为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 定制化产品 & 合作关系
    定制化产品 & 合作关系

    定制化产品和商务合作加速您的诊断和治疗项目

    定制化产品

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

Neuroscience Cell Type Marker Glia marker Astrocyte marker

Anti-ABCA1抗体(ab7360)

  • Datasheet
  • SDS
Reviews (6)Q&A (15)References (59)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

运输费用

Promotion Information

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Western blot - Anti-ABCA1 antibody (ab7360)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCA1 antibody (ab7360)
  • Western blot - Anti-ABCA1 antibody (ab7360)
  • Western blot - Anti-ABCA1 antibody (ab7360)

Key features and details

  • Rabbit polyclonal to ABCA1
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

你可能会感兴趣

蛋白
Recombinant Human ABCA1 protein (ab125995)
二抗
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
一抗
Product image
Anti-ABCG8 antibody (ab223056)

查看更多相关产品

概述

  • 产品名称

    Anti-ABCA1抗体
    参阅全部 ABCA1 一抗
  • 描述

    兔多克隆抗体to ABCA1
  • 宿主

    Rabbit
  • 特异性

    ab7360 has previously been shown to work well in ICC/IF on HeLa cells. However recent batches of this antibody, do not work in this application. We would recommend ab18180 for researchers wanting to detect ABCA1 in ICC/IF. For further information, please contact our Scientific Support Team.
  • 经测试应用

    适用于: WB, IHC-Pmore details
  • 种属反应性

    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Human ABCA1. Synthetic peptide: The immunogen is generated from within residues 1100-1300 of Human ABCA1
    Database link: O95477

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Neuroscience
    • Cell Type Marker
    • Glia marker
    • Astrocyte marker
    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Cholesterol Metabolism
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Cardiovascular
    • Atherosclerosis
    • Lipoprotein metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Cholesterol Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipoprotein metabolism
    • Metabolism
    • Types of disease
    • Cancer
    • Metabolism
    • Types of disease
    • Heart disease
    • Neuroscience
    • Processes

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human ABCA1 protein (ab125995)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab7360于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (5)
1/500 - 1/1000. Predicted molecular weight: 220 kDa. Additional non-specific bands are seen at lower molecular weights, but do not interfere with the ABC1 signal. It is important not to boil the sample before loading onto the gel. Boiling can cause aggregation in large proteins, resulting in the proteins inability to enter into the gel.
IHC-P
1/500.
说明
WB
1/500 - 1/1000. Predicted molecular weight: 220 kDa. Additional non-specific bands are seen at lower molecular weights, but do not interfere with the ABC1 signal. It is important not to boil the sample before loading onto the gel. Boiling can cause aggregation in large proteins, resulting in the proteins inability to enter into the gel.
IHC-P
1/500.

靶标

  • 功能

    cAMP-dependent and sulfonylurea-sensitive anion transporter. Key gatekeeper influencing intracellular cholesterol transport.
  • 组织特异性

    Widely expressed, but most abundant in macrophages.
  • 疾病相关

    Defects in ABCA1 are a cause of high density lipoprotein deficiency type 1 (HDLD1) [MIM:205400]; also known as analphalipoproteinemia or Tangier disease (TGD). HDLD1 is a recessive disorder characterized by absence of high density lipoprotein (HDL) cholesterol from plasma, accumulation of cholesteryl esters, premature coronary artery disease (CAD), hepatosplenomegaly, recurrent peripheral neuropathy and progressive muscle wasting and weakness.
    Defects in ABCA1 are a cause of high density lipoprotein deficiency type 2 (HDLD2) [MIM:604091]; also known as familial hypoalphalipoproteinemia (FHA). HDLD2 is inherited as autosomal dominant trait. It is characterized by moderately low HDL cholesterol, predilection toward premature coronary artery disease (CAD) and a reduction in cellular cholesterol efflux.
  • 序列相似性

    Belongs to the ABC transporter superfamily. ABCA family.
    Contains 2 ABC transporter domains.
  • 结构域

    Multifunctional polypeptide with two homologous halves, each containing an hydrophobic membrane-anchoring domain and an ATP binding cassette (ABC) domain.
  • 翻译后修饰

    Phosphorylation on Ser-2054 regulates phospholipid efflux.
    Palmitoylation by DHHC8 is essential for membrane localization.
  • 细胞定位

