 55 kDa. |
应用说明
Is unsuitable for IHC-P.
靶标
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功能
May act in negative regulation of cell growth and transformation by interacting with nonreceptor tyrosine kinases ABL1 and/or ABL2. May play a role in regulation of EGF-induced Erk pathway activation. Involved in cytoskeletal reorganization and EGFR signaling. Together with EPS8 participates in transduction of signals from Ras to Rac. In vitro, a trimeric complex of ABI1, EPS8 and SOS1 exhibits Rac specific guanine nucleotide exchange factor (GEF) activity and ABI1 seems to act as an adapter in the complex. Regulates ABL1/c-Abl-mediated phosphorylation of ENAH. Recruits WASF1 to lamellipodia and there seems to regulate WASF1 protein level. -
组织特异性
Widely expressed, with highest expression in brain. -
疾病相关
Note=A chromosomal aberration involving ABI1 is a cause of acute leukemias. Translocation t(10;11)(p11.2;q23) with MLL. ABI1 isoform 2 was found to be present in acute leukemia MLL-ABI1 fusion transcript. -
序列相似性
Belongs to the ABI family.
Contains 1 SH3 domain.
Contains 1 t-SNARE coiled-coil homology domain. -
结构域
The t-SNARE coiled-coil homology domain is necessary and sufficient for interaction with STX1A. -
翻译后修饰
In vitro substrate for v-Abl (By similarity). Phosphorylated on tyrosine residues after serum stimulation or induction by v-Abl. -
细胞定位
Cytoplasm. Nucleus. Cell projection > lamellipodium. Cell projection > filopodium. Cell projection > growth cone. Cell junction > synapse > synaptosome. Cytoplasm > cytoskeleton. Localized to protruding lamellipodia and filopodia tips. Also localized to neuronal growth cones and synaptosomes. - Information by UniProt
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数据库链接
- Entrez Gene: 10006 Human
- Omim: 603050 Human
- SwissProt: Q8IZP0 Human
- Unigene: 508148 Human
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别名
- Abelson interactor 1 antibody
- Abi-1 antibody
- Abi1 antibody
see all
图片
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Western blot - Anti-Abi-1 (phospho Y435) antibody [EP3954] (ab109395)All lanes : Anti-Abi-1 (phospho Y435) antibody [EP3954] (ab109395) at 1/10000 dilution
Lane 1 : Untreated Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lane 2 : Raji treated with 1mM pervanadate for 30mins whole cell lysate
Lane 3 : Raji treated with 1mM pervanadate for 30mins whole cell lysate, then the membrane was incubated with phosphatase
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 55 kDa
Exposure time: 7 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
ab181602 was used a GAPDH loading control
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![Anti-Abi-1 (phospho Y435) antibody [EP3954] (ab109395)](https://www.abcam.cn/ps/products/109/ab109395/Images/ab109395-2-benefits-of-recombinant-antibodies.png)
Anti-Abi-1 (phospho Y435) antibody [EP3954] (ab109395)
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab109395 尚未被引用在任何文献中。