重组Anti-68kDa Neurofilament/NF-L抗体[Nfl21] - BSA and Azide free (ab273479)
![Western blot - Anti-68kDa Neurofilament/NF-L antibody [Nfl21] - BSA and Azide free (ab273479)](https://www.abcam.cn/ps/products/273/ab273479/Images/ab273479-411647-anti-neurofilament-light-antibody-nfl21-western-blot-sknbe.jpg "Western blot - Anti-68kDa Neurofilament/NF-L antibody [Nfl21] - BSA and Azide free (ab273479)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [Nfl21] to 68kDa Neurofilament/NF-L - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-68kDa Neurofilament/NF-L抗体[Nfl21] - BSA and Azide free
参阅全部 68kDa Neurofilament/NF-L 一抗 -
描述
小鼠单克隆抗体[Nfl21] to 68kDa Neurofilament/NF-L - BSA and Azide free -
宿主
Mouse -
经测试应用
适用于: IHC-P, WBmore details
不适用于: ICC/IF or IHC-Fr -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: SK-N-BE(2) whole cell lysate. Human cerebrospinal fluid. IHC-P: Human, mouse and rat cerebrum tissue.
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常规说明
ab273479 is the carrier-free version of ab273441.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
Constituent: 100% PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
Nfl21 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab273479于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
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| WB |
Use at an assay dependent concentration. Predicted molecular weight: 62 kDa.
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| 说明 |
|---|
|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 62 kDa. |
靶标
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功能
Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. -
疾病相关
Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 1F (CMT1F) [MIM:607734]. CMT1F is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT1 group are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. CMT1F is characterized by onset in infancy or childhood (range 1 to 13 years).
Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 2E (CMT2E) [MIM:607684]. CMT2E is an autosomal dominant form of Charcot-Marie-Tooth disease type 2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. -
序列相似性
Belongs to the intermediate filament family. -
结构域
The extra mass and high charge density that distinguish the neurofilament proteins from all other intermediate filament proteins are due to the tailpiece extensions. This region may form a charged scaffolding structure suitable for interaction with other neuronal components or ions. -
翻译后修饰
O-glycosylated.
Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization. - Information by UniProt
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数据库链接
- Entrez Gene: 4747 Human
- Entrez Gene: 18039 Mouse
- Entrez Gene: 83613 Rat
- Omim: 162280 Human
- SwissProt: P07196 Human
- SwissProt: P08551 Mouse
- SwissProt: P19527 Rat
- Unigene: 521461 Human
see all -
别名
- 68 kDa neurofilament protein antibody
- 68kDa Neurofilament antibody
- 68kDa neurofilament protein antibody
see all
图片
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Western blot - Anti-68kDa Neurofilament/NF-L antibody [Nfl21] - BSA and Azide free (ab273479)All lanes : Anti-68kDa Neurofilament/NF-L antibody [Nfl21] (ab273441) at 1/1000 dilution
Lane 1 : SK-N-BE(2) (human neuroblastoma neuroblast), whole cell lysate at 20 µg
Lane 2 : Human cerebrospinal fluid at 40 µl
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution
Predicted band size: 62 kDaThis data was developed using ab273441 the same antibody clone in a different buffer formulation.
This blot was developed using a higher sensitivity ECL substrate.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1: 37 seconds; Lane 2: 3 minutes.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L antibody - BSA and Azide free (ab273479)This data was developed using ab273441 the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Neurofilament light with ab273441 at 1/500 (2.116 µg/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on human cerebrum is observed. The section was incubated with ab273441 for 30 mins at room temperature and blocked mouse IgG with specific antibody ab125913 for 8min. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Western blot - Anti-68kDa Neurofilament/NF-L antibody [Nfl21] - BSA and Azide free (ab273479)](https://www.abcam.cn/ps/products/273/ab273479/Images/ab273479-411648-anti-neurofilament-light-antibody-nfl21-western-blot-mouse-hippocampus-tissue-lysate-20-ug.jpg)
Western blot - Anti-68kDa Neurofilament/NF-L antibody [Nfl21] - BSA and Azide free (ab273479)All lanes : Anti-68kDa Neurofilament/NF-L antibody [Nfl21] (ab273441) at 1/1000 dilution
Lane 1 : Mouse hippocampus tissue lysate
Lane 2 : Mouse liver tissue lysate
Lane 3 : Rat brain tissue lysate
Lane 4 : Rat hippocampus tissue lysate
Lane 5 : Rat liver tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-mouse IgG for IP (HRP) (ab131368) at 1/1000 dilution
Predicted band size: 62 kDaThis data was developed using ab273441 the same antibody clone in a different buffer formulation.
Negative control: liver (PMID: 2120242).
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1-2: 10 seconds; Lane 3-5: 37 seconds.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L antibody - BSA and Azide free (ab273479)This data was developed using ab273441 the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Neurofilament light with ab273441 at 1/500 (2.116 µg/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on mouse cerebrum is observed. The section was incubated with ab273441 for 30 mins at room temperature and blocked mouse IgG with specific antibody ab125913 for 8min. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L antibody - BSA and Azide free (ab273479)This data was developed using ab273441 the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Neurofilament light with ab273441 at 1/500 (2.116 µg/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on rat cerebrum is observed. The section was incubated with ab273441 for 30 mins at room temperature and blocked mouse IgG with specific antibody ab125913 for 8min. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Anti-68kDa Neurofilament/NF-L antibody [Nfl21] - BSA and Azide free (ab273479)](https://www.abcam.cn/ps/products/273/ab273479/Images/ab273479-6-benefits-of-recombinant-antibodies.png)
Anti-68kDa Neurofilament/NF-L antibody [Nfl21] - BSA and Azide free (ab273479)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab273479 尚未被引用在任何文献中。
