AB236122

重组Anti-68kDa Neurofilament/NF-L抗体[EP675Y] - BSA and Azide free

Anti-68kDa Neurofilament/NF-L antibody [EP675Y] - BSA and Azide free

RabMAb
Recombinant
了解详情

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Recombinant Monoclonal 68kDa Neurofilament/NF-L antibody. Carrier free. Suitable for IP, WB and reacts with Human, Rat, Mouse samples. Cited in 1 publication.

查看别名

NF68, NFL, NEFL, Neurofilament light polypeptide, NF-L, 68 kDa neurofilament protein, Neurofilament triplet L protein

2 Images

Lab

Immunoprecipitation - Anti-68kDa Neurofilament/NF-L antibody [EP675Y] - BSA and Azide free (AB236122)

Purified ab52989 at 1 : 20 dilution (2μg) immunoprecipitating 68kDa Neurofilament/NF-L in Human brain lysate.
Lane 1 (input) : Human brain lysate 10μg
Lane 2 (+) : ab52989 + Human brain lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab52989 in Human brain lysate.
VeriBlot for IP Detection Reagent (HRP)(ab131366) (1 : 1000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 68 kDa

All lanes:

Immunoprecipitation - Anti-68kDa Neurofilament/NF-L antibody [EP675Y] (<a href='/products/primary-antibodies/68kda-neurofilament-nf-l-antibody-ep675y-ab52989'>ab52989</a>)

Predicted band size: 62 kDa

false

Unknown

Western blot - Anti-68kDa Neurofilament/NF-L antibody [EP675Y] - BSA and Azide free (AB236122)

This data was developed using ab52989, the same antibody clone in a different buffer formulation.

Blocking buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-68kDa Neurofilament/NF-L antibody [EP675Y] (<a href='/products/primary-antibodies/68kda-neurofilament-nf-l-antibody-ep675y-ab52989'>ab52989</a>) at 1/1000 dilution

Lane 1:

Human brain lysate at 15 µg

Lane 2:

Mouse brain lysate at 15 µg

Lane 3:

Rat brain lysate at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 62 kDa

Observed band size: 68 kDa

false

不同偶联物与剂型 (1)

Unconjugated

Anti-68kDa Neurofilament/NF-L antibody [EP675Y]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EP675Y

亚型

IgG

不含载体蛋白

Yes

反应种属

Mouse, Rat, Human

应用

WB, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

产品详情

ab236122 is the carrier-free version of ab52989.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The 68kDa Neurofilament also known as NF-L or neurofilament light plays a mechanical role in maintaining the structural integrity of neurons. This protein is characterized by its mass of 68 kilodaltons. It expresses specifically in the cytoplasm of neurons where it forms a critical part of the neurofilament protein triplet along with NF-M and NF-H. These components collectively form the intermediate filaments found in the neuron axons providing essential structural support.

Biological function summary

The neurofilament protein functions to stabilize the axonal diameter thereby affecting neuronal conductivity. It integrates into a complex with other neurofilament proteins and related molecules forming cross-bridges that aid in strengthening the cellular structure. This protein-protein interaction is vital for normal neuronal function and efficient nerve signal transmission.

Pathways

Neurofilament proteins contribute to cellular processes within the nervous system. It participates in the axonal transport pathway which includes the transport of synaptic vesicles and organelles along axons. This movement is facilitated by interactions with motor proteins like kinesin and dynein which help in moving these materials to their destinations. Additionally NF-L connects to actin filaments collaborating to sustain neuronal shape and function.

Neurofilament proteins have links to conditions such as amyotrophic lateral sclerosis (ALS) and Alzheimer's disease. Elevated levels of NF-L are often observed in ALS suggesting neuronal damage as axonal injury raises the protein's presence in the cerebrospinal fluid. Similarly in Alzheimer's disease aberrant interaction of tau protein with neurofilament proteins disrupts neuronal function. These connections highlight NF-L's potential as a biomarker for neural injury and neurodegenerative diseases.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Neurofilaments usually contain three intermediate filament proteins : NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).

See full target information NEFL

style

left-column

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of neuroinflammation 17:252 PubMed32859212

2020

Transgenic inhibition of astroglial NF-κB restrains the neuroinflammatory and neurodegenerative outcomes of experimental mouse glaucoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangjun Yang,Qun Zeng,Mine Barış,Gülgün Tezel

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-68kDa Neurofilament/NF-L抗体[EP675Y] - BSA and Azide free