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Tags & Cell Markers Epitope Tags His Tag

Anti-6X His tag®抗体[HIS.H8] (ab18184)

  • Datasheet
  • SDS
Reviews (32)Q&A (10)References (356)

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Immunoprecipitation - Anti-6X His tag® antibody [HIS.H8] (ab18184)
  • Western blot - Anti-6X His tag® antibody [HIS.H8] (ab18184)
  • Immunocytochemistry/ Immunofluorescence - Anti-6X His tag® antibody [HIS.H8] (ab18184)
  • Western blot - Anti-6X His tag® antibody [HIS.H8] (ab18184)
  • Immunocytochemistry/ Immunofluorescence - Anti-6X His tag® antibody [HIS.H8] (ab18184)

Key features and details

  • Mouse monoclonal [HIS.H8] to 6X His tag®
  • Suitable for: ICC/IF, ELISA, Dot blot, WB, IP
  • Reacts with: Species independent
  • Isotype: IgG2b

Conjugates logo Related conjugates and formulations

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概述

  • 产品名称

    Anti-6X His tag®抗体[HIS.H8]
    参阅全部 6X His tag® 一抗
  • 描述

    小鼠单克隆抗体[HIS.H8] to 6X His tag®
  • 宿主

    Mouse
  • 特异性

    Recognizes His-tagged recombinant proteins or His-tagged proteins overexpressed in cells.

    ab18184 reacts to recombinant proteins containing the 6X His tag® or 10X His tag® fused to either the amino or carboxy terminus.

  • 经测试应用

    适用于: ICC/IF, ELISA, Dot blot, WB, IPmore details
  • 种属反应性

    与反应: Species independent
  • 免疫原

    Synthetic peptide corresponding to 6X His tag®.

  • 常规说明

    This product was changed from ascites to tissue culture supernatant on 5th February 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    HIS-TAG® is a trademark of EMD Biosciences, Inc.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 纯化说明

    Purified from TCS by Protein A affinity chromatography
  • 克隆

    单克隆
  • 克隆编号

    HIS.H8
  • 同种型

    IgG2b
  • 研究领域

    • Tags & Cell Markers
    • Epitope Tags
    • His Tag
    • Signal Transduction
    • Antibodies
    • 6x his tag®

相关产品

  • Alternative Versions

    • Biotin Anti-6X His tag® antibody [HIS.H8] (ab173828)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
  • Isotype control

    • Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab18184于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF (3)
1/200.
ELISA (1)
Use at an assay dependent concentration.
Dot blot
Use at an assay dependent concentration.
WB (21)
1/1000.
IP (5)
1/200.
说明
ICC/IF
1/200.
ELISA
Use at an assay dependent concentration.
Dot blot
Use at an assay dependent concentration.
WB
1/1000.
IP
1/200.

靶标

  • 相关性

    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. His-tags can be fused to the amino- or carboxy- termini of proteins in transfected or transformed cells.
  • 细胞定位

    Depends upon the localization of the parent protein tagged with hexahistidine.
  • 别名

    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

图片

  • Immunoprecipitation - Anti-6X His tag® antibody [HIS.H8] (ab18184)
    Immunoprecipitation - Anti-6X His tag® antibody [HIS.H8] (ab18184)This image is courtesy of an Abreview submitted by Mr Chris Tracy

    Immunoprecipitation of His/FLAG tagged PDCD5 using ab18184 at 1/100 dilution.

    Lane 1 : MW Ladder (from top 120, 85, 50, 34, 25, 19 kDa)
    Lanes 2-5 : Immunoprecipitation of His/FLAG tagged PDCD5 using ab18184 from mixture of ~50 ng purified His/FLAG tagged PDCD5 and 15 µL RRL in 300 µL buffer.
    Lanes 6-9 : Purified PDCD5 from E. coli with ~60-70 ng protein.

    The lowest band in each lane is ~ 20 kDa and is PDCD5. The upper bands in lanes 2-5 are the heavy chain (~50 kDa, runs as doublet) and light chain (~25 or 26 kDa).

    Western Blot was performed with a mouse anti-FLAG primary antibody at 1/2000 dilution. An IRDye®800CW-conjugated goat anti-mouse IgG was used as a secondary antibody.