    Membrane.
  • Target information above from: UniProt accession O95477 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 19 Human
    • Entrez Gene: 11303 Mouse
    • Omim: 600046 Human
    • SwissProt: O95477 Human
    • SwissProt: P41233 Mouse
    • Unigene: 429294 Human
    • Unigene: 277376 Mouse
    • 别名

      • ABC 1 antibody
      • ABC Transporter 1 antibody
      • ABC-1 antibody
      • ABC1 antibody
      • ABCA 1 antibody
      • ABCA1 antibody
      • ABCA1_HUMAN antibody
      • ATP binding Cassette 1 antibody
      • ATP binding cassette sub family A ABC1 member 1 antibody
      • ATP binding cassette sub family A member 1 antibody
      • ATP binding Cassette Transporter 1 antibody
      • ATP-binding cassette 1 antibody
      • ATP-binding cassette sub-family A member 1 antibody
      • ATP-binding cassette transporter 1 antibody
      • CERP antibody
      • Cholesterol efflux regulatory protein antibody
      • FLJ14958 antibody
      • HDLDT1 antibody
      • Membrane bound antibody
      • MGC164864 antibody
      • MGC165011 antibody
      • TD antibody
      • TGD antibody
      see all

    图片

    • Western blot - Anti-ABCA1 antibody (ab7360)
      Western blot - Anti-ABCA1 antibody (ab7360)
      All lanes : Anti-ABCA1 antibody (ab7360)

      Lane 1 : RAW 264.7 whole cell lysate treated with T0901317
      Lane 2 : RAW 264.7 whole cell lysate untreated

      Lysates/proteins at 25 µg per lane.

      Predicted band size: 220 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCA1 antibody (ab7360)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCA1 antibody (ab7360)

      ab7360, staining ABCA1 in human prostate epithelium showing luminal and membrane staining by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

    • Western blot - Anti-ABCA1 antibody (ab7360)
      Western blot - Anti-ABCA1 antibody (ab7360)

      The experiment was performed by treating RAW macrophages with 9-cis-retinoic acid and 22R-hydroxycholesterol, known inducers of ABCA1expression in macrophages. Then total cell post-nuclear lysate (40ug protein) was separated by SDS-PAGE and detected using a 1:1000 dilution of ab7360 affinity purified Lot G incubated for 1 hour at room temperature (Lane A). Although there are lower molecular weight bands on the blot, the ABCA1 signal is excellent and gives the expected 3 bands. It is not known why ABCA1 runs as three bands, but it has been found to do so by many researchers. It is probably due to protein modifications such as glycosylation. The antibody was also tested against ABCA1 transiently expressed in 293 cells as an independent test with excellent results.

      The experiment was performed by treating RAW macrophages with 9-cis-retinoic acid and 22R-hydroxycholesterol, known inducers of ABCA1expression in macrophages. Then total cell post-nuclear lysate (40ug protein) was separ
    • Western blot - Anti-ABCA1 antibody (ab7360)
      Western blot - Anti-ABCA1 antibody (ab7360)
      Detection of ABCA1 in mouse peritoneal macrophages using ab7360. ECL exposure, 1 min.
      Lane 4: T09 uninduced lysate
      Lane 5: T09 induced lysate Detection of ABCA1 in mouse peritoneal macrophages using ab7360. ECL exposure, 1 min.
      Lane 4: T09 uninduced lysate
      Lane 5: T09 induced lysate

    实验方案

    • Recommended protocol for WB with ab7360

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (59)

    发表研究结果有使用 ab7360?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab7360 被引用在 59 文献中.

    • Lu Q  et al. Selective activation of ABCA1/ApoA1 signaling in the V1 by magnetoelectric stimulation ameliorates depression via regulation of synaptic plasticity. iScience 25:104201 (2022). PubMed: 35479414
    • Cheng WL  et al. ALK7 Acts as a Positive Regulator of Macrophage Activation through Down-Regulation of PPAR? Expression. J Atheroscler Thromb 28:375-384 (2021). PubMed: 32641645
    • Wilkinson H  et al. PAR-1 signaling on macrophages is required for effective in vivo delayed-type hypersensitivity responses. iScience 24:101981 (2021). PubMed: 33458623
    • Gong FH  et al. Attenuated macrophage activation mediated by microRNA-183 knockdown through targeting NR4A2. Exp Ther Med 21:300 (2021). PubMed: 33717243
    • Han M  et al. Therapeutic implications of altered cholesterol homeostasis mediated by loss of CYP46A1 in human glioblastoma. EMBO Mol Med 12:e10924 (2020). PubMed: 31777202
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 21 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-ABCA1 antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa and pluripotent cells)
    Permeabilization
    Yes - Triton X-100
    Specification
    HeLa and pluripotent cells
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Apr 14 2016

