重组Anti-68kDa Neurofilament/NF-L抗体[EPR22035-112] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] - BSA and Azide free (AB238420)

Immunohistochemical analysis of paraffin-embedded human cerebellum tissue labeling 68kDa Neurofilament/NF-L with ab223343 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining of nerve fibers in human cerebellum (PMID : 28190929) is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223343).

• I-ELISA

Lab

Indirect ELISA - Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] - BSA and Azide free (AB238420)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223343). ELISA using ab223343 at 1000-0 ng/mL antibody concentrations and antigen human Neurofilament light polypeptide at 1000 ng/mL. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

• IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] - BSA and Azide free (AB238420)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen sectioned rat cerebellum tissue labeling 68kDa Neurofilament/NF-L with ab223343 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution (green). Positive staining in rat cerebellum is observed. The nuclear counter stain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223343).

• IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] - BSA and Azide free (AB238420)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen sectioned mouse liver tissue labeling 68kDa Neurofilament/NF-L with ab223343 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution. No staining observed in mouse liver. The nuclear counter stain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20).

Negative control : mouse liver (PMID : 2120242).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223343).

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] - BSA and Azide free (AB238420)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen sectioned mouse cerebellum tissue labeling 68kDa Neurofilament/NF-L with ab223343 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution (green). Positive staining in mouse cerebellum (PMID : 17598884) is observed. The nuclear counter stain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223343).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] - BSA and Azide free (AB238420)

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue labeling 68kDa Neurofilament/NF-L with ab223343 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining of nerve fibers in mouse cerebellum (PMID : 28190929) is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223343).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] - BSA and Azide free (AB238420)

Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labeling 68kDa Neurofilament / NF-L with ab223343 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining of nerve fibers in rat cerebellum (PMID : 28190929) is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223343).

• IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] - BSA and Azide free (AB238420)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen sectioned rat liver tissue labeling 68kDa Neurofilament/NF-L with ab223343 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution. No staining in rat liver is observed. The nuclear counter stain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20).

Negative control : Rat liver (PMID : 2120242).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223343).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] - BSA and Azide free (AB238420)

Immunocytochemistry/ Immunofluorescence analysis of mouse primary neuron cells labeling 68kDa Neurofilament/NF-L with purified ab223343 at 1/50 (9.4μg/mL). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab11267 Anti-MAP2 antibody [HM-2]; ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223343).

• IP

Unknown

Immunoprecipitation - Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] - BSA and Azide free (AB238420)

68kDa Neurofilament/NF-L was immunoprecipitated from 0.35 mg of mouse brain lysate with ab223343 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab223343 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1 : Mouse brain lysate 10 μg (Input).
Lane 2 : ab223343 IP in mouse brain lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab223343 in mouse brain lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 10 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223343).

All lanes:

Immunoprecipitation - Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] (<a href='/products/primary-antibodies/68kda-neurofilament-nf-l-antibody-epr22035-112-ab223343'>ab223343</a>)

Predicted band size: 62 kDa

Observed band size: 68 kDa

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