AB52989

重组Anti-68kDa Neurofilament/NF-L抗体[EP675Y]

Anti-68kDa Neurofilament/NF-L antibody [EP675Y]

20ul selling size
RabMAb
Recombinant
了解详情

Be the first to review this product! Submit a review

(6 Publications)

Rabbit Recombinant Monoclonal 68kDa Neurofilament/NF-L antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 6 publications.

查看别名

NF68, NFL, NEFL, Neurofilament light polypeptide, NF-L, 68 kDa neurofilament protein, Neurofilament triplet L protein

2 Images

Lab

Immunoprecipitation - Anti-68kDa Neurofilament/NF-L antibody [EP675Y] (AB52989)

Purified ab52989 at 1 : 20 dilution (2μg) immunoprecipitating 68kDa Neurofilament/NF-L in Human brain lysate.
Lane 1 (input) : Human brain lysate 10μg
Lane 2 (+) : ab52989 + Human brain lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab52989 in Human brain lysate.
VeriBlot for IP Detection Reagent (HRP)(ab131366) (1 : 1000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 68 kDa

All lanes:

Immunoprecipitation - Anti-68kDa Neurofilament/NF-L antibody [EP675Y] (ab52989)

Predicted band size: 62 kDa

false

Unknown

Western blot - Anti-68kDa Neurofilament/NF-L antibody [EP675Y] (AB52989)

Blocking buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-68kDa Neurofilament/NF-L antibody [EP675Y] (ab52989) at 1/1000 dilution

Lane 1:

Human brain lysate at 15 µg

Lane 2:

Mouse brain lysate at 15 µg

Lane 3:

Rat brain lysate at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 62 kDa

Observed band size: 68 kDa

false

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EP675Y

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

IP, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/20", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

style

left-column

产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

储存信息

Stable for 12 months at -20°C

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The 68kDa Neurofilament also known as NF-L or neurofilament light plays a mechanical role in maintaining the structural integrity of neurons. This protein is characterized by its mass of 68 kilodaltons. It expresses specifically in the cytoplasm of neurons where it forms a critical part of the neurofilament protein triplet along with NF-M and NF-H. These components collectively form the intermediate filaments found in the neuron axons providing essential structural support.

Biological function summary

The neurofilament protein functions to stabilize the axonal diameter thereby affecting neuronal conductivity. It integrates into a complex with other neurofilament proteins and related molecules forming cross-bridges that aid in strengthening the cellular structure. This protein-protein interaction is vital for normal neuronal function and efficient nerve signal transmission.

Pathways

Neurofilament proteins contribute to cellular processes within the nervous system. It participates in the axonal transport pathway which includes the transport of synaptic vesicles and organelles along axons. This movement is facilitated by interactions with motor proteins like kinesin and dynein which help in moving these materials to their destinations. Additionally NF-L connects to actin filaments collaborating to sustain neuronal shape and function.

Neurofilament proteins have links to conditions such as amyotrophic lateral sclerosis (ALS) and Alzheimer's disease. Elevated levels of NF-L are often observed in ALS suggesting neuronal damage as axonal injury raises the protein's presence in the cerebrospinal fluid. Similarly in Alzheimer's disease aberrant interaction of tau protein with neurofilament proteins disrupts neuronal function. These connections highlight NF-L's potential as a biomarker for neural injury and neurodegenerative diseases.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Neurofilaments usually contain three intermediate filament proteins : NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).

See full target information NEFL

style

left-column

文献 (6)

Recent publications for all applications. Explore the full list and refine your search

Brain : a journal of neurology 147:4227-4239 PubMed39008620

2024

Customized antisense oligonucleotide-based therapy for neurofilament-associated Charcot-Marie-Tooth disease.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica Medina,Adriana Rebelo,Matt C Danzi,Elizabeth H Jacobs,Isaac R L Xu,Kathleen P Ahrens,Sitong Chen,Jacquelyn Raposo,Christopher Yanick,Stephan Zuchner,Mario A Saporta

Biological research 56:34 PubMed37349842

2023

Functional defects in hiPSCs-derived cardiomyocytes from patients with a PLEKHM2-mutation associated with dilated cardiomyopathy and left ventricular non-compaction.

Applications

Unspecified application

Species

Unspecified reactive species

Nataly Korover,Sharon Etzion,Alexander Cherniak,Tatiana Rabinski,Aviva Levitas,Yoram Etzion,Rivka Ofir,Ruti Parvari,Smadar Cohen

Brain and behavior 12:e2788 PubMed36282532

2022

MiR-30b-5p attenuates the inflammatory response and facilitates the functional recovery of spinal cord injury by targeting the NEFL/mTOR pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Zhang,Duojun Wang,Jinyu Tong,Jianguo Fang,Zaijun Lin

Antioxidants (Basel, Switzerland) 10: PubMed33803289

2021

Dimethyl Fumarate, an Approved Multiple Sclerosis Treatment, Reduces Brain Oxidative Stress in SIV-Infected Rhesus Macaques: Potential Therapeutic Repurposing for HIV Neuroprotection.

Applications

Unspecified application

Species

Unspecified reactive species

Yoelvis Garcia-Mesa,He N Xu,Patricia Vance,Analise L Gruenewald,Rolando Garza,Cecily Midkiff,Xavier Alvarez-Hernandez,David J Irwin,Alexander J Gill,Dennis L Kolson

Journal of virology 94: PubMed32669339

2020

Regional Brain Recovery from Acute Synaptic Injury in Simian Immunodeficiency Virus-Infected Rhesus Macaques Associates with Heme Oxygenase Isoform Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Yoelvis Garcia-Mesa,Rolando Garza,Maria E Diaz Ortiz,Analise L Gruenewald,Brandon L Bastien,Rebecca Lobrovich,David J Irwin,Michael R Betts,Guido Silvestri,Dennis L Kolson

The Journal of neuroscience : the official journal of the Society for Neuroscience 29:6186-95 PubMed19439596

2009

Neuronal PPARgamma deficiency increases susceptibility to brain damage after cerebral ischemia.

Applications

Species

Mouse

Xiurong Zhao,Roger Strong,Jie Zhang,Guanghua Sun,Joe Z Tsien,Zhenzhong Cui,James C Grotta,Jaroslaw Aronowski

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-68kDa Neurofilament/NF-L抗体[EP675Y]

Anti-68kDa Neurofilament/NF-L antibody [EP675Y]

Immunoprecipitation - Anti-68kDa Neurofilament/NF-L antibody [EP675Y] (AB52989)

Western blot - Anti-68kDa Neurofilament/NF-L antibody [EP675Y] (AB52989)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原