    Western blot abreview for Anti-ABCA1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (SCC61 cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    40 µg
    Specification
    SCC61 cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Nov 13 2015

    Western blot abreview for Anti-ABCA1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (human astrocyte)
    Loading amount
    25 µg
    Specification
    human astrocyte
    Treatment
    treated with LXR ligand for 20 hrs
    Gel Running Conditions
    Reduced Denaturing (4-15% gradient gel)
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Aug 06 2009

    Western blot abreview for Anti-ABCA1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293, THP1, mouse BMDM, mouse tissue)
    Loading amount
    15 µg
    Specification
    HEK293, THP1, mouse BMDM, mouse tissue
    Treatment
    Transfected with ABCA1 expression plasmid, Treat with 1uM TO-1317 for 24hr
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    5%milk+1%BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jan 26 2008

    Western blot abreview for Anti-ABCA1 antibody

    Poor
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (macrophage cell line)
    Loading amount
    15 µg
    Specification
    macrophage cell line
    Treatment
    0.3mM dbcAMP 24 hr
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Sep 18 2006

    Question

    Dear Abcam,

    My apologies for the late response. After some consideration we would like to know if it is possible to receive the ab7360 (rabbit anti-ABCA1 antibody) as a replacement of the ab18180 antibody, since we have obtained good results with this ab7360 antibody in the past.

    Thank you for your help,

    Read More

    Abcam community

    Verified customer

    Asked on Nov 30 2012

    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued an alternative free of charge replacement of ab7360 with the order number 1214026.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

    Abcam Scientific Support

    回复于 Nov 30 2012

    Question

    Dear Tineke/Abcam,

    We purchased the following Abcam antibodies from Sapphire Biosciences as one single order which were shipped to us as a single lot:

    1 AB68466 Rabbit polyclonal to PSAP

    1 AB36970 Rabbit polyclonal to Scavenger Receptor BI + BII

    1 AB7360 Rabbit polyclonal to ABCA1

    1 AB3366 Mouse monoclonal [JSB-1] to P Glycoprotein

    1 AB117778 Rabbit polyclonal to Cingulin

    1 AB72978 Mouse polyclonal to CNKSR3 Based on our published work these 4 proteins should be expressed in differentiated Caco-2 cells

    1 AB111692 Rabbit polyclonal to MAGI3

    1 AB83891 Rabbit polyclonal to MPP5


    The only one that has worked satisfactorily is AB68466 Rabbit polyclonal to PSAP (see attached file showing these blots). All the others have failed to work as promised. Please note, as previously mentioned, we have used a variety of exposure times with our blots – we have routinely used 10 different exposure times (as the signal increased and also when the signal would have been waning – depending on the results) for each blot using your antibodies to try and optimise what we can visualise with our blots. This has come at some considerable expense in terms of time, developing chemicals and film.

    Yes - the other antibodies we tested from our preferred supplier worked. These were purchased at the same time we purchased the Abcam antibodies but they use a different supplier in NZ so were shipped separately. They were tested at the same time we tested yours, using identical conditions and protein samples, and had very low background which is what we have come to expect from their antibodies.

    In our previous emails, we filled in questionnaires associated with the failure of AB36970 Rabbit polyclonal to Scavenger Receptor BI + BII, AB7360 Rabbit polyclonal to ABCA1, AB3366 Mouse monoclonal [JSB-1] to P Glycoprotein, and AB117778 Rabbit polyclonal to Cingulin. Despite filling in your questionnaires, you have asked for additional information, which we provided, EXCEPT for the blots as this has taken some considerable time to organise. I have been away at a conference, and as I also work part-time, this has been a pain to organise for you.

    During this time we were also evaluating the remaining 4 antibodies and have subsequently found these to be unsatisfactory too. Please see the attached questionnaires for these antibodies. This report of failure to deliver is also within the 6 month time frame of when we received your antibodies.

