Anti-AACT抗体(ab9374)
Key features and details
- Rabbit polyclonal to AACT
- Suitable for: IHC-P, Sandwich ELISA, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-AACT抗体
参阅全部 AACT 一抗 -
描述
兔多克隆抗体to AACT -
宿主
Rabbit -
经测试应用
适用于: IHC-P, Sandwich ELISA, WBmore details -
种属反应性
与反应: Human
预测可用于: Mammals
不与反应:
Mouse -
免疫原
Full length native protein (purified) corresponding to AACT. Alpha-1-antichymotrypsin isolated from human serum.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.3
Constituents: PBS, 0.01425% Magnesium chloride, 0.00154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.01% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
轻链类型
unknown -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/25 - 1/50. ABC method.
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| Sandwich ELISA |
Use at an assay dependent concentration.
For sandwich ELISA, use this antibody as Detection at an assay dependent concentration with Mouse monoclonal to AACT (ab54693) as Capture. |
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| WB |
Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 48 kDa).
Assay Dependent |
| 说明 |
|---|
|
IHC-P
1/25 - 1/50. ABC method. |
|
Sandwich ELISA
Use at an assay dependent concentration. For sandwich ELISA, use this antibody as Detection at an assay dependent concentration with Mouse monoclonal to AACT (ab54693) as Capture. |
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WB
Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 48 kDa). Assay Dependent |
靶标
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功能
Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2. -
组织特异性
Plasma. Synthesized in the liver. Like the related alpha-1-antitrypsin, its concentration increases in the acute phase of inflammation or infection. Found in the amyloid plaques from the hippocampus of Alzheimer disease brains. -
疾病相关
Defects in SERPINA3 may be a cause of chronic obstructive pulmonary disease (COPD) [MIM:107280]. -
序列相似性
Belongs to the serpin family. -
结构域
The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 12 Human
- Omim: 107280 Human
- SwissProt: P01011 Human
- Unigene: 534293 Human
- Unigene: 710488 Human
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别名
- SERPINA3 antibody
- AACT antibody
- AACT_HUMAN antibody
see all
图片
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Western blot - Anti-AACT antibody (ab9374)All lanes : Anti-AACT antibody (ab9374) at 1 µg/ml
Lane 1 : Human spinal cord tissue lysate - total protein (ab29188)
Lane 2 : Human colon tissue lysate - total protein (ab30051)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 48 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted? -
Sandwich ELISA - Anti-AACT antibody (ab9374)Standard Curve for AACT (Analyte: AACT protein (Human) (ab80517) ); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal to AACT (ab54693) at 5µg/ml and Detector Antibody Rabbit polyclonal to AACT (ab9374) at 0.5µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACT antibody (ab9374)Human normal skin. Staining is mainly membrane-bound and cytoplasmic. Left panel: with primary antibody at 2 ug/ml. Right panel: negative control. Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for rabbit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required. -
Western blot - Anti-AACT antibody (ab9374)
实验方案
数据表及文件
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Datasheet download
文献 (1)
ab9374 被引用在 1 文献中.
- Martin-Rojas T et al. A clinical perspective on the utility of alpha 1 antichymotrypsin for the early diagnosis of calcific aortic stenosis. Clin Proteomics 14:12 (2017). WB, IHC . PubMed: 28439213
