重组Anti-AACT抗体[EPR14117(B)] (ab180492)
![Western blot - Anti-AACT antibody [EPR14117(B)] (ab180492)](https://www.abcam.cn/ps/products/180/ab180492/Images/ab180492-211947-anti-aact-antibody-epr14117b-western-blot.jpg "Western blot - Anti-AACT antibody [EPR14117(B)] (ab180492)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14117(B)] to AACT
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-AACT抗体[EPR14117(B)]
参阅全部 AACT 一抗 -
描述
兔单克隆抗体[EPR14117(B)] to AACT -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, IPmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Human plasma and colon cancer lysates; Human liver and placenta tissues.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR14117(B) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab180492于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
| WB |
1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 47 kDa).
|
|
| IP |
1/70.
|
| 说明 |
|---|
|
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
WB
1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 47 kDa). |
|
IP
1/70. |
靶标
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功能
Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2. -
组织特异性
Plasma. Synthesized in the liver. Like the related alpha-1-antitrypsin, its concentration increases in the acute phase of inflammation or infection. Found in the amyloid plaques from the hippocampus of Alzheimer disease brains. -
疾病相关
Defects in SERPINA3 may be a cause of chronic obstructive pulmonary disease (COPD) [MIM:107280]. -
序列相似性
Belongs to the serpin family. -
结构域
The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 12 Human
- Omim: 107280 Human
- SwissProt: P01011 Human
- Unigene: 534293 Human
- Unigene: 710488 Human
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别名
- SERPINA3 antibody
- AACT antibody
- AACT_HUMAN antibody
see all
图片
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Western blot - Anti-AACT antibody [EPR14117(B)] (ab180492)Anti-AACT antibody [EPR14117(B)] (ab180492) at 1/10000 dilution + Human plasma lysate at 20 µg
Secondary
Rabbit TrueBlot®:Anti-Rabbit IgG HRP at 1/1000 dilution
Predicted band size: 47 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted? -
![Western blot - Anti-AACT antibody [EPR14117(B)] (ab180492)](https://www.abcam.cn/ps/products/180/ab180492/Images/ab180492-211948-anti-aact-antibody-epr14117b-western-blot.jpg)
Western blot - Anti-AACT antibody [EPR14117(B)] (ab180492)Anti-AACT antibody [EPR14117(B)] (ab180492) at 1/2000 dilution + Human colon cancer tissue lysate at 20 µg
Secondary
Rabbit TrueBlot®:Anti-Rabbit IgG HRP at 1/1000 dilution
Predicted band size: 47 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACT antibody [EPR14117(B)] (ab180492)](https://www.abcam.cn/ps/products/180/ab180492/Images/ab180492-211949-anti-aact-antibody-epr14117b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACT antibody [EPR14117(B)] (ab180492)Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling AACT with ab180492 at 1/500 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACT antibody [EPR14117(B)] (ab180492)](https://www.abcam.cn/ps/products/180/ab180492/Images/ab180492-211951-anti-aact-antibody-epr14117b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACT antibody [EPR14117(B)] (ab180492)Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling AACT with ab180492 at 1/500 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
-
![Immunoprecipitation - Anti-AACT antibody [EPR14117(B)] (ab180492)](https://www.abcam.cn/ps/products/180/ab180492/Images/ab180492-211952-anti-aact-antibody-epr14117b-immunoprecipitation.jpg)
Immunoprecipitation - Anti-AACT antibody [EPR14117(B)] (ab180492)Western blot analysis of Human plasma immunoprecipitated using ab180492 at 1/70 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate was used as secondary antibody, at 1/1000 dilution.
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![Anti-AACT antibody [EPR14117(B)] (ab180492)](https://www.abcam.cn/ps/products/180/ab180492/Images/ab180492-6-benefits-of-recombinant-antibodies.png)
Anti-AACT antibody [EPR14117(B)] (ab180492)
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (3)
ab180492 被引用在 3 文献中.
- Fang W et al. Serpina3n/serpina3 alleviates cyclophosphamide-induced interstitial cystitis by activating the Wnt/β-catenin signal. Int Urol Nephrol 55:3065-3075 (2023). PubMed: 37594700
- Fan Y et al. Single-cell analysis reveals region-heterogeneous responses in rhesus monkey spinal cord with complete injury. Nat Commun 14:4796 (2023). PubMed: 37558705
- Li Y et al. SERPINA3 induced by astroglia/microglia co-culture facilitates glioblastoma stem-like cell invasion. Oncol Lett 15:285-291 (2018). PubMed: 29399139