    Do I need to point out, yet again, that we are not stupid. You talked about the samples we tested against (rat, bovine and human Caco-2 cells) and that the antibodies we purchased were not known to cross-react with bovine samples (except MPP5 which was predicted to cross-react). The reason we chose these samples is that although our first set of experiments will ultimately be performed using differentiated Caco-2 cells, some of these targets are highly expressed in liver and we wanted to see if bovine liver could be used as a control – which is why you will note in our blots that we always ran these next to Caco-2 cell extracts to see how they compared. The same goes for the rat colon/rat colon epithelial scrapings – most of the antibodies were known to cross-react with rat or mouse as well as human proteins (also ab36970 is listed as being predicted to cross-react with rat which is not what you said in the email below). Obviously we had good reasons why we wanted to this - we will be doing animal trials in the future amongst other reasons - and again WE ALWAYS COMPARED OUR RESULTS TO OUR HUMAN CACO-2 CELL RESULTS.

    I estimate that we have probably spent more money on staff time and other lab consumables trying to get your antibodies to work and fill in your questionnaires, answer yet more questions from you, send you copies of our blots....etc....than it cost us to purchase the antibodies in the first place. This is the first time I have used Abcam antibodies as I usually purchase them from another supplier but they were unable to supply these particular antibodies. My expectation was that if your antibodies performed as expected, I would continue to purchase all my antibodies from you. Given my experience, I will not be purchasing antibodies from you in the future.

    Please either replace these antibodies immediately, or better still, please refund our money in full so we can purchase them elsewhere. I don’t want to be bothered with any further questions. You have wasted enough of our time and it’s about time you did the right thing by us.

    Read More

    Abcam community

    Verified customer

    Asked on Sep 27 2012

    Answer

    Thank you for your reply and all the additional information. This makes the problems you have been experiencing much clearer to understand. It is clear that you have spent a lot of time in trying to optimise these antibodies and I am sorry that they are not providing the expected results.

    I am sorry if you feel the questions I have been asking have not been appropriate. The reason I askedfor further information is to try to understand what has been tried already and to try to understand what may be contributing to these problems. With a total of 7 antibodies not working as expected we are taking this very seriously to try and isolate the reason and to offer you a suitable solution as well as preventing this from happening again in the future.

    I have been discussing this case with my colleague Dr. Bagrij who has experience in working with a number of the targets you are interested in. I understand the Caco2 lysate you have is very limited and I don't want you to use any more in optimising any of these antibodies. I think it would be worthwhile to try a different lysate to the ones you have been using to see if this makes a difference in the results observed. To this end I would like to send you a pre-prepared Jurkat lysate which have been used as positive controls for the anti-Cingulin antibody (ab117778) and the anti-MPP5 antibody (ab83891). Would you mind trying this lysate with these two antibodies just to see if the expected signal is observed?

    You mentioned that you have previously published in regards to the Caco2 cells expressing a number of these targets. Would you mind sharing this reference with me? This information would be very useful in this investigation as well asfor other customers using these antibodies.

    Could you also confirm on which date you received the 8 antibodies? I know which order they came from but I want to make sure they reached youwithin the expected time.Were they all delivered in the same package? What was the package like?

    I am sorry that you are having these problemsand Iwould like to assure you that I amtrying my best to get to the bottom of this and provide you with a solution.

    I look forward to receiving your reply.

    Read More

    Abcam Scientific Support

    回复于 Sep 27 2012

    Question

    Please see below for the response from the customer;

    I’m very frustrated by Abcam’s response. It took a long time to fill in the questionnaires for each of the antibodies and some of their additional questions were already answered in the questionnaire (Q3 and Q6). Q6 is quite insulting, even more so when I had specifically answered that I’d used anti-mouse secondary antibodies.

    It also took them 4-5 working days to reply to the questionnaire answers, now they want more information, taking more of my time, and I guess I have to wait another 4-5 working days for their next response.

    Quite frankly, this is unacceptable.

    In short,

    Q1. the same blocking agent was used consistently throughout the western experiment. So, if BSA was used for blocking, it was also used for the primary and the secondary. When it didn’t work, we tried milk etc.

    Q2. For most of the repeats, blots weren’t stripped. We made ran fresh gels and blotted these (exactly the same loading concentrations and conditions). Therefore, stripping was not the issue. ALWAYS, we ran another gel with the same loading concentration which was stained with coomassie. ALWAYS we ran coloured markers so we could check that the protein had transferred. We have never had trouble with transfer using the i-blot.

