Anti-Abi-1抗体(ab72318)
Key features and details
- Rabbit polyclonal to Abi-1
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Abi-1抗体
参阅全部 Abi-1 一抗 -
描述
兔多克隆抗体to Abi-1 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
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免疫原
Synthetic peptide within Human Abi-1 aa 325-375. The exact sequence is proprietary.
Database link: NP_005461.2 -
阳性对照
- Whole cell lysates from HeLa and 293T cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab72318于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
1/2000 - 1/10000. Detects a band of approximately 55, 60 kDa (predicted molecular weight: 55 kDa).
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| IP |
Use at 2-5 µg/mg of lysate.
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| 说明 |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
1/2000 - 1/10000. Detects a band of approximately 55, 60 kDa (predicted molecular weight: 55 kDa). |
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IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
May act in negative regulation of cell growth and transformation by interacting with nonreceptor tyrosine kinases ABL1 and/or ABL2. May play a role in regulation of EGF-induced Erk pathway activation. Involved in cytoskeletal reorganization and EGFR signaling. Together with EPS8 participates in transduction of signals from Ras to Rac. In vitro, a trimeric complex of ABI1, EPS8 and SOS1 exhibits Rac specific guanine nucleotide exchange factor (GEF) activity and ABI1 seems to act as an adapter in the complex. Regulates ABL1/c-Abl-mediated phosphorylation of ENAH. Recruits WASF1 to lamellipodia and there seems to regulate WASF1 protein level. -
组织特异性
Widely expressed, with highest expression in brain. -
疾病相关
Note=A chromosomal aberration involving ABI1 is a cause of acute leukemias. Translocation t(10;11)(p11.2;q23) with MLL. ABI1 isoform 2 was found to be present in acute leukemia MLL-ABI1 fusion transcript. -
序列相似性
Belongs to the ABI family.
Contains 1 SH3 domain.
Contains 1 t-SNARE coiled-coil homology domain. -
结构域
The t-SNARE coiled-coil homology domain is necessary and sufficient for interaction with STX1A. -
翻译后修饰
In vitro substrate for v-Abl (By similarity). Phosphorylated on tyrosine residues after serum stimulation or induction by v-Abl. -
细胞定位
Cytoplasm. Nucleus. Cell projection > lamellipodium. Cell projection > filopodium. Cell projection > growth cone. Cell junction > synapse > synaptosome. Cytoplasm > cytoskeleton. Localized to protruding lamellipodia and filopodia tips. Also localized to neuronal growth cones and synaptosomes. - Information by UniProt
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数据库链接
- Entrez Gene: 10006 Human
- Entrez Gene: 11308 Mouse
- Entrez Gene: 100738420 Pig
- Entrez Gene: 79249 Rat
- Omim: 603050 Human
- SwissProt: Q8IZP0 Human
- SwissProt: Q8CBW3 Mouse
- SwissProt: Q9QZM5 Rat
see all -
别名
- Abelson interactor 1 antibody
- Abi-1 antibody
- Abi1 antibody
see all
图片
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Western blot - Anti-Abi-1 antibody (ab72318)All lanes : Anti-Abi-1 antibody (ab72318) at 0.04 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Predicted band size: 55 kDa
Observed band size: 55,60 kDa why is the actual band size different from the predicted?
Additional bands at: 28 kDa, 90 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Abi-1 antibody (ab72318)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling Abi-1 with ab72318 at 1/200 (1µg/ml). Detection: DAB.
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Immunoprecipitation - Anti-Abi-1 antibody (ab72318)Detection of Human Abi-1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Lane 1: ab72317 used for IP at 3 µg/mg lysate. Lane 2: Abi-1 immunoprecipitated with an other antibody. Lane 3: ab72318 at 3 µg/mg lysate used for IP of Abi-1, which recognize downstream epitopes. Lane 4: control Ig IP For blotting immunoprecipitated Abi-1, ab72318 was used at 1 µg/ml.
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Immunoprecipitation - Anti-Abi-1 antibody (ab72318)Detection of Abi-1 by Immunoprecipitation from Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab72318 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent WB detection. Lane 2 represents control IgG IP.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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