Anti-8-Hydroxy-2'-deoxyguanosine抗体[N45.1] (ab48508)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-8-Hydroxy-2'-deoxyguanosine antibody [N45.1] (ab48508)](https://www.abcam.cn/ps/products/48/ab48508/Images/ab48508-259999-anti-8-hydroxy-2-deoxyguanosine-antibody-n451-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-8-Hydroxy-2'-deoxyguanosine antibody [N45.1] (ab48508)")
Key features and details
- Mouse monoclonal [N45.1] to 8-Hydroxy-2'-deoxyguanosine
- Suitable for: IHC-P
- Reacts with: Species independent
- Isotype: IgG1
概述
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产品名称
Anti-8-Hydroxy-2'-deoxyguanosine抗体[N45.1] -
描述
小鼠单克隆抗体[N45.1] to 8-Hydroxy-2'-deoxyguanosine -
宿主
Mouse -
特异性
Nineteen analogues of 8-Hydroxy-2'-deoxyguanosine {guanosine (G), 7-methyl-G, 6-SH-G, 8-bromo-G, dA, dC, dT, dI, dU, dG, O6-methyl-dG, 8-OHdA, guanine (Gua), O6-methyl-Gua, 8-OHGua, uric acid, Urea, creatine, creatinine} demonstrate no cross-reactivity. Only 8-sulfhydryl-G and 8-OHG demonstrate minimal cross-reactivity (less than 1%). -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Species independent -
免疫原
Chemical/ Small Molecule: 8-Hydroxy-2'-deoxyguanosine conjugated Keyhole Limpet Hemocyanin
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阳性对照
- Epidermis from hairless mouse treated for 4 weeks with chronic UVB irradiation, or kidney treated with Fe-NTA.
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常规说明
This product was changed from ascites to tissue culture supernatant on 27th February 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Constituent: 0.41% PBS -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
N45.1 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab48508于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (3) |
Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
For mouse on mouse staining we recommend uisng MOM kit - ab127055. |
| 说明 |
|---|
|
IHC-P
Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. For mouse on mouse staining we recommend uisng MOM kit - ab127055. |
靶标
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相关性
8-hydroxy-2'-deoxyguanosine is a biomarker of oxidative DNA damage. -
别名
- 8 OHdG antibody
- 8-OHdG antibody
- 8OHdG antibody
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-8-Hydroxy-2'-deoxyguanosine antibody [N45.1] (ab48508)Image from Wu L et al., PLoS ONE 8(4): e60262. Fig 6 doi:10.1371/journal.pone.0060262 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
De-paraffinized and dehydrated sections were treated in a microwave oven at low power for 10 min in 0.01 M sodium citrate buffer (pH 6.0) to retrieve antigen, and inactivated endogenous peroxidase using 3% hydrogen peroxide in methanol for 10 min, and blocked with blocking solution for 1 hour at room temperature. Sections were then incubated with 1∶20 dilution of mouse monoclonal antibody to 8-Hydroxy-2'-deoxyguanosine (ab48508) overnight at 4°C. For detection, a biotinylated anti-mouse and HRP-labeled Streptavidin, and staining with DAB was used.
Immunohistochemical staining of nitrotyrosine (A) and 8-hydroxy-2′-deoxyguanosine (B) was performed on 5 rats randomly chosen from each group (ND: chow diet group, HFD: High fat diet, HFD-QH: High fat diet treated with Qing Huo Yi Hao" (QHYH)). An islet is encircled in each quandrant. Black arrows point to islets of Langerhans (Original magnification ×200). Percentage of immunohistochemial positive staining area in islet out of the area of corresponding islet (C, D) was presented. Values and bars represent means±SEM. * and ** denote P<0.05 and P<0.001 versus group ND.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-8-Hydroxy-2'-deoxyguanosine antibody [N45.1] (ab48508)](https://www.abcam.cn/ps/products/48/ab48508/Images/ab48508-260006-anti-8-hydroxy-2-deoxyguanosine-antibody-n451-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-8-Hydroxy-2'-deoxyguanosine antibody [N45.1] (ab48508)This image is courtesy of an Abreview submitted by Rudolf Jungab48508 staining 8-Hydroxy-2'-deoxyguanosine in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a TE buffer. Samples were incubated with primary antibody (1/50) for 30 mins at 20°C. A HRP-conjugated Goat anti-mouse IgG was used as the secondary antibody.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-8-Hydroxy-2'-deoxyguanosine antibody [N45.1] (ab48508)](https://www.abcam.cn/ps/products/48/ab48508/Images/ab48508-61017-anti-8-hydroxy-2-deoxyguanosine-antibody-n451-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-8-Hydroxy-2'-deoxyguanosine antibody [N45.1] (ab48508)Ab48508, at a concentration of 5 µg/ml, staining 8-Hydroxy-2'-deoxyguanosine in epidermis from hairless mouse after 4 weeks of treatment by chronic UVB irradiation.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (125)
ab48508 被引用在 125 文献中.
- Lohakul J et al. Mitochondria-Targeted Hydrogen Sulfide Delivery Molecules Protect Against UVA-Induced Photoaging in Human Dermal Fibroblasts, and in Mouse Skin In Vivo. Antioxid Redox Signal 36:1268-1288 (2022). PubMed: 34235951
- Qiu D et al. NAD(P)H: quinone oxidoreductase 1 attenuates oxidative stress and apoptosis by regulating Sirt1 in diabetic nephropathy. J Transl Med 20:44 (2022). PubMed: 35090502
- Bosso G et al. Early differential responses elicited by BRAFV600E in adult mouse models. Cell Death Dis 13:142 (2022). PubMed: 35145078
- Li T et al. Kansl1 haploinsufficiency impairs autophagosome-lysosome fusion and links autophagic dysfunction with Koolen-de Vries syndrome in mice. Nat Commun 13:931 (2022). PubMed: 35177641
- Chen JW et al. mito-TEMPO Attenuates Oxidative Stress and Mitochondrial Dysfunction in Noise-Induced Hearing Loss via Maintaining TFAM-mtDNA Interaction and Mitochondrial Biogenesis. Front Cell Neurosci 16:803718 (2022). PubMed: 35210991