AB5000

Anti-6X His tag® 抗体 [4D11]

Name: 6X His tag®抗体[4D11] (ab5000)| Abcam中文官网
Brand: Abcam Limited
SKU: AB5000
Price: 1386 CNY
Availability: InStock
Rating: 4 (4 reviews)

Anti-6X His tag® antibody [4D11]

Lab Essentials
了解详情

(4 Reviews)

(32 Publications)

Mouse Monoclonal 6X His tag® antibody. Suitable for IP, Flow Cyt, ELISA, Dot, WB, ICC/IF, AP and reacts with Tag samples. Cited in 32 publications.

查看别名

6 His epitope tag, HHHHHH epitope tag, HHHHHH tag, Hexa His tag, Polyhistidine Tag, 6x his, 6x-HIS

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-6X His tag® antibody [4D11] (AB5000)

ICC/IF

AbReview16566****

Immunocytochemistry/ Immunofluorescence - Anti-6X His tag® antibody [4D11] (AB5000)

ab5000 staining Human HEK 293 cells transfected with CACNB3-His by ICC/IF. Cells were PFA fixed and permeabilized in 0.5% Triton X-100 prior to blocking in 5% serum for 20 minutes at 25°C. The primary antibody (1/250 in 1% goat serum, 0.1% TX100, 1X PBS) was incubated with the sample for 16 hours at 4°C. An Alexa Fluor^® 546 conjugated goat anti-mouse antibody (1/500) was used as the secondary. DAPI was used for nuclei staining (blue).

This image is courtesy of an Abreview submitted by Dr Vladimir Milenkovic

AbReview16564****

Immunoprecipitation - Anti-6X His tag® antibody [4D11] (AB5000)

ab5000 Immunoprecipitate in human HEK293 whole cell lysate. 25μg of cell lysate incubated with primary antibody (undiluted) and matrix (Protein G) for 16 hours at 4°C. For western blotting an undiluted HRP-Goat anti-rabbit IgG polyclonal was used. Beta3-His fusion protein was immunoprecipitated using anti His Ab, and specific beta3a band (56kDa) was detected using anti beta3 Ab

Lane 1. Lysate of HEK293 cells expressing beta3-His (CACNB3)

Lane 2. IP with anti His Ab

Lane 3. Non bound fraction

All lanes:

Immunoprecipitation - Anti-6X His tag® antibody [4D11] (ab5000)

false

This image is courtesy of an Abreview submitted by Dr Vladimir Milenkovic

Project

Western blot - Anti-6X His tag® antibody [4D11] (AB5000)

All lanes:

Western blot - Anti-6X His tag® antibody [4D11] (ab5000) at 1/1000 dilution

All lanes:

6X His Tagged Protein at 1 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) (ab65485) at 1/3000 dilution

true

Exposure time: 30s

AbReview16563****

Western blot - Anti-6X His tag® antibody [4D11] (AB5000)

All lanes:

Western blot - Anti-6X His tag® antibody [4D11] (ab5000) at 1/2000 dilution

Lane 1:

Lysates prepared from HEK-293 cells

Lane 2:

Lysates prepared from HEK-293 cells transfected with Beta3-His (CACNB3)

Secondary

All lanes:

HRP-conjugated goat anit-mouse IgG polyclonal at 1/5000 dilution

false

This image is courtesy of an Abreview submitted by Dr Vladimir Milenkovic

不同偶联物与剂型 (1)

HRP

HRP Anti-6X His tag® antibody [4D11]

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

4D11

亚型

IgG2a

轻链类型

kappa

不含载体蛋白

应用

ELISA, AP, ICC/IF, Flow Cyt, IP, Dot, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

表位

This antibody was shown to recognize an epitope (HHHHHHGS) encoded by the polylinker of several expression vectors (including pDS:RBSII, pQE-30, and pQE-50).

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "AP" : {"fullname" : "Affinity Purification", "shortname":"AP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "See Abreview.", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/products/primary-antibodies/mouse-igg2c-kappa-monoclonal-18c8bc7ad10-isotype-control-ab170191'>ab170191</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "AP-species-checked": "guaranteed", "AP-species-dilution-info": "", "AP-species-notes": "" } } }

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产品详情

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

HIS-TAG® is a trademark of EMD Biosciences, Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification

存储溶液

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 6.97% L-Arginine

运输条件

Blue Ice

分装信息

Add glycerol to a final volume of 50% for extra stability and aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The 6X His tag is a sequence commonly used for protein purification detection and immobilization. It consists of six histidine residues that bind to metal ions like nickel or cobalt. This makes it a highly efficient tag for affinity chromatography. His tag antibodies such as anti-His HRP facilitate the detection of His-tagged proteins in various assays. Being a small tag with a typical molecular weight of about 0.84 kDa it minimally interferes with protein function. Many proteins expressed in E. coli and other expression systems carry the 6X His tag for ease of purification and analysis.

Biological function summary

Proteins tagged with the 6X His tag often play roles in studies involving recombinant proteins. The tag itself does not alter the biological function of the target protein. It is not part of any natural protein complex but aids in the study of such complexes. Researchers use His tag antibodies like anti-His to specifically target and study these tagged proteins without cross-reactivity to other proteins.

