Anti-68kDa Neurofilament/NF-L抗体[DA2] (ab7255)
![Western blot - Anti-68kDa Neurofilament/NF-L antibody [DA2] (ab7255)](https://www.abcam.cn/ps/products/7/ab7255/Images/ab7255-313628-anti-68kda-neurofilament-nf-l-antibody-da2-western-blot.jpg "Western blot - Anti-68kDa Neurofilament/NF-L antibody [DA2] (ab7255)")
Key features and details
- Mouse monoclonal [DA2] to 68kDa Neurofilament/NF-L
- Suitable for: Flow Cyt, ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
概述
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产品名称
Anti-68kDa Neurofilament/NF-L抗体[DA2]
参阅全部 68kDa Neurofilament/NF-L 一抗 -
描述
小鼠单克隆抗体[DA2] to 68kDa Neurofilament/NF-L -
宿主
Mouse -
特异性
Specifically recognizes the light neurofilament subunit or 68kDa Neurofilament/NF-L.
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经测试应用
适用于: Flow Cyt, ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Bird, Mammals
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免疫原
Full length native protein (purified) corresponding to Pig 68kDa Neurofilament/NF-L. A preparation of enzymatically dephosphorylated pig neurofilaments including NF-L, NF-M and NF-H. Screening was by ELISA on the immunogen followed by immunofluorescence microscopy.
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表位
Epitope mapped to a short peptide in the C-terminal “tail” region of the molecule within the human sequence SYYTSHVQEEQIEVE, amino acids 441-455. -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.033% Sodium azide
Constituent: Tissue culture supernatant -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
DA2 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
1/100.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| ICC/IF | (1) |
1/1000.
For unpurifed use at : 1/100 - 1/500 |
| WB |
1/5000 - 1/10000.
|
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| IHC-P |
1/1000.
(works on mildly formalin fixed sections) |
| 说明 |
|---|
|
Flow Cyt
1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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ICC/IF
1/1000. For unpurifed use at : 1/100 - 1/500 |
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WB
1/5000 - 1/10000. |
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IHC-P
1/1000. (works on mildly formalin fixed sections) |
靶标
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功能
Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. -
疾病相关
Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 1F (CMT1F) [MIM:607734]. CMT1F is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT1 group are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. CMT1F is characterized by onset in infancy or childhood (range 1 to 13 years).
Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 2E (CMT2E) [MIM:607684]. CMT2E is an autosomal dominant form of Charcot-Marie-Tooth disease type 2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. -
序列相似性
Belongs to the intermediate filament family. -
结构域
The extra mass and high charge density that distinguish the neurofilament proteins from all other intermediate filament proteins are due to the tailpiece extensions. This region may form a charged scaffolding structure suitable for interaction with other neuronal components or ions. -
翻译后修饰
O-glycosylated.
Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization. - Information by UniProt
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数据库链接
- Entrez Gene: 4747 Human
- Entrez Gene: 18039 Mouse
- Entrez Gene: 83613 Rat
- Omim: 162280 Human
- SwissProt: P07196 Human
- SwissProt: P08551 Mouse
- SwissProt: P19527 Rat
- Unigene: 521461 Human
see all -
别名
- 68 kDa neurofilament protein antibody
- 68kDa Neurofilament antibody
- 68kDa neurofilament protein antibody
see all
图片
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Western blot - Anti-68kDa Neurofilament/NF-L antibody [DA2] (ab7255)All lanes : Anti-68kDa Neurofilament/NF-L antibody [DA2] (ab7255) at 1/5000 dilution
Lane 1 : rat brain whole tissue lysates
Lane 2 : rat spinal cord whole tissue lysates
Lane 3 : mouse brain whole tissue lysates
Lane 4 : mouse spinal cord whole tissue lysates -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L antibody [DA2] (ab7255)](https://www.abcam.cn/ps/products/7/ab7255/Images/ab7255-12676-anti-68kda-neurofilament-nf-l-antibody-da2-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L antibody [DA2] (ab7255)IHC image of ab7255 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab7255, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
![Immunocytochemistry/ Immunofluorescence - Anti-68kDa Neurofilament/NF-L antibody [DA2] (ab7255)](https://www.abcam.cn/ps/products/7/ab7255/Images/ab7255-313636-anti-68kda-neurofilament-nf-l-antibody-da2-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-68kDa Neurofilament/NF-L antibody [DA2] (ab7255)Ab7255 staining 68kDa Neurofilament/NF-L in rat frontal cortex sections by Immunocytochemistry/Immunofluorescence. Samples were incubated with primary antibody at 1/5000 dilution (red) and chicken polyclonal to GFAP.
