AB315831

重组Anti-AAV5抗体[HL2476]

Anti-AAV5 antibody [HL2476]

Recombinant
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Mouse Recombinant Monoclonal AAV5 antibody. Suitable for I-ELISA, Dot and reacts with Recombinant fragment - adeno-associated virus 5 samples.

2 Images

Indirect ELISA - Anti-AAV5 antibody [HL2476] (AB315831)

I-ELISA

Lab

Indirect ELISA - Anti-AAV5 antibody [HL2476] (AB315831)

Indirect ELISA showing ab315831 binding to AAV5 empty capsid. Binding of ab315831 was assessed in a serial dilution and EC-50 is 1.974 ng/ml. The secondary antibody used was Goat Anti-Mouse IgG H&L (HRP) preadsorbed at 1/2000 dilution.

Antigen : AAV1 empty capsid,AAV2 empty capsid,AAV5 empty capsid,AAV6 empty capsid,AAV8 empty capsid,AAV9 empty capsid AAV3B empty capsid.

Antigen concentration : 7.5E+10 vp/mL

Supplier Data

Dot Blot - Anti-AAV5 antibody [HL2476] (AB315831)

Dot blot analysis of AAV5 using ab315831 at 1 : 2000 (0.5 ug/ml) followed by a Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1 : 5000 dilution.

Dot blot was performed using 1 ng of each capsid loaded per well.

Anti-AAV9 antibody [HL2368] (ab315818) (1 : 2000), Anti-AAV9 antibody [HL2370] (ab315820) (1 : 2000), Anti-AAV9 antibody [HL2374] (ab315823) (1 : 2000), and Anti-AAV9 antibody [HL2374-IgG1] - BSA and Azide free (ab315826) (1 : 2000) specifically recognize AAV9 capsid.

Anti-AAV8 + AAV3B antibody [HL2383] (ab315827) (1 : 2000), and Anti-AAV8 + AAV3B antibody [HL2383-IgG1] (ab315829) (1 : 2000) specifically recognize AAV8/3B capsid.

Anti-AAV8 + AAV9 antibody [HL2372] (ab315821) (1 : 2000) specifically recognizes AAV8/9 capsid.

Anti-AAV5 antibody [HL2476] (ab315831) (1 : 2000) specifically recognizes AAV5 capsid.

false

Exposure time: 8min

不同偶联物与剂型 (1)

Carrier free

Anti-AAV5 antibody [HL2476] - BSA and Azide free

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

HL2476

亚型

IgG2b

不含载体蛋白

反应种属

adeno-associated virus 5

应用

Dot, I-ELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant fragment - adeno-associated virus 5": { "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "1000 ng/mL", "IELISA-species-notes": "<p></p>", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/2000", "Dot-species-notes": "<p></p>" }, "adeno-associated virus 5": { "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "" } } }

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产品详情

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The biological target AAV5 or Adeno-Associated Virus type 5 is a non-pathogenic virus used widely as a vector in gene therapy. This virus has a protein structure named the AAV5 capsid which serves as its protective shell. The AAV5 capsid has an estimated mass of 60 megadaltons and plays an important role in virus stability and infectivity. AAV5 is expressed in various tissues but its expression pattern can differ based on the type of tissue and species being studied.

Biological function summary

The AAV5 capsid facilitates entry into host cells by interacting with cellular receptors. It is not a component of a larger complex but its role is important for delivering genetic material into cells. The virus's ability to transduce a wide range of cell types makes it highly effective in gene therapy applications. This versatility allows researchers to target specific tissues and diseases.

Pathways

AAV5 plays an important role in gene delivery systems which can impact the expression of genetically targeted therapies. Its interaction with heparan sulfate proteoglycans during cellular entry highlights its involvement in pathways related to cellular uptake and transfer of genetic material. This function is highly relevant when considering AAV5 as a tool for introducing therapeutic genes into cells alongside proteins like other adeno-associated viruses which may share similar pathways.

AAV5 has emerged as a powerful tool in the treatment of genetic disorders such as hemophilia and neurodegenerative diseases. Its ability to transduce non-dividing cells makes it suitable for long-term expression in cells. AAV5's interaction with the coagulation factor IX protein can provide therapeutic benefits in hemophilia B patients when delivered effectively. It also shows promise in delivering genes to neural tissues potentially aiding in treating disorders like Parkinson’s disease.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

See full target information AAV5

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-AAV5抗体[HL2476]