Anti-AANAT (phospho T29)抗体(ab3439)
Key features and details
- Rabbit polyclonal to AANAT (phospho T29)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-AANAT (phospho T29)抗体
参阅全部 AANAT 一抗 -
描述
兔多克隆抗体to AANAT (phospho T29) -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Chicken, Hamster, Chimpanzee, Rhesus monkey
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免疫原
Synthetic peptide corresponding to Mouse AANAT aa 24-33 (N terminal) (phospho T29).
Sequence: CQRRHTpLPAS
(Peptide available asab4975) -
阳性对照
- Rat pineal gland whole protein extract.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Total anti-N-terminal AA-NAT-specific IgG was affinity purified on resin-coupled non-phosphopeptide. Phospho-specific N-terminal IgG was subsequently isolated on resin-coupled phosphopeptide. -
Primary antibody说明
Pineal gland Serotonin N-acetyltransferase (Arylalkylamine N-acetyltransferase, AA-NAT) is an ~23 kDa protein which is the rate-limiting enzyme in melatonin synthesis. Melatonin serves as the hormonal signal of the daily light / dark cycle, or body clock. The nocturnal oscillation in enzyme activity ranges by 10- to 100-fold depending on species among vertebrates. Studies have shown that AA-NAT is regulated by cAMP at both the mRNA and protein levels. AA-NAT has putative protein kinase A (PKA) sites at Thr 29 and Ser 206. Phosphorylation of AA-NAT at these residues might play a role in protein inactivation via proteasome-dependent degradation. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.5 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
By Western blot, this antibody detects an ~23 kDa protein representing phospho AA-NAT in rat pineal gland whole protein extract. |
| 说明 |
|---|
|
WB
Use a concentration of 0.5 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa). By Western blot, this antibody detects an ~23 kDa protein representing phospho AA-NAT in rat pineal gland whole protein extract. |
靶标
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功能
Controls the night/day rhythm of melatonin production in the pineal gland. Catalyzes the N-acetylation of serotonin into N-acetylserotonin, the penultimate step in the synthesis of melatonin. -
组织特异性
Highly expressed in pineal gland and at lower levels in the retina. Weak expression in several brain regions and in the pituitary gland. -
通路
Aromatic compound metabolism; melatonin biosynthesis; melatonin from serotonin: step 1/2. -
疾病相关
Delayed sleep phase syndrome -
序列相似性
Belongs to the acetyltransferase family. AANAT subfamily.
Contains 1 N-acetyltransferase domain. -
翻译后修饰
cAMP-dependent phosphorylation on both N-terminal Thr-31 and C-terminal Ser-205 regulates AANAT activity by promoting interaction with 14-3-3 proteins. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 503504 Chimpanzee
- Entrez Gene: 15 Human
- Entrez Gene: 11298 Mouse
- Omim: 600950 Human
- SwissProt: P79774 Chicken
- SwissProt: Q5IS55 Chimpanzee
- SwissProt: Q16613 Human
- SwissProt: O88816 Mouse
see all -
别名
- AA-NAT antibody
- Aanat antibody
- Aralkylamine N-acetyltransferase antibody
see all
图片
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Western blot - Anti-AANAT (phospho T29) antibody (ab3439)
Western blot detection of Serotonin N-acetyltransferase (phospho T29) in transfected 293T cell lysate using ab3439.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab3439 被引用在 3 文献中.
- Kim J et al. Biosynthesis of neuroprotective melatonin is dysregulated in Huntington's disease. J Pineal Res 75:e12909 (2023). PubMed: 37721126
- Hou B et al. Homeostatic Plasticity Mediated by Rod-Cone Gap Junction Coupling in Retinal Degenerative Dystrophic RCS Rats. Front Cell Neurosci 11:98 (2017). IHC (PFA fixed) ; Rat . PubMed: 28473754
- Moran AW et al. Expression of Na+/glucose co-transporter 1 (SGLT1) is enhanced by supplementation of the diet of weaning piglets with artificial sweeteners. Br J Nutr 104:637-46 (2010). PubMed: 20338074