AB134971

重组Anti-AAK1抗体[EPR5126(2)]

Anti-AAK1 antibody [EPR5126(2)]

RabMAb
Recombinant
了解详情

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(3 Publications)

Rabbit Recombinant Monoclonal AAK1 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications.

查看别名

KIAA1048, AAK1, AP2-associated protein kinase 1, Adaptor-associated kinase 1

2 Images

Unknown

Western blot - Anti-AAK1 antibody [EPR5126(2)] (AB134971)

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Western blot - Anti-AAK1 antibody [EPR5126(2)] (ab134971) at 1/1000 dilution

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HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 20ug

Secondary

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Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/2000 dilution

Predicted band size: 104 kDa

Observed band size: 140 kDa

false

Unknown

Western blot - Anti-AAK1 antibody [EPR5126(2)] (AB134971)

All lanes:

Western blot - Anti-AAK1 antibody [EPR5126(2)] (ab134971) at 1/10000 dilution

All lanes:

His-tagged human AAK1 recombinant protein (aa 1-365) 10ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 104 kDa

Observed band size: 42 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-AAK1 antibody [EPR5126(2)] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR5126(2)

亚型

IgG

不含载体蛋白

反应种属

Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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产品详情

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

AAK1 also known as adaptor-associated kinase 1 is a protein kinase with a molecular weight of 88 kDa. It plays a significant role in the regulation of clathrin-mediated endocytosis. AAK1 phosphorylates the mu2 subunit of the AP2 complex which is critical for the recruitment of clathrin to membranes. This protein is expressed in various tissues with high levels noted in the brain and spinal cord. AAK1 also participates in the modulation of neurotransmitter receptors and synaptic vesicle function.

Biological function summary

AAK1 functions as part of the AP2 clathrin adaptor complex which is essential for the internalization of cargo at the plasma membrane. Its activity is important for maintaining proper cellular uptake of nutrients receptors and signaling molecules. In neurons AAK1 regulates the cycle of synaptic vesicles impacting neurotransmission by influencing vesicle release and recycling. It has been suggested that AAK1 also contributes to the modulation of dendritic spine morphology through its kinase activity.

Pathways

AAK1 participates in endocytosis and intracellular trafficking pathways. It is directly involved in the clathrin-mediated endocytic route interacting with other proteins such as clathrin and dynamin. This association with clathrin provides a mechanism for cargo selection and membrane curvature linking AAK1 to the broader system of intracellular transport. In addition AAK1 connects to MAPK signaling through potential cross-talk mechanisms highlighting its role in cellular signaling regulation.

AAK1 has connections to neurodegeneration and cancer. In diseases like Alzheimer's abnormal function or expression of AAK1 can affect synaptic activity potentially exacerbating memory loss and cognitive decline. AAK1 also shows altered expression patterns in some cancers suggesting a role in oncogenic pathways. Its interaction with proteins like ALK and Ret may influence cell migration and proliferation reflecting AAK1's impact on disease states.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis (PubMed : 11877457, PubMed : 11877461, PubMed : 12952931, PubMed : 14617351, PubMed : 17494869, PubMed : 25653444). Isoform 1 and isoform 2 display similar levels of kinase activity towards AP2M1 (PubMed : 17494869). Preferentially, may phosphorylate substrates on threonine residues (PubMed : 11877457, PubMed : 18657069). Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes (PubMed : 12952931). Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes (PubMed : 18657069). Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity (PubMed : 21464124).. (Microbial infection) By regulating clathrin-mediated endocytosis, AAK1 plays a role in the entry of hepatitis C virus as well as for the lifecycle of other viruses such as Ebola and Dengue.

See full target information AAK1

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Antiviral research 204:105367 PubMed35738348

2022

Numb-associated kinases are required for SARS-CoV-2 infection and are cellular targets for antiviral strategies.

Applications

Unspecified application

Species

Unspecified reactive species

Marwah Karim,Sirle Saul,Luca Ghita,Malaya Kumar Sahoo,Chengjin Ye,Nishank Bhalla,Chieh-Wen Lo,Jing Jin,Jun-Gyu Park,Belén Martinez-Gualda,Michael Patrick East,Gary L Johnson,Benjamin A Pinsky,Luis Martinez-Sobrido,Christopher R M Asquith,Aarthi Narayanan,Steven De Jonghe,Shirit Einav

Viruses 12: PubMed31905947

2019

The Serine/Threonine Kinase AP2-Associated Kinase 1 Plays an Important Role in Rabies Virus Entry.

Applications

Unspecified application

Species

Unspecified reactive species

Chong Wang,Jinliang Wang,Lei Shuai,Xiao Ma,Hailin Zhang,Renqiang Liu,Weiye Chen,Xijun Wang,Jinying Ge,Zhiyuan Wen,Zhigao Bu

The Journal of clinical investigation 127:1338-1352 PubMed28240606

2017

Anticancer kinase inhibitors impair intracellular viral trafficking and exert broad-spectrum antiviral effects.

Applications

Unspecified application

Species

Unspecified reactive species

Elena Bekerman,Gregory Neveu,Ana Shulla,Jennifer Brannan,Szu-Yuan Pu,Stanley Wang,Fei Xiao,Rina Barouch-Bentov,Russell R Bakken,Roberto Mateo,Jennifer Govero,Claude M Nagamine,Michael S Diamond,Steven De Jonghe,Piet Herdewijn,John M Dye,Glenn Randall,Shirit Einav

View all publications

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重组Anti-AAK1抗体[EPR5126(2)]