Anti-6X His tag®抗体[AD1.1.10] (ab15149)
Key features and details
- Mouse monoclonal [AD1.1.10] to 6X His tag®
- Suitable for: ICC/IF, IHC-Fr, IP, WB
- Reacts with: Species independent
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
Anti-6X His tag®抗体[AD1.1.10]
参阅全部 6X His tag® 一抗 -
描述
小鼠单克隆抗体[AD1.1.10] to 6X His tag® -
宿主
Mouse -
经测试应用
适用于: ICC/IF, IHC-Fr, IP, WBmore details -
种属反应性
与反应: Species independent -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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常规说明
The clone number has been updated from (3H2201) to (AD1.1.10) both clone numbers name the same antibody clone.
HIS-TAG® is a trademark of EMD Biosciences, Inc.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.58% Sodium chloride, 95% Sodium phosphate -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
Tissue cuture supernatant purified by Protein G affinity chromatography. -
Primary antibody说明
ab15149 recognises proteins containing the motif HHHHHH and is therefore of value in detecting proteins containing histidine tags. The antibody may be used to track the purification of proteins expressed in bacteria, yeast or insect cells. -
克隆
单克隆 -
克隆编号
AD1.1.10 -
骨髓瘤
NS1 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
- PerCP Anti-6X His tag® antibody [AD1.1.10] (ab117496)
- DyLight® 650 Anti-6X His tag® antibody [AD1.1.10] (ab117504)
- DyLight® 550 Anti-6X His tag® antibody [AD1.1.10] (ab117507)
- DyLight® 488 Anti-6X His tag® antibody [AD1.1.10] (ab117512)
- Biotin Anti-6X His tag® antibody [AD1.1.10] (ab15145)
- HRP Anti-6X His tag® antibody [AD1.1.10] (ab178563)
- Europium Anti-6X His tag® antibody [AD1.1.10] (ab187912)
- PE Anti-6X His tag® antibody [AD1.1.10] (ab72467)
- SureLight® APC Anti-6X His tag® antibody [AD1.1.10] (ab72579)
- Alkaline Phosphatase Anti-6X His tag® antibody [AD1.1.10] (ab81652)
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab15149于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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|
WB |
1/100 - 1/500.
|
说明 |
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ICC/IF
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
WB
1/100 - 1/500. |
靶标
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相关性
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. His-tags can be fused to the amino- or carboxy- termini of proteins in transfected or transformed cells. -
细胞定位
Depends upon the localization of the parent protein tagged with hexahistidine. -
别名
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
see all
图片
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All lanes : Anti-6X His tag® antibody [AD1.1.10] (ab15149) at 0.5 µg/ml
Lane 1 : 60 kDa 6X His tagged protein at 0.3 µg
Lane 2 : 60 kDa 6X His tagged protein at 0.1 µg
Lane 3 : 60 kDa 6X His tagged protein at 0.03 µg
Lane 4 : 60 kDa 6X His tagged protein at 0.01 µg
Lane 5 : 60 kDa 6X His tagged protein at 0.003 µg
Secondary
All lanes : Goat Anti-Mouse (HRP) at 0.04 µg/mlImaged using West Pico chemiluminescent substrate.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab15149 被引用在 9 文献中.
- Ling H et al. A sugarcane smut fungus effector simulates the host endogenous elicitor peptide to suppress plant immunity. New Phytol 233:919-933 (2022). PubMed: 34716592
- Kishimoto H et al. The lipophilic cyclic peptide cyclosporin A induces aggregation of gel-forming mucins. Sci Rep 12:6153 (2022). PubMed: 35418571
- Yi KS et al. Broader neutralization of CT-P27 against influenza A subtypes by combining two human monoclonal antibodies. PLoS One 15:e0236172 (2020). PubMed: 32726321
- Lam M et al. Misfolded proteins bind and activate death receptor 5 to trigger apoptosis during unresolved endoplasmic reticulum stress. Elife 9:N/A (2020). PubMed: 31904339
- Moreland KT et al. In vitro calcite crystal morphology is modulated by otoconial proteins otolin-1 and otoconin-90. PLoS One 9:e95333 (2014). WB . PubMed: 24748133
- Smith MH et al. Misfolded proteins induce aggregation of the lectin Yos9. J Biol Chem 289:25670-7 (2014). WB . PubMed: 25086047
- Ma L et al. Serum anti-CCNY autoantibody is an independent prognosis indicator for postoperative patients with early-stage nonsmall-cell lung carcinoma. Dis Markers 35:317-25 (2013). WB . PubMed: 24167380
- Ma Z et al. Targeting human epidermal growth factor receptor signaling with the neuregulin's heparin-binding domain. J Biol Chem 284:32108-15 (2009). ICC/IF . PubMed: 19717564
- Bergman LM et al. Role of the unique N-terminal domain of CtBP2 in determining the subcellular localisation of CtBP family proteins. BMC Cell Biol 7:35 (2006). PubMed: 16999872