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Anti-AANAT抗体(ab3505)

Reviews (1)Q&A (1)References (12)

Key features and details

  • Rabbit polyclonal to AANAT
  • Suitable for: IHC-P, WB
  • Reacts with: Rat
  • Isotype: IgG

概述

  • 产品名称

    Anti-AANAT抗体
    参阅全部 AANAT 一抗

  • 描述

    兔多克隆抗体to AANAT

  • 宿主

    Rabbit

  • 经测试应用

    适用于: IHC-P, WBmore details

  • 种属反应性

    与反应: Rat
    预测可用于: Mouse, Chicken, Hamster, Human, Chimpanzee, Rhesus monkey

  • 免疫原

    Synthetic peptide corresponding to Mouse AANAT aa 1-100 (N terminal).
    Database link: O88816

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...

  • 纯度

    Immunogen affinity purified

  • 纯化说明

    Total anti-N-terminal AA-NAT-specific IgG was affinity purified on resin-coupled non-phosphopeptide. Phospho-specific amino-terminal IgG was subsequently isolated on resin-coupled phosphopeptide.

  • Primary antibody说明

    Pineal gland serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase, AA-NAT) is an ~23 kDa protein which is the rate-limiting enzyme in melatonin synthesis. Melatonin serves as the hormonal signal of the daily light/dark cycle, or body clock. The nocturnal oscillation in enzyme activity ranges by 10- to 100-fold depending on species among vertebrates. Studies have shown that AA-NAT is regulated by cAMP at both the mRNA and protein levels. AA-NAT has putative protein kinase A (PKA) sites at Thr 29 and Ser 206. Phosphorylation of AA-NAT at these residues might play a role in protein inactivation via proteasome-dependent degradation.

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab3505于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 23 kDa.

This antibody detects an ~23 kDa protein representing unphosphorylated and phosphorylated AA-NAT from rat pineal gland whole protein extract.
说明
IHC-P
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 23 kDa.

This antibody detects an ~23 kDa protein representing unphosphorylated and phosphorylated AA-NAT from rat pineal gland whole protein extract.

靶标

  • 功能

    Controls the night/day rhythm of melatonin production in the pineal gland. Catalyzes the N-acetylation of serotonin into N-acetylserotonin, the penultimate step in the synthesis of melatonin.

  • 组织特异性

    Highly expressed in pineal gland and at lower levels in the retina. Weak expression in several brain regions and in the pituitary gland.

  • 通路

    Aromatic compound metabolism; melatonin biosynthesis; melatonin from serotonin: step 1/2.

  • 疾病相关

    Delayed sleep phase syndrome

  • 序列相似性

    Belongs to the acetyltransferase family. AANAT subfamily.
    Contains 1 N-acetyltransferase domain.

  • 翻译后修饰

    cAMP-dependent phosphorylation on both N-terminal Thr-31 and C-terminal Ser-205 regulates AANAT activity by promoting interaction with 14-3-3 proteins.

  • 细胞定位

    Cytoplasm.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • AA-NAT antibody
    • Aanat antibody
    • Aralkylamine N-acetyltransferase antibody
    • Arylalkylamine N acetyltransferase antibody
    • Serotonin acetylase antibody
    • Serotonin N-acetyltransferase antibody
    • SNAT antibody
    • SNAT_HUMAN antibody
    see all

文献 (12)

发表研究结果有使用 ab3505？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab3505 被引用在 12 文献中.

  • Yang C  et al. Melatonin delays ovarian aging in mice by slowing down the exhaustion of ovarian reserve. Commun Biol 4:534 (2021). PubMed: 33958705
  • Yang M  et al. Melatonin promotes male reproductive performance and increases testosterone synthesis in mammalian Leydig cells†. Biol Reprod 104:1322-1336 (2021). PubMed: 33709108
  • Wang J  et al. Melatonin Alleviates the Suppressive Effect of Hypoxanthine on Oocyte Nuclear Maturation and Restores Meiosis via the Melatonin Receptor 1 (MT1)-Mediated Pathway. Front Cell Dev Biol 9:648148 (2021). PubMed: 33937242
  • Song L  et al. Roseburia hominis Increases Intestinal Melatonin Level by Activating p-CREB-AANAT Pathway. Nutrients 14:N/A (2021). PubMed: 35010992
  • Yi G  et al. A study of the mechanism of small-molecule soybean-protein-derived peptide supplement to promote sleep in a mouse model. RSC Adv 10:11264-11273 (2020). PubMed: 35495343
View all Publications for this product

客户评价及客户问答

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1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-AANAT antibody

 Inconclusive
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (intestine (checked with AANAT mRNA expression))
Gel Running Conditions
Non-reduced Denaturing
Loading amount
15 µg
Specification
intestine (checked with AANAT mRNA expression)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jan 06 2023

Question

The customer has tested the secondary with other primary antibody, it's proved to work well. and she has got postive band on the same membrane when using actin antibody(loading control?, this proved that the load amount of the protein is enough. about block with 5%BSA, the customer and we think it shouldn't affect the result so much, so the custmer wouldn't like to retest it again. the cell lysate from rat pineal cells the customer used are from primary cell culture and the cells were collected after cultured for 10 days. the cells has been proved still show pineal cell characteristics by detected with 5-HT and melatonin in the culture supernate.

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Answer

Regarding the feedback on the secondary and the loading control working well, this is very reassuring and tells us the problem is not due to these. As the customer is sure the cells she is investigating are still pineal and should definitely express Serotonin N-acetyltransferase we would indeed expect a band at the correct size in her samples. I have read through Tanya's recommendations and agree with her that blocking can make a big difference. In our laboratory we try various blocking agents and see significant changes in the binding ability of antibodies. We have had instances where milk has prevented the binding of an antibody but where BSA gave very a good signal. Please pass on all our technical recommendations to our customers as if they do not follow our recommendations we will not offer replacements or refunds if the antibody was supposed to work as described on the datasheet. I would therefore appreciate you asking the customer to try with a different blocking agent of 5% BSA for 1hr (incubating the antibody in TBST only). If the customer still experiences problems with this modification please do not hesitate to contact us again,

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