    See Abreview

  • Western blot - Anti-6X His tag® antibody [HIS.H8] (ab18184)
    Western blot - Anti-6X His tag® antibody [HIS.H8] (ab18184)This image is courtesy of an Abreview submitted by Vladimir Milenkovic
    All lanes : Anti-6X His tag® antibody [HIS.H8] (ab18184) at 1/5000 dilution

    Lane 1 : ARPE-19 whole cell lysate - non-transfected
    Lane 2 : ARPE-19 whole cell lysate - transfected with rRab27a-His

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-mouse IgG polyclonal at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 5 minutes


    Blocked with 5% milk for 30 minutes at 25°C.

    Incubated with the primary antibody for 16 hours at 4°C in 1X PBS/Tween, 5% milk.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-6X His tag® antibody [HIS.H8] (ab18184)
    Immunocytochemistry/ Immunofluorescence - Anti-6X His tag® antibody [HIS.H8] (ab18184)

    LEFT: untransfected control; RIGHT: anti-His (in red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (in blue).

  • Western blot - Anti-6X His tag® antibody [HIS.H8] (ab18184)
    Western blot - Anti-6X His tag® antibody [HIS.H8] (ab18184)This image is courtesy of an Abreview submitted by Rachel Loh
    All lanes : Anti-6X His tag® antibody [HIS.H8] (ab18184) at 1/1000 dilution

    Lane 1 : 6x His-tagged protein expressed in BL21 (pET15b) at 1 µg
    Lane 2 : 6x His-tagged protein expressed in BL21 (pET15b) at 0.1 µg

    Secondary
    All lanes : IRDye® 800CW goat anti-mouse IgG (H+L) polyclonal at 1/5000 dilution

    Performed under reducing conditions.

    Exposure time: 5 minutes

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-6X His tag® antibody [HIS.H8] (ab18184)
    Immunocytochemistry/ Immunofluorescence - Anti-6X His tag® antibody [HIS.H8] (ab18184)This image is courtesy of an anonymous abreview.
    ab18184 at a 1/1000 dilution staining 6X His tag in transfected human HEK293 cells by Immunocytochemistry/ Immunofluorescence incubated for 12 hours at 22°C. PFA fixed. Permeabilized using 0.1% Triton X-100 in PBS. Blocked using 3% BSA for 1 hour at 22°C. Secondary used at a 1/1000 dilution polyclonal Goat anti-mouse IgG (H+L) conjugated to Alexa Fluor 488 (green). DAPI staining (blue).

    See Abreview

实验方案

  • Immunoprecipitation protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (356)

发表研究结果有使用 ab18184?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab18184 被引用在 356 文献中.

  • Aoki H  et al. Thymidine Kinase 2 and Mitochondrial Protein COX I in the Cerebellum of Patients with Spinocerebellar Ataxia Type 31 Caused by Penta-nucleotide Repeats (TTCCA)n. Cerebellum 22:70-84 (2023). PubMed: 35084690
  • Saville JW  et al. Structural analysis of receptor engagement and antigenic drift within the BA.2 spike protein. Cell Rep 42:111964 (2023). PubMed: 36640338
  • Stevens AJ  et al. Programming multicellular assembly with synthetic cell adhesion molecules. Nature 614:144-152 (2023). PubMed: 36509107
  • Zhang C  et al. Centrosomal protein 120 promotes centrosome amplification and gastric cancer progression via USP54-mediated deubiquitination of PLK4. iScience 26:105745 (2023). PubMed: 36590171
  • Banijamali E  et al. RNA:RNA interaction in ternary complexes resolved by chemical probing. RNA 29:317-329 (2023). PubMed: 36617673
View all Publications for this product

客户评价及客户问答

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ELISA abreview for Anti-6X His tag® antibody [HIS.H8]

Excellent
Abreviews
Abreviews
Application
ELISA
Sample
Human Recombinant protein (purified recombinant protein)
Specification
purified recombinant protein
Blocking step
2% BSA and 0.05% Tween-20 in PBS as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Type
Direct
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Sebastian Kalamajski

Verified customer

提交于 Jun 12 2012

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