重组Anti-ABCB4抗体[EPR28774-92] (ab317573)
![Western blot - Anti-ABCB4 antibody [EPR28774-92] (ab317573)](https://www.abcam.cn/ps/products/317/ab317573/Images/ab317573-542231-anti-abcb4-antibody-epr28774-92-western-blot-untreated-mouse-liver-tissue-lysate-20-ug.jpg "Western blot - Anti-ABCB4 antibody [EPR28774-92] (ab317573)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR28774-92] to ABCB4
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-ABCB4抗体[EPR28774-92]
参阅全部 ABCB4 一抗 -
描述
兔单克隆抗体[EPR28774-92] to ABCB4 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: ICC/IF,IHC-P or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Untreated Mouse liver, Mouse liver treated with Peptide:N-glycosidase F, Mouse liver non-membrane fraction, Mouse liver membrane fraction, Mouse liver, Rat liver, Human liver cancer, Human liver, McA-RH7777 and 293T cells transfected with a Human ABCB4 expression vector containing a His-tag, whole cell lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR28774-92 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab317573于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 142 kDa.
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| 说明 |
|---|
|
WB
1/1000. Predicted molecular weight: 142 kDa. |
靶标
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功能
Mediates ATP-dependent export of organic anions and drugs from the cytoplasm. Hydrolyzes ATP with low efficiency. Human MDR3 is not capable of conferring drug resistance. Mediates the translocation of phosphatidylcholine across the canalicular membrane of the hepatocyte. -
疾病相关
Defects in ABCB4 are the cause of progressive familial intrahepatic cholestasis type 3 (PFIC3) [MIM:602347]. PFIC3 is an autosomal recessive liver disorder presenting with early onset cholestasis that progresses to cirrhosis and liver failure before adulthood. It is characterized by elevated serum gamma-glutamyltransferase levels.
Defects in ABCB4 are a cause of intrahepatic cholestasis of pregnancy (ICP) [MIM:147480]; also known as obstetric cholestasis. ICP is a multifactorial liver disorder of pregnancy. It presents during the second or, more commonly, the third trimestre of pregnancy with intense pruritus which becomes more severe with advancing gestation and cholestasis. Cholestasis results from abnormal biliary transport from the liver into the small intestine. ICP causes fetal distress, spontaneous premature delivery and intrauterine death. ICP patients have spontaneous and progressive disappearance of cholestasis after delivery.
Defects in ABCB4 are a cause of gallbladder disease type 1 (GBD1) [MIM:600803]. It is one of the major digestive diseases. Gallstones composed of cholesterol (cholelithiasis) are the common manifestations in western countries. Most people with gallstones, however, remain asymptomatic through their lifetimes. -
序列相似性
Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
Contains 2 ABC transmembrane type-1 domains.
Contains 2 ABC transporter domains. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 5244 Human
- Entrez Gene: 18671 Mouse
- Entrez Gene: 24891 Rat
- Omim: 171060 Human
- SwissProt: P21439 Human
- SwissProt: P21447 Mouse
- SwissProt: Q08201 Rat
- Unigene: 654403 Human
see all -
别名
- ABC 21 antibody
- ABC B4 antibody
- ABC21 antibody
see all
图片
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Western blot - Anti-ABCB4 antibody [EPR28774-92] (ab317573)All lanes : Anti-ABCB4 antibody [EPR28774-92] (ab317573) at 1/1000 dilution
Lane 1 : Untreated Mouse liver tissue lysate
Lane 2 : Mouse liver tissue lysate treated with Peptide:N-glycosidase F (PNGase F)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 142 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?
Additional bands at: 150 kDa (possible glycosylated form)
Exposure time: 92 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
ABCB4 is a glycoprotein of approximately 150 kDa and detected as a 140-kDa band after treated with Peptide:N-glycosidase F (PNGase F).
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 28220208, PMID: 24806754).
The identity of the bands at 37 kDa and 60 kDa are unknown.
