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Anti-AAK1抗体(ab59740)

Submit a review Q&A (1)References (1)
Western blot - Anti-AAK1 antibody (ab59740)

    Key features and details

    • Rabbit polyclonal to AAK1
    • Suitable for: WB
    • Reacts with: Mouse, Rat
    • Isotype: IgG

    概述

    • 产品名称

      Anti-AAK1抗体
      参阅全部 AAK1 一抗

    • 描述

      兔多克隆抗体to AAK1

    • 宿主

      Rabbit

    • 经测试应用

      适用于: WBmore details

    • 种属反应性

      与反应: Mouse, Rat

    • 免疫原

      Synthetic peptide surrounding amino acid 850 of human AAK1.

    • 阳性对照

      • Mouse tissue lysate and rat kidney lysate.

    • 常规说明

      This product is manufactured by BioVision, an Abcam company and was previously called 3810 AAK1 Antibody. 3810-100 is the same size as the 100 µg size of ab59740.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab59740于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB
    Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 94 kDa).
    说明
    WB
    Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 94 kDa).

    靶标

    • 功能

      Phosphorylates the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2). May play a role in regulating aspects of clathrin-mediated endocytosis.

    • 序列相似性

      Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
      Contains 1 protein kinase domain.

    • 翻译后修饰

      Autophosphorylated.

    • 细胞定位

      Membrane. Cell membrane. Membrane > clathrin-coated pit. Active when found in clathrin-coated pits at the plasma membrane.
    • Information by UniProt

    • 数据库链接

      • 别名

        • AAK 1 antibody
        • Aak1 antibody
        • AAK1_HUMAN antibody
        • Adaptor associated kinase 1 antibody
        • Adaptor-associated kinase 1 antibody
        • AP2 associated kinase 1 antibody
        • AP2 associated protein kinase 1 antibody
        • AP2-associated protein kinase 1 antibody
        • DKFZp686F03202 antibody
        • DKFZp686K16132 antibody
        • FLJ23712 antibody
        • FLJ25931 antibody
        • FLJ31060 antibody
        • FLJ42882 antibody
        • FLJ45252 antibody
        • KIAA1048 antibody
        • MGC138170 antibody
        • MGC164568 antibody
        • MGC164570 antibody
        see all

      图片

      文献 (1)

      发表研究结果有使用 ab59740？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab59740 被引用在 1 文献中.

      • Hu J  et al. Allele-specific silencing of mutant huntingtin and ataxin-3 genes by targeting expanded CAG repeats in mRNAs. Nat Biotechnol 27:478-84 (2009). PubMed: 19412185

      客户评价及客户问答

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      Question

      Non-specific bands in Western blot with ab59740.

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      Answer

      Thanks for your phone call and for letting us know about the trouble with ab59740. As we discussed, I am sending a free of charge vial of ab77082 as a replacement, which should arrive late next week. If you have any Western blot images using ab59740, they will be very helpful in our investigation of the problem. Please let me know if you have any questions or if there is anything else that we can do for you.

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      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com