AB183739

重组Anti-Abhd5/CGI-58抗体[EPR12621]

Name: Abhd5/CGI-58抗体[EPR12621] (ab183739)| Abcam中文官网
Brand: Abcam Limited
SKU: AB183739
Price: 1478 CNY
Availability: InStock
Rating: 5 (2 reviews)

Anti-Abhd5/CGI-58 antibody [EPR12621]

RabMAb
Recombinant
20ul selling size
了解详情

(2 Reviews)

(24 Publications)

Rabbit Recombinant Monoclonal Abhd5/CGI-58 antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 24 publications.

查看别名

NCIE2, CGI-58, ABHD5, 1-acylglycerol-3-phosphate O-acyltransferase ABHD5, Abhydrolase domain-containing protein 5, Lipid droplet-binding protein CGI-58

4 Images

Flow Cytometry (Intracellular) - Anti-Abhd5/CGI-58 antibody [EPR12621] (AB183739)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Abhd5/CGI-58 antibody [EPR12621] (AB183739)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HepG2 cells labeling Abhd5/CGI-58 with ab183739 at 1/200 dilution. Goat anti-rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody. Rabbit monoclonal IgG was used as the isotype control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Abhd5/CGI-58 antibody [EPR12621] (AB183739)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Abhd5/CGI-58 antibody [EPR12621] (AB183739)

Immunohistochemical analysis of paraffin-embedded Human lipomyoma tissue labeling Abhd5/CGI-58 with ab183739 at 1/2000 dilution. The slide is counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-Abhd5/CGI-58 antibody [EPR12621] (AB183739)

PMID : 28211464, PMID : 19801371, PMID : 18606822, PMID : 18832586.

All lanes:

Western blot - Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

U87-MG cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 39 kDa

Observed band size: 45 kDa

false

Supplier Data

Western blot - Anti-Abhd5/CGI-58 antibody [EPR12621] (AB183739)

PMID : 28211464, PMID : 19801371, PMID : 18606822, PMID : 18832586.

All lanes:

Western blot - Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739) at 1/1000 dilution

All lanes:

Human fetal liver lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 39 kDa

Observed band size: 45 kDa

false

不同偶联物与剂型 (10)

775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-Abhd5/CGI-58 antibody [EPR12621]
Carrier free

Anti-Abhd5/CGI-58 antibody [EPR12621] - BSA and Azide free
660 APC

APC Anti-Abhd5 / CGI-58 antibody [EPR12621]
HRP

HRP Anti-Abhd5 / CGI-58 antibody [EPR12621]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Abhd5 / CGI-58 antibody [EPR12621]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Abhd5 / CGI-58 antibody [EPR12621]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-Abhd5 / CGI-58 antibody [EPR12621]
578 PE

PE Anti-Abhd5 / CGI-58 antibody [EPR12621]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Abhd5 / CGI-58 antibody [EPR12621]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Abhd5 / CGI-58 antibody [EPR12621]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR12621

亚型

IgG

不含载体蛋白

反应种属

Human

应用

Flow Cyt (Intra), IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/200", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Abhd5 also known as CGI-58 is a protein with significant importance in lipid metabolism. It has a molecular weight of approximately 39 kDa and is ubiquitously expressed in tissues throughout the body including adipose tissue and liver. This protein associates with lipid droplets within cells serving a role important for lipid handling and signaling. Abhd5 stands for the alpha/beta hydrolase domain-containing protein 5 indicative of its structure and enzymatic functions.

Biological function summary

The Abhd5/CGI-58 protein acts as a coactivator of adipose triglyceride lipase (ATGL) enhancing its role in triglyceride hydrolysis. This support is vital for energy release from triglycerides in fat cells. Abhd5 does not function alone; it exists within a protein complex where it modulates ATGL's catalytic activity. Furthermore Abhd5 is implicated in broader lipid signaling and metabolic control influencing various cellular responses through its roles within these complexes.

Pathways

Abhd5/CGI-58 is central to the lipid metabolism pathway particularly in the breakdown of triglycerides. By activating ATGL Abhd5 influences the lipolytic pathway which is important for mobilizing stored fats for energy use. In addition to its role with ATGL another protein perilipin regulates this pathway by interacting with lipid droplet-associated proteins including Abhd5 to control access to lipids. The protein influences the signaling crosstalk within metabolic pathways coordinating metabolic responses within the cell.

The Abhd5/CGI-58 protein has ties to conditions like lipodystrophy and Chanarin-Dorfman syndrome. Deficiencies or mutations in the CGI-58 protein can impair lipid metabolism leading to abnormal fat distribution and storage. In these diseases the lack of Abhd5 activity affects the interactions with proteins like ATGL which is responsible for triglyceride breakdown. Research continues to explore the relationship between Abhd5 dysfunction and these metabolic disorders aiming to clarify its role in the etiology of such conditions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Coenzyme A-dependent lysophosphatidic acid acyltransferase that catalyzes the transfer of an acyl group on a lysophosphatidic acid (PubMed : 18606822). Functions preferentially with 1-oleoyl-lysophosphatidic acid followed by 1-palmitoyl-lysophosphatidic acid, 1-stearoyl-lysophosphatidic acid and 1-arachidonoyl-lysophosphatidic acid as lipid acceptor. Functions preferentially with arachidonoyl-CoA followed by oleoyl-CoA as acyl group donors (By similarity). Functions in phosphatidic acid biosynthesis (PubMed : 18606822). May regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2 (PubMed : 16679289). Involved in keratinocyte differentiation (PubMed : 18832586). Regulates lipid droplet fusion (By similarity).

