JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB3439

Anti-AANAT (phospho T29)抗体

Anti-AANAT (phospho T29) antibody

Be the first to review this product! Submit a review

|

(3 Publications)

Rabbit Polyclonal AANAT phospho T29 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Mouse Aanat phospho T29 aa 1-50.

查看别名

Snat, Serotonin N-acetyltransferase, Serotonin acetylase, Aralkylamine N-acetyltransferase, AA-NAT

1 Images
Western blot - Anti-AANAT (phospho T29) antibody (AB3439)
  • WB

Unknown

Western blot - Anti-AANAT (phospho T29) antibody (AB3439)

Western blot detection of Serotonin N-acetyltransferase (phospho T29) in transfected 293T cell lysate using ab3439.

All lanes:

Western blot - Anti-AANAT (phospho T29) antibody (ab3439)

Predicted band size: 23 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

WB

applications

免疫原

Synthetic Peptide within Mouse Aanat phospho T29 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

O88816

style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5 µg/mL", "WB-species-notes": "<p>By Western blot, this antibody detects an ~23 kDa protein representing phospho AA-NAT in rat pineal gland whole protein extract.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
style
left-column
style
left-column
style
left-column

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
纯化说明
Total anti-N-terminal AA-NAT-specific IgG was affinity purified on resin-coupled non-phosphopeptide.Phospho-specific N-terminal IgG was subsequently isolated on resin-coupled phosphopeptide.
存储溶液
Preservative: 0.05% Sodium azide Constituents: 99% PBS, 0.1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
style
left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

AANAT also known as aralkylamine N-acetyltransferase or serotonin N-acetyltransferase is an enzyme with a molecular mass of approximately 23.3 kDa. This enzyme catalyzes the transfer of an acetyl group from acetyl-CoA to serotonin forming N-acetylserotonin. AANAT expression is mainly found in the pineal gland where it acts as an important player in melatonin biosynthesis. The function of AANAT as a regulatory point in this biosynthesis contributes to the role of melatonin in circadian rhythms and sleep regulation.
Biological function summary

AANAT has a critical role in regulating physiological processes through the production of melatonin. It operates as part of the intramolecular complex that facilitates its enzymatic activity in the pineal gland. By converting serotonin into N-acetylserotonin which further converts to melatonin AANAT modulates the synthesis of melatonin an indoleamine with effects on sleep-wake cycles and seasonal reproductive rhythms in mammals. This regulatory activity reflects how AANAT influences the hormonal signaling pathways in response to light and dark cycles.

Pathways

AANAT is integral to the melatonin synthesis pathway and interacts closely in serotonin metabolism. It tightly regulates the conversion of serotonin to melatonin in the pineal gland especially during nocturnal periods. One key protein related to AANAT in this pathway is hydroxyindole O-methyltransferase (HIOMT) which converts N-acetylserotonin to melatonin. The modulation of melatonin levels impacts diverse biological pathways including circadian rhythm regulation and neuroendocrine signaling.

AANAT plays a significant role in conditions like seasonal affective disorder (SAD) and sleep disturbances. Alterations in AANAT expression or function can affect melatonin levels leading to these disorders. Additionally disruptions in the enzymatic activity of AANAT may have interactions with serotonin influencing its availability and potentially linking it to disorders like depression. Understanding AANAT's function allows researchers to explore therapeutic strategies targeting melatonin pathways in managing these conditions.
style
left-column
style
left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

靶点信息

Controls the night/day rhythm of melatonin production in the pineal gland. Catalyzes the N-acetylation of serotonin into N-acetylserotonin, the penultimate step in the synthesis of melatonin.
See full target information Aanat phospho T29
style
left-column

文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of pineal research 75:e12909 PubMed37721126

2023

Biosynthesis of neuroprotective melatonin is dysregulated in Huntington's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Jinho Kim,Wei Li,Jingjing Wang,Sergei V Baranov,Brianna E Heath,Jiaoying Jia,Yalikun Suofu,Oxana V Baranova,Xiaomin Wang,Timothy M Larkin,William R Lariviere,Diane L Carlisle,Robert M Friedlander

Frontiers in cellular neuroscience 11:98 PubMed28473754

2017

Homeostatic Plasticity Mediated by Rod-Cone Gap Junction Coupling in Retinal Degenerative Dystrophic RCS Rats.

Applications

IHC (PFA fixed)

Species

Rat

Baoke Hou,Yan Fu,Chuanhuang Weng,Weiping Liu,Congjian Zhao,Zheng Qin Yin

The British journal of nutrition 104:637-46 PubMed20338074

2010

Expression of Na+/glucose co-transporter 1 (SGLT1) is enhanced by supplementation of the diet of weaning piglets with artificial sweeteners.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew W Moran,Miran A Al-Rammahi,Daleep K Arora,Daniel J Batchelor,Erin A Coulter,Kristian Daly,Catherine Ionescu,David Bravo,Soraya P Shirazi-Beechey
View all publications
style
left-column
style
left-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。
style
left-column
style
right-column

关联产品

Select an associated product type
Alternative Product
Primary Antibodies

AB3505

Anti-AANAT antibody

primary-antibodies

aanat-antibody-ab3505

0

(1 reviews)

Alternative Product
Primary Antibodies

AB3506

Anti-AANAT antibody

primary-antibodies

aanat-antibody-ab3506

0

(0 reviews)

style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com