Name: Anti-ABAT/GABA-T antibody [EPR4433]
Brand: RabMAb
SKU: ab108249
Rating: 5 (2 reviews)

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Western blot - Anti-ABAT/GABA-T antibody [EPR4433] (ab108249)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4433] to ABAT/GABA-T
Suitable for: WB, IP, IHC-P
Reacts with: Mouse, Rat, Human

Carrier Free

产品名称

Anti-ABAT/GABA-T抗体[EPR4433]
参阅全部 ABAT/GABA-T 一抗
描述

兔单克隆抗体[EPR4433] to ABAT/GABA-T
宿主

Rabbit
特异性

Mouse cross reactivity tested by western blot and IHC. Rat cross reactivity tested by western blot only.
经测试应用

适用于: WB, IP, IHC-Pmore details
不适用于: Flow Cyt or ICC/IF
种属反应性

与反应: Mouse, Rat, Human
免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
阳性对照
- HepG2 cell lysate, SH-SY5Y cell lysae, Human fetal liver tissue lysate, rat brain tissue lysate, rat kidney tissue lysate, mouse brain tissue lysate, Human liver tissue.
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
纯度

Protein A purified
克隆

单克隆
克隆编号

EPR4433
同种型

IgG
研究领域

Alternative Versions
- Anti-ABAT/GABA-T antibody [EPR4433] - BSA and Azide free (ab232525)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- Mouse brain tissue lysate - total protein (ab30151)
Recombinant Protein
- Recombinant Human ABAT/GABA-T protein (ab152164)

The Abpromise guarantee

Abpromise™承诺保证使用ab108249于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
WB	(2)	1/10000 - 1/50000. Predicted molecular weight: 56 kDa.
IP		1/10 - 1/100.
IHC-P		1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. Antigen retrieval is recommended For unpurified use at 1/250 - 1/500 dilution.

说明
WB 1/10000 - 1/50000. Predicted molecular weight: 56 kDa.
IP 1/10 - 1/100.
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. Antigen retrieval is recommended For unpurified use at 1/250 - 1/500 dilution.

应用说明

Is unsuitable for Flow Cyt or ICC/IF.

功能

Catalyzes the conversion of gamma-aminobutyrate and L-beta-aminoisobutyrate to succinate semialdehyde and methylmalonate semialdehyde, respectively. Can also convert delta-aminovalerate and beta-alanine.
组织特异性

Liver > pancreas > brain > kidney > heart > placenta.
疾病相关

Defects in ABAT are a cause of gamma-aminobutyrate transaminase deficiency (GABA-AT deficiency) [MIM:613163]. The phenotype of this deficiency includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities.
序列相似性

Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.
细胞定位

Mitochondrion matrix.
Target information above from: UniProt accession P80404 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 18 Human
- Entrez Gene: 268860 Mouse
- Entrez Gene: 81632 Rat
- Omim: 137150 Human
- SwissProt: P80404 Human
- SwissProt: P61922 Mouse
- SwissProt: P50554 Rat
- Unigene: 336768 Human
- Unigene: 259315 Mouse
see all
别名
- (S) 3 amino 2 methylpropionate transaminase antibody
- (S)-3-amino-2-methylpropionate transaminase antibody
- 4 aminobutyrate aminotransferase antibody
- 4 aminobutyrate aminotransferase, mitochondrial antibody
- 4-aminobutyrate aminotransferase antibody
- ABAT antibody
- FLJ17813 antibody
- FLJ30272 antibody
- GABA aminotransferase antibody
- GABA AT antibody
- GABA T antibody
- GABA transaminase antibody
- GABA transferase antibody
- GABA-AT antibody
- GABA-T antibody
- GABAT antibody
- GABT_HUMAN antibody
- Gamma amino N butyrate transaminase antibody
- Gamma-amino-N-butyrate transaminase antibody
- hCG1984265 antibody
- L AIBAT antibody
- L-AIBAT antibody
- LAIBAT antibody
- mitochondrial antibody
- NPD009 antibody
see all

Western blot - Anti-ABAT/GABA-T antibody [EPR4433] (ab108249)

All lanes : Anti-ABAT/GABA-T antibody [EPR4433] (ab108249) at 1/10000 dilution (Purified)

Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates with 5% NFDM /TBST
Lane 2 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates with 5% NFDM /TBST
Lane 3 : Mouse brain lysates with 5% NFDM /TBST
Lane 4 : Rat brain lysates with 5% NFDM /TBST