    Q3. Please read the answers I have already supplied.

    Q4. Exposure times varied from 15 s through to 15 min. We also used 2 pieces of film so that the bottom film acted as a filter. We developed at least 8 pieces of film for each blot each time we repeated it and also used a range of exposure times. Faint blots were left longer while those with high background were just left for a few seconds. High background blots were also re-exposed after 1-2 hours as the signal was waning. We covered the entire range.

    Q5. This will take a lot of time as we’ve done lots of blots. They will have to insist this is absolutely necessary then pay for my time. It’s their antibodies that aren’t working so I shouldn’t have to defend my results. My time will probably cost more than their antibodies do to replace.

    Q6. The secondary antibodies were brand new and worked because, as previously answered, we had magic markers which only show up if the secondary antibody is working.

    My expectation is that Abcam will hurry up and come to the party with new, replacement antibodies in a timely manner and stop wasting our time. If they don’t, we won’t be using them again and as we work for a large research institution with a large international network of collaborators, won’t be recommending them to anyone.

    Read More

    Abcam community

    Verified customer

    Asked on Aug 28 2012

    Answer

    Thank you for your reply.

    I am very sorry that you have not been satisfied with the way we have handeled your complaints. I do understand and appreciate the amount of time you have spent it the laboratory. Our policy is that we are happy to offer a refund, credit note or free of charge replacement when a product is not working in a successfully tested applications or species (and the product has been purchased in the last 180 days).

    However, we often find that suggesting some scientifically thought out optimisation tips improves the results. This is obviously a preferable outcome.So I hope you can understand that our policy is to offer the best technical support possible to help optimise the results first.

    It is also important for us to understand what is contributing to the problems encountered, as if there is a problem with the antibody, forexample with a particular lot, we need to try to find out why this has happened and if any other customers may be affected and if we need to let them know.

    I am sure you can understand that with four antibodies not providing satisfactory results for you we need to take it especially seriously, I do not want to simply offer replacement for them to perform in exactly the same way and further waste your time.

    I am sorry that I did not read the information regarding the anti-mouse antibody used with ab3366 properly. This was an error on my part and I apologize. The reason I wanted to have a look at the images of the blots was to try to ascertain where the high background may be coming from. With the antibodies used, none have been tested with bovine samples so we would not be able to know if they would cross react or not. Similarly, ab7360 and ab36970 have not been tested with rat samples as yet, although they would be predicted to cross-react due to their sequence homology. That leaves onlytheCaco-2 cell samples for ab7360 and ab36970. It would be very helpful to understand the results observed more fully if you would be able to provide a few representative images of the blots obtained.

    You have mentioned that other antibodies have been used with the samples and have performed as expected. Are any of these from Abcam, or are they from other companies? Were any of them delivered in the same shipment as the 4 antibodies which are not working? I am trying to ascertain if there may have been a problem with your particular delivery which affected the antibody activity. This is important as with some of the antibodies involved, we only have the same lots available as you have already tried and I need to ascertain if this is worth trying again for you.

    I am sorry for the delay youhave experienced in our handling of your complaints. I am sorry for this and the inconvenience caused. I will try to resolve the situation as quickly as I can.

    Read More

    Abcam Scientific Support

    回复于 Aug 28 2012

    Question

    Hi Abcam,

    Please find attached 4 technical questionnaires from the same customer, I thought it would be easier if I attached them all in this email rather than filling out a separate technical enquiry for each one. If another method is preferred please let me know.

    AB36970, AB7360, AB336 & AB68466

    Abcam Invoice #: xxxxxx
    Thanks so much for your help, from what I understand the customer would like a replacement of each antibody to be sent if they are eligible.

    Kind regards,

    Read More

    Abcam community

    Verified customer

    Asked on Aug 23 2012

    Answer

    Thank you for reporting the problems encountered with these antibodies and for taking the time to complete the questionnaire. I am sorry to hear that these antibodieshave not providing satisfactory results so far.

    The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality.

    Having reviewed this case, there are a few further details I would like clarified if possible:

    1. You have mentioned that BSA, milk and Roti block were tried. When each blocking agent was tried, was the same blocking used in the primary antibody diluent? For example, when using the BSA blokcing, did you use BSA in the antibody diluent or did you continue with milk for this? Was this the case for the secondary antibody as well?

