AB134460

Anti-68kDa Neurofilament/NF-L抗体

Name: 68kDa Neurofilament/NF-L抗体 (ab134460)| Abcam中文官网
Brand: Abcam Limited
SKU: AB134460
Price: 4797 CNY
Availability: InStock
Rating: 3 (1 reviews)

Anti-68kDa Neurofilament/NF-L antibody

(1 Review)

(2 Publications)

Chicken Polyclonal 68kDa Neurofilament/NF-L antibody. Suitable for IHC-FoFr, ICC/IF and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human NEFL conjugated to Keyhole Limpet Haemocyanin.

查看别名

NF68, NFL, NEFL, Neurofilament light polypeptide, NF-L, 68 kDa neurofilament protein, Neurofilament triplet L protein

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-68kDa Neurofilament/NF-L antibody (AB134460)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-68kDa Neurofilament/NF-L antibody (AB134460)

Immunocytochemistry/Immunofluorescence analysis of Rat Neurons stained with chicken anti-NF-H (green). The NF-H antibody was used at a dilution of 1/100000 using our standard fixation and staining procedure (in protocol section).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-68kDa Neurofilament/NF-L antibody (AB134460)

Immunocytochemistry/Immunofluorescence analysis of dissociated adult mouse cerebral cortical cells labelling 68kDa Neurofilament/NF-L (green) with ab134460. Cells were fixed with 4% paraformaldehyde. NFL immunoreactivity is red and yellow, Blue - DAPI nuclear stain.

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-68kDa Neurofilament/NF-L antibody (AB134460)

IHC-FoFr

Unknown

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-68kDa Neurofilament/NF-L antibody (AB134460)

Immunohistochemical analysis of adult mouse brain labelling 68kDa Neurofilament/NF-L in axons in the white matter of the cerebellum with ab134460 at 1/1000 dilution (brown staining).

关键信息

宿主种属

Chicken

克隆

Polyclonal

亚型

IgY

不含载体蛋白

反应种属

Mouse

应用

IHC-FoFr, ICC/IF

applications

免疫原

Synthetic Peptide within Human NEFL conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P07196

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFoFr" : {"fullname" : "Immunohistochemistry (PFA perfusion fixed frozen sections)", "shortname":"IHC-FoFr"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCFoFr-species-checked": "testedAndGuaranteed", "IHCFoFr-species-dilution-info": "1/1000 - 1/2000", "IHCFoFr-species-notes": "<p>Use 2% paraformaldehyde-fixed tissues.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000 - 1/2000", "ICCIF-species-notes": "<p>Use 2% paraformaldehyde-fixed cells.</p>" } } }

style

left-column

产品详情

Do not freeze the antibody unless you want to store it for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half its binding activity is lost.

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.3 mg/ml. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

存储溶液

pH: 7 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

运输条件

Blue Ice

储存信息

Avoid freeze / thaw cycle

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The 68kDa Neurofilament also known as NF-L or neurofilament light plays a mechanical role in maintaining the structural integrity of neurons. This protein is characterized by its mass of 68 kilodaltons. It expresses specifically in the cytoplasm of neurons where it forms a critical part of the neurofilament protein triplet along with NF-M and NF-H. These components collectively form the intermediate filaments found in the neuron axons providing essential structural support.

Biological function summary

The neurofilament protein functions to stabilize the axonal diameter thereby affecting neuronal conductivity. It integrates into a complex with other neurofilament proteins and related molecules forming cross-bridges that aid in strengthening the cellular structure. This protein-protein interaction is vital for normal neuronal function and efficient nerve signal transmission.

Pathways

Neurofilament proteins contribute to cellular processes within the nervous system. It participates in the axonal transport pathway which includes the transport of synaptic vesicles and organelles along axons. This movement is facilitated by interactions with motor proteins like kinesin and dynein which help in moving these materials to their destinations. Additionally NF-L connects to actin filaments collaborating to sustain neuronal shape and function.

Neurofilament proteins have links to conditions such as amyotrophic lateral sclerosis (ALS) and Alzheimer's disease. Elevated levels of NF-L are often observed in ALS suggesting neuronal damage as axonal injury raises the protein's presence in the cerebrospinal fluid. Similarly in Alzheimer's disease aberrant interaction of tau protein with neurofilament proteins disrupts neuronal function. These connections highlight NF-L's potential as a biomarker for neural injury and neurodegenerative diseases.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Neurofilaments usually contain three intermediate filament proteins : NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).

See full target information NEFL

style

left-column

文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Aging biology 1: PubMed38500536

2024

Early-Onset Hearing Loss in Mouse Models of Alzheimer's Disease and Increased DNA Damage in the Cochlea.

Applications

Unspecified application

Species

Unspecified reactive species

Jae-Hyeon Park,Burcin Duan Sahbaz,Komal Pekhale,Xixia Chu,Mustafa N Okur,Mhamed Grati,Kevin Isgrig,Wade Chien,Elena Chrysostomou,Lauren Sullivan,Deborah L Croteau,Uri Manor,Vilhelm A Bohr

Nature 605:152-159 PubMed35477759

2022

Neuroimmune cardiovascular interfaces control atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Sarajo K Mohanta,Li Peng,Yuanfang Li,Shu Lu,Ting Sun,Lorenzo Carnevale,Marialuisa Perrotta,Zhe Ma,Benjamin Förstera,Karen Stanic,Chuankai Zhang,Xi Zhang,Piotr Szczepaniak,Mariaelvy Bianchini,Borhan R Saeed,Raimondo Carnevale,Desheng Hu,Ryszard Nosalski,Fabio Pallante,Michael Beer,Donato Santovito,Ali Ertürk,Thomas C Mettenleiter,Barbara G Klupp,Remco T A Megens,Sabine Steffens,Jaroslav Pelisek,Hans-Henning Eckstein,Robert Kleemann,Livia Habenicht,Ziad Mallat,Jean-Baptiste Michel,Jürgen Bernhagen,Martin Dichgans,Giuseppe D'Agostino,Tomasz J Guzik,Peder S Olofsson,Changjun Yin,Christian Weber,Giuseppe Lembo,Daniela Carnevale,Andreas J R Habenicht

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-68kDa Neurofilament/NF-L抗体

Anti-68kDa Neurofilament/NF-L antibody

Immunocytochemistry/ Immunofluorescence - Anti-68kDa Neurofilament/NF-L antibody (AB134460)

Immunocytochemistry/ Immunofluorescence - Anti-68kDa Neurofilament/NF-L antibody (AB134460)

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-68kDa Neurofilament/NF-L antibody (AB134460)