Anti-A-Raf (phospho S299)抗体(ab112053)
Key features and details
- Rabbit polyclonal to A-Raf (phospho S299)
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-A-Raf (phospho S299)抗体
参阅全部 A-Raf 一抗 -
描述
兔多克隆抗体to A-Raf (phospho S299) -
宿主
Rabbit -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide corresponding to Human A-Raf (phospho S299).
Database link: P10398 -
阳性对照
- Human colon carcinoma tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.88% Sodium chloride, 50% Glycerol (glycerin, glycerine), 49% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab112053 was affinity-purified from Rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab112053于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. -
组织特异性
Predominantly in urogenital tissues. -
序列相似性
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain.
Contains 1 RBD (Ras-binding) domain. - Information by UniProt
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数据库链接
- Entrez Gene: 369 Human
- Entrez Gene: 11836 Mouse
- Entrez Gene: 64363 Rat
- Omim: 311010 Human
- SwissProt: P10398 Human
- SwissProt: P04627 Mouse
- SwissProt: P14056 Rat
- Unigene: 446641 Human
see all -
别名
- A raf 1 antibody
- A Raf proto oncogene serine/threonine protein kinase antibody
- ARAF 1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A-Raf (phospho S299) antibody (ab112053)
ab112053 at 1/50 dilution staining A-Raf in Paraffin-embedded Human colon carcinoma tissue by Immunohistochemistry. The image on the right is treated with the synthesized phospho peptide.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab112053 被引用在 1 文献中.
- Hou J et al. Caudatin blocks the proliferation, stemness and glycolysis of non-small cell lung cancer cells through the Raf/MEK/ERK pathway. Pharm Biol 60:764-773 (2022). PubMed: 35387566