Anti-6X His tag®抗体[HIS-1] (ab49936)
Key features and details
- Mouse monoclonal [HIS-1] to 6X His tag®
- Suitable for: Flow Cyt (Intra), WB, Dot blot, ELISA, ICC/IF
- Reacts with: Species independent
- Isotype: IgG2a
Related conjugates and formulations
概述
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产品名称
Anti-6X His tag®抗体[HIS-1]
参阅全部 6X His tag® 一抗 -
描述
小鼠单克隆抗体[HIS-1] to 6X His tag® -
宿主
Mouse -
特异性
ab49939 preferentially reacts to recombinant proteins containing the 6X His tag® fused to amino terminus of fusion proteins.
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经测试应用
适用于: Flow Cyt (Intra), WB, Dot blot, ELISA, ICC/IFmore details -
种属反应性
与反应: Species independent -
免疫原
Recombinant full length protein corresponding to 6X His tag®.
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常规说明
This product was changed from ascites to tissue culture supernatant on 25th October 2016. The following lot(s) is/are from ascites and is still in stock as of 25th October 2016 - GR224242. Lot numbers higher than GR224242 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
HIS-TAG® is a trademark of EMD Biosciences, Inc.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Proprietary Purification -
纯化说明
Purified from Tissue culture supernatant. -
克隆
单克隆 -
克隆编号
HIS-1 -
同种型
IgG2a -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab49936于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
Use at an assay dependent concentration.
|
|
| WB |
1/3000.
|
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| Dot blot |
Use at an assay dependent concentration.
|
|
| ELISA |
Use at an assay dependent concentration.
|
|
| ICC/IF |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Flow Cyt (Intra)
Use at an assay dependent concentration. |
|
WB
1/3000. |
|
Dot blot
Use at an assay dependent concentration. |
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ELISA
Use at an assay dependent concentration. |
|
ICC/IF
Use at an assay dependent concentration. |
靶标
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相关性
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. His-tags can be fused to the amino- or carboxy- termini of proteins in transfected or transformed cells. -
细胞定位
Depends upon the localization of the parent protein tagged with hexahistidine. -
别名
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
see all
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab49936 被引用在 3 文献中.
- Li T et al. SICKLE represses photomorphogenic development of Arabidopsis seedlings via HY5- and PIF4-mediated signaling. J Integr Plant Biol 64:1706-1723 (2022). PubMed: 35848532
- Allen ER et al. A Protective Monoclonal Antibody Targets a Site of Vulnerability on the Surface of Rift Valley Fever Virus. Cell Rep 25:3750-3758.e4 (2018). PubMed: 30590046
- Burgos JM & Schmitt MP The ChrSA and HrrSA Two-Component Systems Are Required for Transcriptional Regulation of the hemA Promoter in Corynebacterium diphtheriae. J Bacteriol 198:2419-30 (2016). PubMed: 27381918