AB109395

重组Anti-Abi-1 (phospho Y435)抗体[EP3954]

Anti-Abi-1 (phospho Y435) antibody [EP3954]

RabMAb
Recombinant
了解详情

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(1 Publication)

Rabbit Recombinant Monoclonal Abi-1 phospho Y435 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

查看别名

SSH3BP1, ABI1, Abl interactor 1, Abelson interactor 1, Abl-binding protein 4, Eps8 SH3 domain-binding protein, Nap1-binding protein, Spectrin SH3 domain-binding protein 1, e3B1, Abi-1, AblBP4, Eps8-binding protein, Nap1BP

1 Images

Lab

Western blot - Anti-Abi-1 (phospho Y435) antibody [EP3954] (AB109395)

ab181602 was used a GAPDH loading control

All lanes:

Western blot - Anti-Abi-1 (phospho Y435) antibody [EP3954] (ab109395) at 1/10000 dilution

Lane 1:

Untreated Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate at 15 µg

Lane 2:

Raji treated with 1mM pervanadate for 30mins whole cell lysate at 15 µg

Lane 3:

Raji treated with 1mM pervanadate for 30mins whole cell lysate, then the membrane was incubated with phosphatase at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 55 kDa

false

Exposure time: 7s

不同偶联物与剂型 (1)

Carrier free

Anti-Abi-1 (phospho Y435) antibody [EP3954] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EP3954

亚型

IgG

不含载体蛋白

反应种属

Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }

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产品详情

The production method for this product has been changed from hybridoma to recombniant on 16th April 2024

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Conditional Ambient

储存信息

Stable for 12 months at -20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The Abi-1 protein also known as ABI family member 1 is a small adapter protein with a molecular mass of about 55 kDa. It is mainly expressed in brain and hematopoietic tissues. Abi-1 contains several domains allowing it to interact with a variety of signaling and cytoskeletal proteins. Its role as a linker connects various cellular components and facilitates communication between them important for the dynamic regulation of the actin cytoskeleton.

Biological function summary

Abi-1 influences actin polymerization and cell motility by associating with the WAVE complex. This complex plays an important function in mediating signal transduction from small GTPases to the actin cytoskeleton. Abi-1 helps to organize the cytoskeleton to enable cell movements and morphological changes. Moreover Abi-1 is involved in the regulation of signaling pathways linked with cell growth and differentiation. These functions position Abi-1 as an important factor in maintaining normal cellular function.

Pathways

Abi-1 operates inside the WAVE regulatory complex which affects the Rac1 signaling pathway. This pathway is essential for actin polymerization and cellular migration and involves proteins like N-WASP and Arp2/3 complex. Through its interaction with Rac1 and associated proteins Abi-1 greatly affects cytoskeletal reorganization demonstrating its importance in normal cellular movement and immune cell function.

Alterations in Abi-1 have associations with cancer particularly in leukemia. Abi-1 plays a role in oncogenic processes by interacting with proteins like BCR-ABL which is implicated in the pathogenesis of chronic myeloid leukemia. Additionally Abi-1 can contribute to neurological disorders due to its expression in brain tissues but the mechanisms remain less clearly understood. The disorder-associated signaling dynamics make Abi-1 a promising research focus for therapeutic interventions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

May act in negative regulation of cell growth and transformation by interacting with nonreceptor tyrosine kinases ABL1 and/or ABL2. May play a role in regulation of EGF-induced Erk pathway activation. Involved in cytoskeletal reorganization and EGFR signaling. Together with EPS8 participates in transduction of signals from Ras to Rac. In vitro, a trimeric complex of ABI1, EPS8 and SOS1 exhibits Rac specific guanine nucleotide exchange factor (GEF) activity and ABI1 seems to act as an adapter in the complex. Regulates ABL1/c-Abl-mediated phosphorylation of ENAH. Recruits WASF1 to lamellipodia and there seems to regulate WASF1 protein level. In brain, seems to regulate the dendritic outgrowth and branching as well as to determine the shape and number of synaptic contacts of developing neurons.

See full target information ABI1 pY435

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 135: PubMed40794452

2025

Epigenetic targeting of PGBD5-dependent DNA damage in SMARCB1-deficient sarcomas.

Applications

Unspecified application

Species

Unspecified reactive species

Yaniv Kazansky,Helen S Mueller,Daniel Cameron,Phillip Demarest,Nadia Zaffaroni,Noemi Arrighetti,Valentina Zuco,Prabhjot S Mundi,Yasumichi Kuwahara,Romel Somwar,Rui Qu,Andrea Califano,Elisa de Stanchina,Filemon S Dela Cruz,Andrew L Kung,Mrinal M Gounder,Alex Kentsis

View all publications

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-Abi-1 (phospho Y435)抗体[EP3954]