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![Western blot - Anti-68kDa Neurofilament/NF-L antibody [DA2] (ab7255)](https://www.abcam.cn/ps/products/7/ab7255/Images/ab7255-12677-anti-68kda-neurofilament-nf-l-antibody-da2-western-blot.jpg)
Western blot - Anti-68kDa Neurofilament/NF-L antibody [DA2] (ab7255)Lane 1 : Coomassie Blue stain
Lane 2 : Heavy neurofilament antibody
Lane 3 : Anti-160 kD Neurofilament Medium antibody [3H11] (ab7256)
Lane 4 : Anti-68kDa Neurofilament/NF-L antibody [DA2] (ab7255)
Lane 5 : alpha Internexin antibody
Lane 6 : GFAP antibody
All lanes : Cytoskeletal homogenates of rat spinal cord -
![Flow Cytometry - Anti-68kDa Neurofilament/NF-L antibody [DA2] (ab7255)](https://www.abcam.cn/ps/products/7/ab7255/Images/ab7255-12675-anti-68kda-neurofilament-nf-l-antibody-da2-flow-cytometry.jpg)
Flow Cytometry - Anti-68kDa Neurofilament/NF-L antibody [DA2] (ab7255)Overlay histogram showing SH-SY5Y cells stained with ab7255 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7255, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab7255 被引用在 10 文献中.
- Sano T et al. Comparative analysis of neurofilament light chain levels in the serum and cerebrospinal fluid in rats subjected to partial sciatic nerve ligation. J Toxicol Pathol 36:145-149 (2023). PubMed: 37101960
- Elseweidy MM et al. Pentoxifylline as Add-On Treatment to Donepezil in Copper Sulphate-Induced Alzheimer's Disease-Like Neurodegeneration in Rats. Neurotox Res 41:546-558 (2023). PubMed: 37821782
- Zilocchi M et al. Co-fractionation-mass spectrometry to characterize native mitochondrial protein assemblies in mammalian neurons and brain. Nat Protoc 18:3918-3973 (2023). PubMed: 37985878
- Nass SR et al. Neurodegeneration Within the Amygdala Is Differentially Induced by Opioid and HIV-1 Tat Exposure. Front Neurosci 16:804774 (2022). PubMed: 35600626
- Fayazi M et al. In-vitro construction of endometrial-like epithelium using CD146+ mesenchymal cells derived from human endometrium. Reprod Biomed Online 35:241-252 (2017). PubMed: 28668272
- Rupprecht A et al. Uncoupling protein 2 and 4 expression pattern during stem cell differentiation provides new insight into their putative function. PLoS One 9:e88474 (2014). WB ; Mouse . PubMed: 24523901
- Roher AE et al. Subjects harboring presenilin familial Alzheimer's disease mutations exhibit diverse white matter biochemistry alterations. Am J Neurodegener Dis 2:187-207 (2013). PubMed: 24093083
- Dorval V et al. Gene network and pathway analysis of mice with conditional ablation of Dicer in post-mitotic neurons. PLoS One 7:e44060 (2012). PubMed: 22952873
- Zhang L et al. {gamma}-Diketone Axonopathy: Analyses of Cytoskeletal Motors and Highways in CNS Myelinated Axons. Toxicol Sci 117:180-9 (2010). WB ; Rat . PubMed: 20554699
- Edson KJ et al. Identification of a tektin-like protein associated with neurofilaments in the developing chick nervous system. J Neurosci Res 30:105-15 (1991). PubMed: 1795395