Anti-6X His tag®抗体(ab9107)
Key features and details
- Chicken polyclonal to 6X His tag®
- Suitable for: WB, ELISA
- Reacts with: Species independent
- Isotype: unknown
概述
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产品名称
Anti-6X His tag®抗体
参阅全部 6X His tag® 一抗 -
描述
鸡多克隆抗体to 6X His tag® -
宿主
Chicken -
特异性
Antibody specificity was verified by ELISA against peptide conjugated to BSA (HHHHHH-BSA). A 1:8000 dilution of the antibody gave an OD=1.0 in a 15 minute reaction using Goat Anti Chicken IgY (IgG) and TMB as the substrate. Appropriate specificity controls were run. -
经测试应用
适用于: WB, ELISAmore details -
种属反应性
与反应: Species independent -
免疫原
Synthetic peptide corresponding to 6X His tag® conjugated to keyhole limpet haemocyanin.
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常规说明
HIS-TAG® is a trademark of EMD Biosciences, Inc.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.1% Sodium azide -
Concentration information loading... -
纯度
IgY fraction -
纯化说明
Antibodies were immunoaffinity purified using the peptide immobilized on a solid support. -
克隆
多克隆 -
同种型
unknown -
轻链类型
unknown -
研究领域
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 1 kDa.
This antibody was an effective at 1:1000 (1 µg/ml) dilution using an affinity purified goat anti chicken IgY (IgG) HRP. The Westerns probed with this antibody are very sharp and clear with extremely low background and no non-specific binding. |
|
| ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 1 kDa. This antibody was an effective at 1:1000 (1 µg/ml) dilution using an affinity purified goat anti chicken IgY (IgG) HRP. The Westerns probed with this antibody are very sharp and clear with extremely low background and no non-specific binding. |
|
ELISA
Use at an assay dependent concentration. |
靶标
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相关性
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. His-tags can be fused to the amino- or carboxy- termini of proteins in transfected or transformed cells. -
细胞定位
Depends upon the localization of the parent protein tagged with hexahistidine. -
别名
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
see all
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab9107 被引用在 1 文献中.
- Haas KT et al. Multitarget Immunohistochemistry for Confocal and Super-resolution Imaging of Plant Cell Wall Polysaccharides. Bio Protoc 10:e3783 (2020). PubMed: 33659438