Pathways

The incorporation of the 6X His tag into proteins allows them to be studied more efficiently in various biological pathways. It can be part of pathways like signal transduction or metabolic pathways. When tagged proteins interact with other molecules such as kinases or receptors researchers can track these interactions using the tag and anti-Histidine antibodies. This technique informs on proteins that are hard to purify due to low abundance or instability.

The 6X His tag itself does not have direct implications. However proteins of interest in disease models often carry the tag which allows researchers to purify and study them in detail. For example cancer-related proteins may be expressed with a His tag to understand their role in tumorigenesis. Similarly proteins involved in metabolic disorders could be tagged which helps in analyzing specific interactions or functions disrupted in diseases.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

See full target information 6X His tag®

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文献 (32)

Recent publications for all applications. Explore the full list and refine your search

Biomolecules & biomedicine 24:1424-1434 PubMed38752985

2024

Potent antitumor activity of a bispecific T-cell engager antibody targeting the intracellular antigen KRAS G12V.

Applications

Unspecified application

Species

Unspecified reactive species

Changchang Lu,Lu Zou,Qiaoli Wang,Mengna Sun,Tianyu Shi,Shuang Xu,Fanyan Meng,Juan Du

Genes & diseases 10:2622-2638 PubMed37554218

2023

PRMT1 promotes the proliferation and metastasis of gastric cancer cells by recruiting MLXIP for the transcriptional activation of the β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Wang,Shitong Chen,Shihan Peng,Xujun Zhou,Houyi Tang,Hanghua Liang,Xi Zhong,He Yang,Xiaoxue Ke,MuHan Lü,Hongjuan Cui

Journal of lipid research 62:100115 PubMed34508728

2021

A simple, rapid, and sensitive fluorescence-based method to assess triacylglycerol hydrolase activity.

Applications

Unspecified application

Species

Unspecified reactive species

Sujith Rajan,Hazel C de Guzman,Thomas Palaia,Ira J Goldberg,M Mahmood Hussain

Frontiers in cell and developmental biology 9:646113 PubMed34307343

2021

GLP-1-Induced AMPK Activation Inhibits PARP-1 and Promotes LXR-Mediated ABCA1 Expression to Protect Pancreatic β-Cells Against Cholesterol-Induced Toxicity Through Cholesterol Efflux.

Applications

Unspecified application

Species

Unspecified reactive species

Rao Li,Xulong Sun,Pengzhou Li,Weizheng Li,Lei Zhao,Liyong Zhu,Shaihong Zhu

The Journal of clinical investigation 131: PubMed33661764

2021

SUMOylation promotes extracellular vesicle-mediated transmission of lncRNA ELNAT1 and lymph node metastasis in bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Changhao Chen,Hanhao Zheng,Yuming Luo,Yao Kong,Mingjie An,Yuting Li,Wang He,Bowen Gao,Yue Zhao,Hao Huang,Jian Huang,Tianxin Lin

Frontiers in cell and developmental biology 8:559486 PubMed33324631

2020

S100A10 Accelerates Aerobic Glycolysis and Malignant Growth by Activating mTOR-Signaling Pathway in Gastric Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Li,Xiao-Yu Li,Li-Xiang Li,Ru-Chen Zhou,Yinhe Sikong,Xiang Gu,Bi-Ying Jin,Bing Li,Yan-Qing Li,Xiu-Li Zuo

Autophagy 17:1714-1728 PubMed32543276

2020

PKD2/polycystin-2 induces autophagy by forming a complex with BECN1.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Peña-Oyarzun,Marcelo Rodriguez-Peña,Francesca Burgos-Bravo,Angelo Vergara,Catalina Kretschmar,Cristian Sotomayor-Flores,Cesar A Ramirez-Sarmiento,Humbert De Smedt,Montserrat Reyes,William Perez,Vicente A Torres,Eugenia Morselli,Francisco Altamirano,Christian A M Wilson,Joseph A Hill,Sergio Lavandero,Alfredo Criollo

Oncoimmunology 9:1747688 PubMed32363119

2020

CD19-targeting fusion protein combined with PD1 antibody enhances anti-tumor immunity in mouse models.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuangwei Lv,Ping Zhang,Dandan Li,Mengting Qin,Longzhu Nie,Xiaoqian Wang,Li Ai,Zhaozu Feng,Woodvine Otieno Odhiambo,Yunfeng Ma,Yanhong Ji

eLife 8: PubMed31868578

2019

Aberrant calcium channel splicing drives defects in cortical differentiation in Timothy syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Georgia Panagiotakos,Christos Haveles,Arpana Arjun,Ralitsa Petrova,Anshul Rana,Thomas Portmann,Sergiu P Paşca,Theo D Palmer,Ricardo E Dolmetsch

Drug testing and analysis 11:1675-1697 PubMed31758732

2019

Detection of black market follistatin 344.

Applications

Unspecified application

Species

Unspecified reactive species

Christian Reichel,Günter Gmeiner,Mario Thevis

View all publications

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Anti-6X His tag® 抗体 [4D11]