    2. Could you explain how the membranes were stripped between each staining?

    3. Were the same transfer conditions used for each antibody?

    4. What exposure times were used?

    5. Could youshare some images of the blots obtained? With each lane labelled with the samples and the molecular weight markers. If its not too much trouble, could the images of the blotting with the xxxxxx antibody and the acting loading control also be included. This would help greatly in understanding more fully the problems encountered with the antibodies.

    6. With the anti-P Glycoprotein antibody (ab3366), did you use the same secondary antibody as with the other antibodies? Please bear in mind that this is a mouse monoclonal antibody and will not be detected by the anti-rabbit secondary antibody. Was a different antibody used? If so, has this one been used successfully previously?

    In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund.

    I look forward to receiving your reply.

    Read More

    Abcam Scientific Support

    回复于 Aug 23 2012

    Question

    DESCRIPTION OF THE PROBLEM No signal or weak signal SAMPLE human liver membrane, liver microsome and liver postnuclear lysates PRIMARY ANTIBODY 1:1500 in 3% milk TBST DETECTION METHOD ECL POSITIVE AND NEGATIVE CONTROLS USED no ANTIBODY STORAGE CONDITIONS 4 degree SAMPLE PREPARATION sucrose, tris, et. beta-meceptoethonal at room temperature 20minutes or DTT 37 degree 30 minutes AMOUNT OF PROTEIN LOADED 40microgram ELECTROPHORESIS/GEL CONDITIONS 3-6% SDS PAGE or 3-8%SDS-PAGE TRANSFER AND BLOCKING CONDITIONS Tris-Glycine buffer with or without SDS, 10 or 20% methonal Blocking: 5% milk TBST SECONDARY ANTIBODY Donkey anti rabbit F(ab)'2 fragment HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 10 HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes ADDITIONAL NOTES Others I follow the protocol on Another companies datasheet for ABCA1 antibody.

    Read More

    Abcam community

    Verified customer

    Asked on Sep 18 2006

    Answer

    I'm sorry to hear you are having a problem with ab7360. I would like to suggest the following modifications to your protocol: The recommended dilution range for this antibody is 1/500 - 1/1000. You may be using it at too high a dilution. I did not see your incubation conditions with this antibody from your enquiry. Since you are seeing no signal, I would suggest removing the milk from the primary antibody solution and incubating at 4C overnight. The milk in the primary antibody solution can overblock the membrane, preventing the antibody from binding to its target. I would continue incubating the sample in loading buffer for 30 minutes at 37C. This protein can form aggregates, preventing migration into the gel matrix. For how long did you block? If you have blocked for longer than one hour, you may wish to reduce this step to 1 hour, or even 30 minutes. Did you check (e.g. Ponceau stain) to see that the transfer was efficient? Please let me know if this helps and do not hesitate to contact us for further advice.

    Read More

    Abcam Scientific Support

    回复于 Sep 18 2006

    1-10 of 21 Abreviews or Q&A

    •  向后
    • 1
    • 2
    • 3
    • 向前 

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    通过邮件收到学术资源和促销信息 注册
    研究类别(A-Z)
    • 肿瘤研究
    • 心血管研究
    • 细胞生物学
    • 发育生物学
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 代谢
    • 微生物学
    • 神经生物学
    • 信号转导研究
    • 干细胞研究
    产品种类(A-Z)
    • 一抗
    • 二抗
    • 生化试剂
    • 亚类对照
    • 流式细胞仪多色选择器
    • 试剂盒
    • 内参对照
    • 细胞裂解液
    • 多肽
    • 蛋白酶
    • 组织切片
    • 蛋白标签及细胞标记物
    • 相关试剂及工具
    帮助与支持
    • 技术支持
    • 技术FAQ
    • 实验步骤与建议
    • 购买FAQ
    • RabMAb 产品
    • 培训课程
    • 按靶标浏览
    企业
    • 企业资讯
    • 投资者关系
    • 公司新闻
    • 招贤纳士
    • 关于我们
    活动通知
    • 商品展览
    • 会议
    全球网站
    • abcam.com
    • abcam.co.jp
    QR code for Weechat

    微信扫码 关注我们

    沪ICP备14046373号-1

    沪公网安备 31011502007413号

    • 销售条款
    • 网站使用条款
    • Cookie政策
    • 隐私权政策
    • 法律
    © 1998-2023 艾博抗(上海)贸易有限公司版权所有