重组Anti-5HT4 Receptor抗体[EPR28675-19] (ab317500)
![Western blot - Anti-5HT4 Receptor antibody [EPR28675-19] (ab317500)](https://www.abcam.cn/ps/products/317/ab317500/Images/ab317500-539011-anti-5ht4-receptor-antibody-epr28675-19-western-blot-human-testis-tissue-lysate20-ug.jpg "Western blot - Anti-5HT4 Receptor antibody [EPR28675-19] (ab317500)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR28675-19] to 5HT4 Receptor
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-5HT4 Receptor抗体[EPR28675-19]
参阅全部 5HT4 Receptor 一抗 -
描述
兔单克隆抗体[EPR28675-19] to 5HT4 Receptor -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt (Intra),ICC/IF,IHC-Fr,IHC-P or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human testis, Human placenta, Human striatum, Human tonsil, IMR-90, Human brain, Mouse placenta, Mouse striatum, Rat placenta, Rat striatum, HeLa, 293T, NIH/3T3 and PC-12 lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR28675-19 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab317500于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000.
|
| 说明 |
|---|
|
WB
1/1000. |
靶标
-
相关性
The 5HT4 Receptor is one of the several different receptors for 5- hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that stimulates adenylate cyclase. 5HT4a receptors may play a role in opioid-induced respiratory depression. -
细胞定位
Cell membrane; Multi-pass membrane protein. Endosome. Note: Interaction with SNX27 mediates recruitment to early endosomes, while interaction with SLC9A3R1 and EZR might target the protein to specialized subcellular regions, such as microvilli. -
数据库链接
- Entrez Gene: 3360 Human
- Entrez Gene: 15562 Mouse
- Entrez Gene: 25324 Rat
- Omim: 602164 Human
- SwissProt: Q13639 Human
- SwissProt: P97288 Mouse
- SwissProt: Q62758 Rat
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别名
- 5 HT 4 antibody
- 5 HT4 antibody
- 5 HT4R antibody
see all
图片
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Western blot - Anti-5HT4 Receptor antibody [EPR28675-19] (ab317500)All lanes : Anti-5HT4 Receptor antibody [EPR28675-19] (ab317500) at 1/1000 dilution
Lane 1 : Human testis tissue lysate
Lane 2 : Human placenta tissue lysate
Lane 3 : Human striatum tissue lysate
Lane 4 : Human skeletal muscle tissue lysate
Lane 5 : Human tonsil tissue lysate
Lane 6 : IMR-90 (human lung fibroblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 43 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression tissue: skeletal muscle (PMID: 15118808).
The identity of the bands higher than 50 kDa and lower than 37 kDa are unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
Exposure time: Lanes 1-4: 59 seconds; Lanes 5-6: 180 seconds.
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![Western blot - Anti-5HT4 Receptor antibody [EPR28675-19] (ab317500)](https://www.abcam.cn/ps/products/317/ab317500/Images/ab317500-539012-anti-5ht4-receptor-antibody-epr28675-19-western-blot-human-brain-tissue-lysate20-ug.jpg)
Western blot - Anti-5HT4 Receptor antibody [EPR28675-19] (ab317500)All lanes : Anti-5HT4 Receptor antibody [EPR28675-19] (ab317500) at 1/1000 dilution
Lane 1 : Human brain tissue lysate
Lane 2 : Human liver tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 43 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression tissue: liver (PMID: 15118808).
The identity of the band higher than 50 kDa is unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
-
![Western blot - Anti-5HT4 Receptor antibody [EPR28675-19] (ab317500)](https://www.abcam.cn/ps/products/317/ab317500/Images/ab317500-539013-anti-5ht4-receptor-antibody-epr28675-19-western-blot-mouse-placenta-tissue-lysate20-ug.jpg)
Western blot - Anti-5HT4 Receptor antibody [EPR28675-19] (ab317500)All lanes : Anti-5HT4 Receptor antibody [EPR28675-19] (ab317500) at 1/1000 dilution
Lane 1 : Mouse placenta tissue lysate
Lane 2 : Mouse striatum tissue lysate
Lane 3 : Mouse skeletal muscle tissue lysate
Lane 4 : Rat placenta tissue lysate
Lane 5 : Rat striatum tissue lysate
Lane 6 : Rat skeletal muscle tissue lysate
Lane 7 : Rat liver tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Observed band size: 43 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression tissue: Skeletal muscle, liver (PMID: 15118808).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
-
![Western blot - Anti-5HT4 Receptor antibody [EPR28675-19] (ab317500)](https://www.abcam.cn/ps/products/317/ab317500/Images/ab317500-539014-anti-5ht4-receptor-antibody-epr28675-19-western-blot-hela-human-cervical-adenocarcinoma-epithelial-cell-whole-cell-lysate20-ug.jpg)
Western blot - Anti-5HT4 Receptor antibody [EPR28675-19] (ab317500)All lanes : Anti-5HT4 Receptor antibody [EPR28675-19] (ab317500) at 1/1000 dilution
Lane 1 : HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : 293T (human embryonic kidney epithelial cell) whole cell lysate
Lane 3 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 4 : PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Observed band size: 43 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
The identity of the lower MW band at approximately 28 kDa (in lane 3) is unknown.
-
![Anti-5HT4 Receptor antibody [EPR28675-19] (ab317500)](https://www.abcam.cn/ps/products/317/ab317500/Images/ab317500-1-Benefits-of-Recombinant-antibodies.jpg)
Anti-5HT4 Receptor antibody [EPR28675-19] (ab317500)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
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