重组Anti-ABCG1抗体[EP1366Y] (ab52617)
![Western blot - Anti-ABCG1 antibody [EP1366Y] (ab52617)](https://www.abcam.cn/ps/products/52/ab52617/Images/ab52617-270913-anti-abcg1-antibody-ep1366y-western-blot.jpg "Western blot - Anti-ABCG1 antibody [EP1366Y] (ab52617)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1366Y] to ABCG1
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Cow, Human
Related conjugates and formulations
概述
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产品名称
Anti-ABCG1抗体[EP1366Y]
参阅全部 ABCG1 一抗 -
描述
兔单克隆抗体[EP1366Y] to ABCG1 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WBmore details
不适用于: IHC-P -
种属反应性
与反应: Mouse, Rat, Cow, Human -
免疫原
Synthetic peptide within Human ABCG1 aa 150-250. The exact sequence is proprietary.
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阳性对照
- ICC/IF: HUVEC cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1366Y -
同种型
IgG -
研究领域
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab52617于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (1) |
1/100.
|
| WB | (3) |
1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 75 kDa).
|
| 说明 |
|---|
|
ICC/IF
1/100. |
|
WB
1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 75 kDa). |
靶标
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功能
Transporter involved in macrophage lipid homeostasis. Is an active component of the macrophage lipid export complex. Could also be involved in intracellular lipid transport processes. The role in cellular lipid homeostasis may not be limited to macrophages. -
组织特异性
Expressed in several tissues. Expressed in macrophages; expression is increased in macrophages from patients with Tangier disease. -
序列相似性
Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
Contains 1 ABC transmembrane type-2 domain.
Contains 1 ABC transporter domain. -
细胞定位
Endoplasmic reticulum membrane. Golgi apparatus membrane. Predominantly localized in the intracellular compartments mainly associated with the endoplasmic reticulum (ER) and Golgi membranes. - Information by UniProt
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数据库链接
- Entrez Gene: 510745 Cow
- Entrez Gene: 9619 Human
- Entrez Gene: 11307 Mouse
- Entrez Gene: 85264 Rat
- Omim: 603076 Human
- SwissProt: P45844 Human
- SwissProt: Q64343 Mouse
- Unigene: 124649 Human
see all -
别名
- ABC transporter 8 antibody
- ABC8 antibody
- ABCG1 antibody
see all
图片
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Western blot - Anti-ABCG1 antibody [EP1366Y] (ab52617)All lanes : Anti-ABCG1 antibody [EP1366Y] (ab52617) at 1/1000 dilution ((purified))
Lane 1 : Rat kidney lysate
Lane 2 : Rat liver lysate
Lane 3 : MDBK (Bovine kidney cell line) whole cell lysate
Lane 4 : HUVEC (Human umbilical vein endothelial cell line) whole cell lysate
Lane 5 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 6 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 75 kDaBlocking and diluting buffer: 5% NFDM /TBST
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![Immunocytochemistry/ Immunofluorescence - Anti-ABCG1 antibody [EP1366Y] (ab52617)](https://www.abcam.cn/ps/products/52/ab52617/Images/ab52617-270915-anti-abcg1-antibody-ep1366y-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ABCG1 antibody [EP1366Y] (ab52617)ab52617 staining ABCG1 in HUVEC (Human umbilical vein endothelial cell line) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. ab195889 was used as a counterstain for primary antibody ab52617 at 1/200. DAPI was used as a nuclear counterstain and PBS as a negative control.
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![Western blot - Anti-ABCG1 antibody [EP1366Y] (ab52617)](https://www.abcam.cn/ps/products/52/ab52617/Images/ab52617-505655-anti-abcg1-antibody-ep1366y-wetern-blot-c-myc-ddk-tagged-full-length-human-abcg1-recombinant-protein.png)
Western blot - Anti-ABCG1 antibody [EP1366Y] (ab52617)Lane 1 : Anti-ABCG1 antibody [EP1366Y] (ab52617) at 1/1000 dilution
Lane 2 : Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (ab205606) at 1/1000 dilution
All lanes : C-Myc/DDK-tagged full length human ABCG1 recombinant protein 10 ng
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 75 kDa
Observed band size: 75 kDa
Exposure time: 180 secondsBlocking/Dilution buffer: 5% NFDM/TBST
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![Western blot - Anti-ABCG1 antibody [EP1366Y] (ab52617)](https://www.abcam.cn/ps/products/52/ab52617/Images/ab52617-64963-anti-abcg1-antibody-ep1366y-western-blot.jpg)
Western blot - Anti-ABCG1 antibody [EP1366Y] (ab52617)Anti-ABCG1 antibody [EP1366Y] (ab52617) at 1/2000 dilution + HUVEC cell lysate at 10 µg
Secondary
Goat anti-rabbit HRP antiibody at 1/2000 dilution
Predicted band size: 75 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted? -
![Anti-ABCG1 antibody [EP1366Y] (ab52617)](https://www.abcam.cn/ps/products/52/ab52617/Images/ab52617-5-benefits-of-recombinant-antibodies.png)
Anti-ABCG1 antibody [EP1366Y] (ab52617)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (76)
ab52617 被引用在 76 文献中.
- Lu X et al. MicroRNA-320b Modulates Cholesterol Efflux and Atherosclerosis. J Atheroscler Thromb 29:200-220 (2022). PubMed: 33536383
- Gui M et al. Modified Yuejuwan Inhibited Cholesterol Accumulation and Inflammation in THP-1 Macrophage-Derived Foam Cells by Inhibiting the Activity of the TRIM37/TRAF2/NF-κB Pathway. Evid Based Complement Alternat Med 2022:6400517 (2022). PubMed: 35310029
- Hu J et al. A reduction of Syndecan-4 in macrophages promotes atherosclerosis by aggravating the proinflammatory capacity of macrophages. J Transl Med 20:319 (2022). PubMed: 35842658
- Wang Y et al. 27-Hydroxycholesterol-Induced Dysregulation of Cholesterol Metabolism Impairs Learning and Memory Ability in ApoE ε4 Transgenic Mice. Int J Mol Sci 23:N/A (2022). PubMed: 36232940
- Tan Z et al. KLF2 alleviates endothelial cell injury and inhibits the formation of THP‑1 macrophage‑derived foam cells by activating Nrf2 and enhancing autophagy. Exp Ther Med 24:737 (2022). PubMed: 36478888