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 56 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABAT/GABA-T antibody [EPR4433] (ab108249)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat liver tissue sections labeling ABAT/GABA-T with Purified ab108249 at 1:1000 dilution (0.9 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)
Immunoprecipitation - Anti-ABAT/GABA-T antibody [EPR4433] (ab108249)

ab108249 (purified) at 1:40 dilution (2µg) immunoprecipitating ABAT/GABA-T in HepG2 whole cell lysate.
Lane 1 (input): HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab108249 & HepG2 whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab108249 in HepG2 whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABAT/GABA-T antibody [EPR4433] (ab108249)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cerebrum tissue sections labeling ABAT/GABA-T with Purified ab108249 at 1:1000 dilution (0.9 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABAT/GABA-T antibody [EPR4433] (ab108249)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling ABAT/GABA-T with Purified ab108249 at 1:1000 dilution (0.9 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABAT/GABA-T antibody [EPR4433] (ab108249)

Unpurified ab108249, at 1/250 dilution, staining ABAT/GABA-T in Human liver tissue by immunohistochemistry.
Western blot - Anti-ABAT/GABA-T antibody [EPR4433] (ab108249)

All lanes : Anti-ABAT/GABA-T antibody [EPR4433] (ab108249) (unpurified)

Lane 1 : HepG2 cell lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : Human fetal liver tissue lysate
Lane 4 : rat brain tissue lysate
Lane 5 : rat kidney tissue lysate
Lane 6 : mouse brain tissue lysate

Predicted band size: 56 kDa
Anti-ABAT/GABA-T antibody [EPR4433] (ab108249)

Flow cytometry protocols
Immunoprecipitation protocols
Immunohistochemistry protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

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发表研究结果有使用 ab108249？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab108249 被引用在 8 文献中.

Zhang M et al. Gamma-Aminobutyrate Transaminase Protects against Lipid Overload-Triggered Cardiac Injury in Mice. Int J Mol Sci 23:N/A (2022). PubMed: 35216295
Joseph LC et al. Combined metabolomic and transcriptomic profiling approaches reveal the cardiac response to high-fat diet. iScience 25:104184 (2022). PubMed: 35494220
Park MG et al. High-yield synthesis and purification of recombinant human GABA transaminase for high-throughput screening assays. J Enzyme Inhib Med Chem 36:2016-2024 (2021). PubMed: 34514924
Si W et al. Effect of GABA-T on Reproductive Function in Female Rats. Animals (Basel) 10:N/A (2020). PubMed: 32230949
Puleston DJ et al. Polyamines and eIF5A Hypusination Modulate Mitochondrial Respiration and Macrophage Activation. Cell Metab 30:352-363.e8 (2019). PubMed: 31130465
Chen L et al. Involvement of upregulation of miR-210 in a rat epilepsy model. Neuropsychiatr Dis Treat 12:1731-7 (2016). WB . PubMed: 27471387
De Ita-Pérez D et al. Synchronization by food access modifies the daily variations in expression and activity of liver GABA transaminase. Biomed Res Int 2014:590581 (2014). WB ; Rat . PubMed: 24809054
Jo S et al. GABA from reactive astrocytes impairs memory in mouse models of Alzheimer's disease. Nat Med N/A:N/A (2014). Mouse . PubMed: 24973918

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Application

Western blot

Sample

Sheep Tissue lysate - whole (Liver tissue)

Gel Running Conditions

Reduced Denaturing

Loading amount

20 µg

Specification

Liver tissue

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Feb 14 2018

Application

Western blot

Sample

Human Cell lysate - whole cell (breast cancer)

Gel Running Conditions

Reduced Denaturing

Loading amount

20 µg

Specification

breast cancer

Blocking step

Milk as blocking agent for 1 hour(s) and 23 minute(s) · Concentration: 0.5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Dec 05 2016

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Key features and details

Related conjugates and formulations

概述

产品名称

描述

宿主

特异性

经测试应用

种属反应性

免疫原

阳性对照

常规说明

性能

形式

存放说明

存储溶液

纯度

克隆

克隆编号

同种型

研究领域

相关产品

Alternative Versions

Compatible Secondaries

Isotype control

Positive Controls

Recombinant Protein

应用

The Abpromise guarantee

靶标

功能

组织特异性

疾病相关

序列相似性

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

文献 (8)

客户评价及客户问答

Western blot abreview for Anti-ABAT/GABA-T antibody [EPR4433]

Western blot abreview for Anti-ABAT/GABA-T antibody [EPR4433]