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
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![Western blot - Anti-ABCB4 antibody [EPR28774-92] (ab317573)](https://www.abcam.cn/ps/products/317/ab317573/Images/ab317573-542232-anti-abcb4-antibody-epr28774-92-western-blot-mouse-liver-tissue-lysate-non-membrane-fraction-20-ug.jpg)
Western blot - Anti-ABCB4 antibody [EPR28774-92] (ab317573)All lanes : Anti-ABCB4 antibody [EPR28774-92] (ab317573) at 1/1000 dilution
Lane 1 : Mouse liver tissue lysate non-membrane fraction
Lane 2 : Mouse liver tissue lysate membrane fraction
Lane 3 : Mouse liver tissue lysate
Lane 4 : Mouse spleen tissue lysate
Lane 5 : Mouse skeletal muscle tissue lysate
Lane 6 : Rat liver tissue lysate
Lane 7 : Rat spleen tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 142 kDa
Observed band size: 140-150 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 28220208).
Low expression: spleen, skeletal muscle (PMID: 7734012).
The identity of the bands at 37 kDa and 60 kDa are unknown.
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
In Western blot, Anti-E Cadherin antibody [EPR16845-108] (ab181296) staining at 1/1000 dilution.
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![Western blot - Anti-ABCB4 antibody [EPR28774-92] (ab317573)](https://www.abcam.cn/ps/products/317/ab317573/Images/ab317573-542233-anti-abcb4-antibody-epr28774-92-western-blot-human-liver-cancer-tissue-lysate-50-ug.jpg)
Western blot - Anti-ABCB4 antibody [EPR28774-92] (ab317573)All lanes : Anti-ABCB4 antibody [EPR28774-92] (ab317573) at 1/1000 dilution
Lane 1 : Human liver cancer tissue lysate
Lane 2 : Human liver tissue lysate
Lane 3 : Human brainstem tissue lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 142 kDa
Observed band size: 140-250 kDa why is the actual band size different from the predicted?
Exposure time: 70 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 28220208).
Low expression: brain (PMID: 7734012).
The identity of the bands at 37 kDa and 60 kDa are unknown.
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
-
![Western blot - Anti-ABCB4 antibody [EPR28774-92] (ab317573)](https://www.abcam.cn/ps/products/317/ab317573/Images/ab317573-542234-anti-abcb4-antibody-epr28774-92-western-blot-mca-rh7777-rat-liver-epithelial-cell-whole-cell-lysate-20-ug.jpg)
Western blot - Anti-ABCB4 antibody [EPR28774-92] (ab317573)Anti-ABCB4 antibody [EPR28774-92] (ab317573) at 1/1000 dilution + McA-RH7777 (rat liver epithelial cell) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 142 kDa
Observed band size: 140-150 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 28220208).
The identity of the bands at 37 kDa and 60 kDa are unknown.
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
-
![Western blot - Anti-ABCB4 antibody [EPR28774-92] (ab317573)](https://www.abcam.cn/ps/products/317/ab317573/Images/ab317573-542235-anti-abcb4-antibody-epr28774-92-western-blot-293t-cells-transfected-with-an-empty-vector-containing-a-his-tag-whole-cell-lysate-20-ug.jpg)
Western blot - Anti-ABCB4 antibody [EPR28774-92] (ab317573)All lanes : Anti-ABCB4 antibody [EPR28774-92] (ab317573) at 1/1000 dilution
Lane 1 : 293T cells transfected with an empty vector containing a His-tag, whole cell lysate at 20 µg
Lane 2 : 293T cells transfected with a Human ABCB4 expression vector containing a His-tag, whole cell lysate at 40 µg
Lane 3 : 293T cells transfected with a Human ABCB1 expression vector containing a His-tag, whole cell lysate at 10 µg
Lane 4 : 293T cells transfected with a Human ABCB5 expression vector containing a His-tag, whole cell lysate at 40 µg
Lane 5 : 293T cells transfected with a Human ABCB11 expression vector containing a His-tag, whole cell lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Predicted band size: 142 kDa
Observed band size: 140-250 kDa why is the actual band size different from the predicted?
Exposure time: 37 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
This antibody does not cross-react with human ABCB5, ABCB1, ABCB11.
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
In Western blot, Anti-Myc tag® antibody staining at 1/5000 dilution.
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![Anti-ABCB4 antibody [EPR28774-92] (ab317573)](https://www.abcam.cn/ps/products/317/ab317573/Images/ab317573-1-benefitsofrecombinantantibodies.jpg)
Anti-ABCB4 antibody [EPR28774-92] (ab317573)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
ab317573 尚未被引用在任何文献中。