See full target information ABHD5

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文献 (24)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:9475 PubMed39488519

2024

CLSTN3B promotes lipid droplet maturation and lipid storage in mouse adipocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Chuanhai Zhang,Mengchen Ye,Kamran Melikov,Dengbao Yang,Goncalo Dias do Vale,Jeffrey McDonald,Kaitlyn Eckert,Mei-Jung Lin,Xing Zeng

JCI insight 9: PubMed39316445

2024

Extracellular vesicle transfer of miR-1 to adipose tissue modifies lipolytic pathways following resistance exercise.

Applications

Unspecified application

Species

Unspecified reactive species

Benjamin I Burke,Ahmed Ismaeel,Douglas E Long,Lauren A Depa,Peyton T Coburn,Jensen Goh,Tolulope P Saliu,Bonnie J Walton,Ivan J Vechetti,Bailey D Peck,Taylor R Valentino,C Brooks Mobley,Hasiyet Memetimin,Dandan Wang,Brian S Finlin,Philip A Kern,Charlotte A Peterson,John J McCarthy,Yuan Wen

International journal of pharmaceutics: X 6:100195 PubMed37448985

2023

Sustained release of therapeutic gene by injectable hydrogel for hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Shuangta Xu,Jianya Cai,Hongwei Cheng,Wei Wang

Cell reports 42:112091 PubMed36763501

2023

PNPLA2 mobilizes retinyl esters from retinosomes and promotes the generation of 11-cis-retinal in the visual cycle.

Applications

Unspecified application

Species

Unspecified reactive species

Miwa Hara,Wenjing Wu,Volha V Malechka,Yusuke Takahashi,Jian-Xing Ma,Gennadiy Moiseyev

Aging 14:10171-10216 PubMed36585927

2022

The tumor microenvironment in gastrointestinal adenocarcinomas revealed a prognostic and immunotherapeutic biomarker.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Zhang,Lu Li,Feifei Chu,Lei Zhang,Li Zhang,Huili Wu,Kunkun Li

Nature 613:160-168 PubMed36477540

2022

CLSTN3β enforces adipocyte multilocularity to facilitate lipid utilization.

Applications

Unspecified application

Species

Unspecified reactive species

Kevin Qian,Marcus J Tol,Jin Wu,Lauren F Uchiyama,Xu Xiao,Liujuan Cui,Alexander H Bedard,Thomas A Weston,Pradeep S Rajendran,Laurent Vergnes,Yuta Shimanaka,Yesheng Yin,Yasaman Jami-Alahmadi,Whitaker Cohn,Bryce T Bajar,Chia-Ho Lin,Benita Jin,Laura A DeNardo,Douglas L Black,Julian P Whitelegge,James A Wohlschlegel,Karen Reue,Kalyanam Shivkumar,Feng-Jung Chen,Stephen G Young,Peng Li,Peter Tontonoz

Nature communications 13:6577 PubMed36323699

2022

Phosphorylation of 17β-hydroxysteroid dehydrogenase 13 at serine 33 attenuates nonalcoholic fatty liver disease in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Wen Su,Sijin Wu,Yongliang Yang,Yanlin Guo,Haibo Zhang,Jie Su,Lei Chen,Zhuo Mao,Rongfeng Lan,Rong Cao,Chunjiong Wang,Hu Xu,Cong Zhang,Sha Li,Min Gao,Xiaocong Chen,Zhiyou Zheng,Bing Wang,Yi'ao Liu,Zuojun Liu,Zimei Wang,Baohua Liu,Xinmin Fan,Xiaoyan Zhang,Youfei Guan

Journal of clinical periodontology 49:1192-1202 PubMed35924763

2022

Lipidomic analysis reveals altered lipid profiles of gingival tissues with periodontitis.

Applications

Unspecified application

Species

Unspecified reactive species

Min Zhou,Chen Li,Xue Han,Bohan Yu,Xiang-Zhen Yan,Yan Zhang,Xiao-Juan Yang

Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme 47:992-1004 PubMed35816737

2022

One week of overeating upregulates angiogenic and lipolytic gene expression in human subcutaneous adipose tissue from exercise trained and untrained adults.

Applications

Unspecified application

Species

Unspecified reactive species

Alison C Ludzki,Emily M Krueger,Jenna B Gillen,Natalie M Taylor,Darby O Middlebrook,Toree C Baldwin,Konstantinos C Karabetsos,Michael W Schleh,Jeffrey F Horowitz

The Journal of physiology 600:2127-2146 PubMed35249225

2022

Exercise training remodels subcutaneous adipose tissue in adults with obesity even without weight loss.

Applications

Unspecified application

Species

Unspecified reactive species

Cheehoon Ahn,Benjamin J Ryan,Michael W Schleh,Pallavi Varshney,Alison C Ludzki,Jenna B Gillen,Douglas W Van Pelt,Lisa M Pitchford,Suzette M Howton,Thomas Rode,Scott L Hummel,Charles F Burant,Jonathan P Little,Jeffrey F Horowitz

View all publications

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重组Anti-Abhd5/CGI-58抗体[EPR12621]