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AB315826

重组Anti-AAV9抗体[HL2374-IgG1] - BSA and Azide free

Anti-AAV9 antibody [HL2374-IgG1] - BSA and Azide free

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Mouse Recombinant Monoclonal AAV9 antibody. Carrier free. Suitable for I-ELISA, Dot and reacts with Recombinant fragment - Adeno-associated virus 9 samples.

查看别名

Adeno-associated virus - 9

2 Images
Indirect ELISA - Anti-AAV9 antibody [HL2374-IgG1] - BSA and Azide free (AB315826)
  • I-ELISA

Lab

Indirect ELISA - Anti-AAV9 antibody [HL2374-IgG1] - BSA and Azide free (AB315826)

Indirect ELISA showing ab315826 binding to AAV9 empty capsid. Binding of ab315826 was assessed in a serial dilution and EC-50 is 8.62 ng/ml. The secondary antibody used was Goat Anti-Mouse IgG H&L (HRP) preadsorbed at 1/2000 dilution.

Antigen : AAV1 empty capsid,AAV2 empty capsid,AAV3B empty capsid,AAV5 empty capsid,AAV6 empty capsid,AAV8 empty capsid,AAV9 empty capsid.

Antigen concentration : 7.5E+10 vp/mL

Dot Blot - Anti-AAV9 antibody [HL2374-IgG1] - BSA and Azide free (AB315826)
  • Dot

Supplier Data

Dot Blot - Anti-AAV9 antibody [HL2374-IgG1] - BSA and Azide free (AB315826)

Dot blot analysis of AAV9 using ab315826 at 1 : 2000 (0.5 ug/ml) followed by a Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1 : 5000 dilution.

Dot blot was performed using 1 ng of each capsid loaded per well.

Anti-AAV9 antibody [HL2368] (ab315818) (1 : 2000), Anti-AAV9 antibody [HL2370] (ab315820) (1 : 2000), Anti-AAV9 antibody [HL2374] (ab315823) (1 : 2000), and Anti-AAV9 antibody [HL2374-IgG1] - BSA and Azide free (ab315826) (1 : 2000) specifically recognize AAV9 capsid.

Anti-AAV8 + AAV3B antibody [HL2383] (ab315827) (1 : 2000), and Anti-AAV8 + AAV3B antibody [HL2383-IgG1] (ab315829) (1 : 2000) specifically recognize AAV8/3B capsid.

Anti-AAV8 + AAV9 antibody [HL2372] (ab315821) (1 : 2000) specifically recognizes AAV8/9 capsid.

Anti-AAV5 antibody [HL2476] (ab315831) (1 : 2000) specifically recognizes AAV5 capsid.

false

Exposure time: 8min

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

HL2374-IgG1

亚型

IgG1

不含载体蛋白

Yes

反应种属

Adeno-associated virus 9

应用

Dot, I-ELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Adeno-associated virus 9": { "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "" }, "Recombinant fragment - Adeno-associated virus 9": { "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "1000 ng/mL", "IELISA-species-notes": "<p></p>", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/2000", "Dot-species-notes": "<p></p>" } } }
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产品详情

ab315826 is the carrier-free version of ab315825.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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性能和储存信息

形式
Liquid
纯化工艺
Ion exchange chromatography
存储溶液
pH: 7.2 - 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Adeno-associated virus serotype 9 (AAV9) is a viral vector used in gene therapy known for its ability to transduce cells by delivering genetic material. Popular as a genetic tool AAV9 is utilized because of its efficient transfection capabilities in a variety of tissues. It consists of a protein capsid which forms an icosahedral structure important for its ability to encapsulate and deliver therapeutic DNA. The AAV9 capsid has an approximate molecular mass of 60 kilodaltons per subunit assembling into a larger capsid particle. AAV9 exhibits a broad expression profile being able to transduce diverse tissues including muscle liver central nervous system and cardiac tissues.
Biological function summary

AAV9 targets enable the efficient delivery of genes by trafficking across the cellular barriers leading to gene expression within target cells. Part of the larger family of adeno-associated viruses AAV9's specificity for different tissues allows researchers to target certain organs for therapeutic purposes. The AAV9 capsid engages with cell surface receptors and undergoes internalization by endocytosis. Interestingly different serotypes within this family vary slightly in tissue tropism and selection of the appropriate serotype affects therapeutic outcomes.

Pathways

AAV9 interacts with cellular regulatory mechanisms involved in transporting genetic material to the nucleus where it integrates into the genome or exists episomally. It is notably applied in pathways related to muscle and CNS diseases where AAV9 enhances the delivery of therapeutic genes. It operates in conjunction with certain cellular proteins that facilitate endosomal escape and nuclear entry making it effective for addressing genetic defects linked with these pathways.

AAV9 has been linked to therapeutic strategies for conditions such as spinal muscular atrophy (SMA) and certain types of muscular dystrophy. Utilization of AAV9 allows for the delivery of corrective genes potentially reversing or halting disease progression. In SMA for example AAV9 is used to deliver survival motor neuron (SMN) gene which is deficient in patients. Similarly in muscular dystrophy AAV9 aids in the administration of dystrophin or microdystrophin genes which play an important role in muscle integrity and function.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

See full target information AAV9
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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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